Staff View: Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1

Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1

This paper presents results on the effect of fermentation variables on CGTase production from Bacillus stearothermophilus HR1. Initial screening of temperature, pH and agitation speed was determined using a 2-level full factorial design. In this study, we have improved the production of cyclodextrin...

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Main Authors:	Wong, T. E., Md. Illias, Rosli, Md. Salleh, Madihah, Hassan, Osman, Kamaruddin, Kamarulzaman, Abd. Rahman, Roshanida
Format:	Article
Language:	English
Published:	Curtin University of Technology 2005
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/207/1/ks-proceedingSomCheMAL140.doc http://eprints.utm.my/id/eprint/207/ http://dx.doi.org/10.1002/apj.5500130504
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spelling	my.utm.2072017-10-23T06:27:29Z http://eprints.utm.my/id/eprint/207/ Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1 Wong, T. E. Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abd. Rahman, Roshanida TP Chemical technology This paper presents results on the effect of fermentation variables on CGTase production from Bacillus stearothermophilus HR1. Initial screening of temperature, pH and agitation speed was determined using a 2-level full factorial design. In this study, we have improved the production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in flasks using the experimental design approach. The regression model generated for the correlation of CGTase with the variables was 99.97% accurate. Results of the screening process indicated that pH and agitation speed were of greater significance compared to temperature. Results suggested that only two variables, namely pH and agitation speed, should be considered in future optimization studies. Temperature was least significant and it was set at 55d̀C where CGTase production of 10.41 U/ml was slightly higher compared to temperatures of 37d̀C, 45d̀C, 50d̀C and 60d̀C. Curtin University of Technology 2005-05 Article PeerReviewed application/msword en http://eprints.utm.my/id/eprint/207/1/ks-proceedingSomCheMAL140.doc Wong, T. E. and Md. Illias, Rosli and Md. Salleh, Madihah and Hassan, Osman and Kamaruddin, Kamarulzaman and Abd. Rahman, Roshanida (2005) Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1. Developments in Chemical Engineering and Mineral Processing, 13 (5-6). pp. 541-547. ISSN 1932-2143 (electronic) http://dx.doi.org/10.1002/apj.5500130504 doi:10.1002/apj.5500130504
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Wong, T. E. Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abd. Rahman, Roshanida Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
description	This paper presents results on the effect of fermentation variables on CGTase production from Bacillus stearothermophilus HR1. Initial screening of temperature, pH and agitation speed was determined using a 2-level full factorial design. In this study, we have improved the production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in flasks using the experimental design approach. The regression model generated for the correlation of CGTase with the variables was 99.97% accurate. Results of the screening process indicated that pH and agitation speed were of greater significance compared to temperature. Results suggested that only two variables, namely pH and agitation speed, should be considered in future optimization studies. Temperature was least significant and it was set at 55d̀C where CGTase production of 10.41 U/ml was slightly higher compared to temperatures of 37d̀C, 45d̀C, 50d̀C and 60d̀C.
format	Article
author	Wong, T. E. Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abd. Rahman, Roshanida
author_facet	Wong, T. E. Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abd. Rahman, Roshanida
author_sort	Wong, T. E.
title	Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
title_short	Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
title_full	Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
title_fullStr	Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
title_full_unstemmed	Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
title_sort	initial screening of fermentation variables for the production of novel cyclomaltodextrin glucanotransferase (cgtase) from local isolated bacillus stearothermophilus hr1
publisher	Curtin University of Technology
publishDate	2005
url	http://eprints.utm.my/id/eprint/207/1/ks-proceedingSomCheMAL140.doc http://eprints.utm.my/id/eprint/207/ http://dx.doi.org/10.1002/apj.5500130504
_version_	1643643041861337088
score	13.211869

Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1

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