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QCM-D study of ß-casein on silicate-PEG surfaces

Nonspecific protein adsorption generally occurs on any solid surfaces and usually has adverse consequences. Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. Surfaces modified with end-tethered poly(ethylene glycol) (PEG) have been shown to...

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Main Authors: Ngadi, Norzita, Abrahamson, John, Fee, Conan, Morison, Ken
Format: Article
Language:English
Published: Asian Network for Scientific Information 2010
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/26575/1/NorzitaNgadi2010_QCM-DStudyOf%C3%9F-caseinOnSilicate-PEGSurfaces.pdf
http://eprints.utm.my/id/eprint/26575/
http://dx.doi.org/10.3923/jas.2010.3343.3348
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spelling my.utm.265752017-10-13T12:43:35Z http://eprints.utm.my/id/eprint/26575/ QCM-D study of ß-casein on silicate-PEG surfaces Ngadi, Norzita Abrahamson, John Fee, Conan Morison, Ken QD Chemistry Nonspecific protein adsorption generally occurs on any solid surfaces and usually has adverse consequences. Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. Surfaces modified with end-tethered poly(ethylene glycol) (PEG) have been shown to be protein-resistant to some degree. In this study, the adsorption of ß-casein was performed on several surfaces where PEG was tethered onto stainless steel by silicate. Protein adsorption was also performed on the bare stainless steel (SS) surface for comparison. The adsorption was conducted at 23 and 40°C and pH 7.2. In situ Quartz Crystal Microbalance/Dissipation (QCM-D) was used to determine PEG adsorption kinetics, plateau PEG chain densities, protein adsorption kinetics and plateau protein adsorbed quantities. The results showed that the presence of PEG molecules reduced the adsorption of ß-casein, more than 50% than that on the bare SS surface. Interestingly, the adsorption of ß-casein on the PEG surfaces at 40°C was much lower than that at 23°C. Asian Network for Scientific Information 2010-12 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/26575/1/NorzitaNgadi2010_QCM-DStudyOf%C3%9F-caseinOnSilicate-PEGSurfaces.pdf Ngadi, Norzita and Abrahamson, John and Fee, Conan and Morison, Ken (2010) QCM-D study of ß-casein on silicate-PEG surfaces. Journal of Applied Sciences, 10 (24). 3343 -3348. ISSN 1812-5654 http://dx.doi.org/10.3923/jas.2010.3343.3348 DOI:10.3923/jas.2010.3343.3348
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ngadi, Norzita
Abrahamson, John
Fee, Conan
Morison, Ken
QCM-D study of ß-casein on silicate-PEG surfaces
description Nonspecific protein adsorption generally occurs on any solid surfaces and usually has adverse consequences. Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. Surfaces modified with end-tethered poly(ethylene glycol) (PEG) have been shown to be protein-resistant to some degree. In this study, the adsorption of ß-casein was performed on several surfaces where PEG was tethered onto stainless steel by silicate. Protein adsorption was also performed on the bare stainless steel (SS) surface for comparison. The adsorption was conducted at 23 and 40°C and pH 7.2. In situ Quartz Crystal Microbalance/Dissipation (QCM-D) was used to determine PEG adsorption kinetics, plateau PEG chain densities, protein adsorption kinetics and plateau protein adsorbed quantities. The results showed that the presence of PEG molecules reduced the adsorption of ß-casein, more than 50% than that on the bare SS surface. Interestingly, the adsorption of ß-casein on the PEG surfaces at 40°C was much lower than that at 23°C.
format Article
author Ngadi, Norzita
Abrahamson, John
Fee, Conan
Morison, Ken
author_facet Ngadi, Norzita
Abrahamson, John
Fee, Conan
Morison, Ken
author_sort Ngadi, Norzita
title QCM-D study of ß-casein on silicate-PEG surfaces
title_short QCM-D study of ß-casein on silicate-PEG surfaces
title_full QCM-D study of ß-casein on silicate-PEG surfaces
title_fullStr QCM-D study of ß-casein on silicate-PEG surfaces
title_full_unstemmed QCM-D study of ß-casein on silicate-PEG surfaces
title_sort qcm-d study of ß-casein on silicate-peg surfaces
publisher Asian Network for Scientific Information
publishDate 2010
url http://eprints.utm.my/id/eprint/26575/1/NorzitaNgadi2010_QCM-DStudyOf%C3%9F-caseinOnSilicate-PEGSurfaces.pdf
http://eprints.utm.my/id/eprint/26575/
http://dx.doi.org/10.3923/jas.2010.3343.3348
_version_ 1643647799075536896
score 13.211869

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