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Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase

Preliminary study on enzymatic hydrolysis process using combination of cellulase and ß 1-4 glucosidase on treated oil palm empty fruit bunch fibre (EFB) was performed. Crucial trends for parameters such as pH, temperature and substrate loading influencing the enzymatic hydrolysis of the treated EFB...

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Main Authors: Hamzah, Fazlena, Idris, Ani, Tan, Khai Shuan
Format: Article
Published: Elsevier Ltd. 2011
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/29370/
http://dx.doi.org/10.1016/j.biombioe.2010.11.020
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spelling my.utm.293702019-03-25T08:07:07Z http://eprints.utm.my/id/eprint/29370/ Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase Hamzah, Fazlena Idris, Ani Tan, Khai Shuan TP Chemical technology Preliminary study on enzymatic hydrolysis process using combination of cellulase and ß 1-4 glucosidase on treated oil palm empty fruit bunch fibre (EFB) was performed. Crucial trends for parameters such as pH, temperature and substrate loading influencing the enzymatic hydrolysis of the treated EFB fibre were also studied. Results revealed that a combination of both cellulase and ß 1-4 glucosidase with the ratio of 5:1 hydrolyzed more cellulose from treated EFB fibre and gave highest soluble glucose concentration up to 4 g L-1. The results indicated that as pH and temperature were increased the glucose produced also increased until pH 4.8 and 50 °C; beyond these values the reverse occurred. Glucose produced in the reaction increased with the increment in the substrate loading and maximum glucose concentration (2.7 g L-1) was achieved when 8% (wv-1) treated EFB was used as a substrate. Elsevier Ltd. 2011-03 Article PeerReviewed Hamzah, Fazlena and Idris, Ani and Tan, Khai Shuan (2011) Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase. Biomass and Bioenergy, 35 (3). pp. 1055-1059. ISSN 0961-9534 http://dx.doi.org/10.1016/j.biombioe.2010.11.020 DOI:10.1016/j.biombioe.2010.11.020
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Hamzah, Fazlena
Idris, Ani
Tan, Khai Shuan
Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
description Preliminary study on enzymatic hydrolysis process using combination of cellulase and ß 1-4 glucosidase on treated oil palm empty fruit bunch fibre (EFB) was performed. Crucial trends for parameters such as pH, temperature and substrate loading influencing the enzymatic hydrolysis of the treated EFB fibre were also studied. Results revealed that a combination of both cellulase and ß 1-4 glucosidase with the ratio of 5:1 hydrolyzed more cellulose from treated EFB fibre and gave highest soluble glucose concentration up to 4 g L-1. The results indicated that as pH and temperature were increased the glucose produced also increased until pH 4.8 and 50 °C; beyond these values the reverse occurred. Glucose produced in the reaction increased with the increment in the substrate loading and maximum glucose concentration (2.7 g L-1) was achieved when 8% (wv-1) treated EFB was used as a substrate.
format Article
author Hamzah, Fazlena
Idris, Ani
Tan, Khai Shuan
author_facet Hamzah, Fazlena
Idris, Ani
Tan, Khai Shuan
author_sort Hamzah, Fazlena
title Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
title_short Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
title_full Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
title_fullStr Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
title_full_unstemmed Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and ß1-4 glucosidase
title_sort preliminary study on enzymatic hydrolysis of treated oil palm (elaeis) empty fruit bunches fibre (efb) by using combination of cellulase and ß1-4 glucosidase
publisher Elsevier Ltd.
publishDate 2011
url http://eprints.utm.my/id/eprint/29370/
http://dx.doi.org/10.1016/j.biombioe.2010.11.020
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score 13.211869

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