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Metabolites profiling of heat treated whole palm oil extract

The chemically complex and diverse nature of the plant metabolome require several platform technologies to profile the entire range of metabolites. An ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) technique was used to profile and identify...

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Main Authors: Mohd. Fauzi, N. A., Sarmidi, Mohamad Roji, Chua, Lee Suan
Format: Article
Language:English
Published: Asian Network for Scientific Information 2011
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/29296/1/MohamadRojiSarmidi2011_MetabolitesProfilingofHeatTreatedWhole.pdf
http://eprints.utm.my/id/eprint/29296/
http://dx.doi.org/10.3923/jas.2011.2376.2381
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spelling my.utm.292962020-10-22T04:04:20Z http://eprints.utm.my/id/eprint/29296/ Metabolites profiling of heat treated whole palm oil extract Mohd. Fauzi, N. A. Sarmidi, Mohamad Roji Chua, Lee Suan QD Chemistry The chemically complex and diverse nature of the plant metabolome require several platform technologies to profile the entire range of metabolites. An ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) technique was used to profile and identify a set of small-molecule metabolites found in heat treated whole palm oil extract. An investigation was carried out on the effect of heat treatment on the yield, quality and metabolites profile for whole palm oil extract. Palm fruits were collected, cleaned and sterilized for 0, 20, 40 and 60 min. The pulps were then stripped from the sterilized fruits and later was pressed using laboratory scale expeller. The resulting puree was centrifuged at 4000 rpm for 20 min. The result shows that there was a significantly difference between sterilization time of 0 and 40 min in yield and quality. Of all, the highest oil yield of 19.9±0.21% (w/w) was obtained at 40 min of sterilization with DOBI value of 5.95±0.08 and FFA of 1.44±0.22%. The MarkerView software version 1.2.0.1 analysis of the UPLC-E SI -MS/MS preliminary experimental data demonstrated the distribution and identity of several compounds in the whole palm oil extract for 40 min sterilization and 0 min sterilization. This study have demonstrated the potential of UPLC-ESI-MS/MS to identify, characterize and profile the metabolites in heat treated whole palm oil extract for further research in developing health application of phytochemicals from palm oil. Asian Network for Scientific Information 2011 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/29296/1/MohamadRojiSarmidi2011_MetabolitesProfilingofHeatTreatedWhole.pdf Mohd. Fauzi, N. A. and Sarmidi, Mohamad Roji and Chua, Lee Suan (2011) Metabolites profiling of heat treated whole palm oil extract. Journal of Applied Sciences, 11 (13). pp. 2376-2381. ISSN 1812-5654 http://dx.doi.org/10.3923/jas.2011.2376.2381 DOI:10.3923/jas.2011.2376.2381
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Mohd. Fauzi, N. A.
Sarmidi, Mohamad Roji
Chua, Lee Suan
Metabolites profiling of heat treated whole palm oil extract
description The chemically complex and diverse nature of the plant metabolome require several platform technologies to profile the entire range of metabolites. An ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) technique was used to profile and identify a set of small-molecule metabolites found in heat treated whole palm oil extract. An investigation was carried out on the effect of heat treatment on the yield, quality and metabolites profile for whole palm oil extract. Palm fruits were collected, cleaned and sterilized for 0, 20, 40 and 60 min. The pulps were then stripped from the sterilized fruits and later was pressed using laboratory scale expeller. The resulting puree was centrifuged at 4000 rpm for 20 min. The result shows that there was a significantly difference between sterilization time of 0 and 40 min in yield and quality. Of all, the highest oil yield of 19.9±0.21% (w/w) was obtained at 40 min of sterilization with DOBI value of 5.95±0.08 and FFA of 1.44±0.22%. The MarkerView software version 1.2.0.1 analysis of the UPLC-E SI -MS/MS preliminary experimental data demonstrated the distribution and identity of several compounds in the whole palm oil extract for 40 min sterilization and 0 min sterilization. This study have demonstrated the potential of UPLC-ESI-MS/MS to identify, characterize and profile the metabolites in heat treated whole palm oil extract for further research in developing health application of phytochemicals from palm oil.
format Article
author Mohd. Fauzi, N. A.
Sarmidi, Mohamad Roji
Chua, Lee Suan
author_facet Mohd. Fauzi, N. A.
Sarmidi, Mohamad Roji
Chua, Lee Suan
author_sort Mohd. Fauzi, N. A.
title Metabolites profiling of heat treated whole palm oil extract
title_short Metabolites profiling of heat treated whole palm oil extract
title_full Metabolites profiling of heat treated whole palm oil extract
title_fullStr Metabolites profiling of heat treated whole palm oil extract
title_full_unstemmed Metabolites profiling of heat treated whole palm oil extract
title_sort metabolites profiling of heat treated whole palm oil extract
publisher Asian Network for Scientific Information
publishDate 2011
url http://eprints.utm.my/id/eprint/29296/1/MohamadRojiSarmidi2011_MetabolitesProfilingofHeatTreatedWhole.pdf
http://eprints.utm.my/id/eprint/29296/
http://dx.doi.org/10.3923/jas.2011.2376.2381
_version_ 1681489437147529216
score 13.211869

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