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Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study

Cholesterol detection has much importance and researchers have developed many kinds of biosensors but still the lack of a rapid, cheap and efficient biosensor is clearly visible. In this study, Meso-structured Cellular Foam (MCF) materials have been investigated as a support for the immobilization o...

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Main Authors: Mohd. Yusof, Alias, Mohd. Yusof, Abdull Rahim, Shahir, Shafinaz, Mohammad Arif, Shakil
Format: Conference or Workshop Item
Published: 2009
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/15282/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99365
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spelling my.utm.152822020-08-30T08:46:18Z http://eprints.utm.my/id/eprint/15282/ Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study Mohd. Yusof, Alias Mohd. Yusof, Abdull Rahim Shahir, Shafinaz Mohammad Arif, Shakil Q Science (General) Cholesterol detection has much importance and researchers have developed many kinds of biosensors but still the lack of a rapid, cheap and efficient biosensor is clearly visible. In this study, Meso-structured Cellular Foam (MCF) materials have been investigated as a support for the immobilization of cholesterol oxidase. For this purpose, MCF was synthesized, characterized and modified with amino groups using amino-alkoxysilane. The amino functionalized MCF was further modified by glutaraldehyde. The characteristic pore structure of MCF was confirmed with X-ray diffraction (XRD) while the surface area and pore width were determined with surface area analyzer using nitrogen adsorption-desorption technique. The morphology of MCF was investigated using Field Emission Scanning Electron Microscopy (FESEM) while functional groups were detected by Fourier Transform Infrared Spectrophotometry (FTIR). Both amino and glutaraldehyde modified MCF were used for the immobilization of cholesterol oxidase. The activity of free and immobilized enzyme were determined by continuous spectrometric rate determination method. The activity of free enzyme was 0.06170 unit/mL and that of residue solution was 0.00240 and 0.00016 unit/mL for glutaraldehyde and amino functionalized MCF, respectively. The very low absorbance of residue solutions compare to that of free enzyme indicates that the immobilization was successful. So modified MCF can to be a good support for enzymes and bulky bio-molecules. 2009 Conference or Workshop Item PeerReviewed Mohd. Yusof, Alias and Mohd. Yusof, Abdull Rahim and Shahir, Shafinaz and Mohammad Arif, Shakil (2009) Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study. In: 2nd International Conference and workshops on Basic and Applied Sciences, 2009, Johor Bahru. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99365
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Mohd. Yusof, Alias
Mohd. Yusof, Abdull Rahim
Shahir, Shafinaz
Mohammad Arif, Shakil
Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
description Cholesterol detection has much importance and researchers have developed many kinds of biosensors but still the lack of a rapid, cheap and efficient biosensor is clearly visible. In this study, Meso-structured Cellular Foam (MCF) materials have been investigated as a support for the immobilization of cholesterol oxidase. For this purpose, MCF was synthesized, characterized and modified with amino groups using amino-alkoxysilane. The amino functionalized MCF was further modified by glutaraldehyde. The characteristic pore structure of MCF was confirmed with X-ray diffraction (XRD) while the surface area and pore width were determined with surface area analyzer using nitrogen adsorption-desorption technique. The morphology of MCF was investigated using Field Emission Scanning Electron Microscopy (FESEM) while functional groups were detected by Fourier Transform Infrared Spectrophotometry (FTIR). Both amino and glutaraldehyde modified MCF were used for the immobilization of cholesterol oxidase. The activity of free and immobilized enzyme were determined by continuous spectrometric rate determination method. The activity of free enzyme was 0.06170 unit/mL and that of residue solution was 0.00240 and 0.00016 unit/mL for glutaraldehyde and amino functionalized MCF, respectively. The very low absorbance of residue solutions compare to that of free enzyme indicates that the immobilization was successful. So modified MCF can to be a good support for enzymes and bulky bio-molecules.
format Conference or Workshop Item
author Mohd. Yusof, Alias
Mohd. Yusof, Abdull Rahim
Shahir, Shafinaz
Mohammad Arif, Shakil
author_facet Mohd. Yusof, Alias
Mohd. Yusof, Abdull Rahim
Shahir, Shafinaz
Mohammad Arif, Shakil
author_sort Mohd. Yusof, Alias
title Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
title_short Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
title_full Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
title_fullStr Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
title_full_unstemmed Immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
title_sort immobilization of cholesterol oxidase in ordered mesoporous silica based materials and its activity study
publishDate 2009
url http://eprints.utm.my/id/eprint/15282/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99365
_version_ 1677781062936166400
score 13.211869

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