Cover Image

Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques

Combination of alkali-microwave pre-treatment on empty fruit bunches (EFB) fibre changed the morphology and properties of the EFB fibre as observed through scanning electron microscope (SEM) and Fourier transformed infrared spectroscopy (FT-IR). Pre-treatment process raptures the lignin and hemicell...

Full description

Saved in:
Bibliographic Details
Main Authors: Idris, Ani, Hamzah, Fazlena
Format: Article
Language:English
Published: Elsevier Ltd. 2008
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/13914/1/AinIdris2008_EnzymaticHydrolysisofTreatedPalmOilEmptyFruitBunches.pdf
http://eprints.utm.my/id/eprint/13914/
https://dx.doi.org/10.1016/j.jbiotec.2008.07.938
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.13914
record_format eprints
spelling my.utm.139142018-11-29T07:34:42Z http://eprints.utm.my/id/eprint/13914/ Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques Idris, Ani Hamzah, Fazlena TP Chemical technology Combination of alkali-microwave pre-treatment on empty fruit bunches (EFB) fibre changed the morphology and properties of the EFB fibre as observed through scanning electron microscope (SEM) and Fourier transformed infrared spectroscopy (FT-IR). Pre-treatment process raptures the lignin and hemicellulose component inside the fibre, thus leaving available cellulose for hydrolysis process (Vlasenko et al., 1996). Most of the silica component and any impurities on the surface of the raw EFB fibre were removed during pre-treatment process, leaving an empty cauldron on the treated EFB surface. Furthermore, an internal structure of pre-treated EFB fibre showed a clear macrofibril compared to the untreated EFB fibre. The microfibrils of pre-treated fibre were separated from the initial connected structure and this exposed the cellulose to hydrolysis, thus increased the external surface area and the porosity of the pre-treated fibre (Xu et al., 2007). Generally, raw EFB fibre consists of 44.2% alpha cellulose, 33.5% hemicellulose and 20.4% lignin, respectively (Astima et al., 2002). After treating with alkali-microwave, cellulose composition increased to 64%, while hemicellulose and lignin composition reduced to 26% and 8%, respectively. Cellulose composition is also much higher in microwave treatment compared with conventional pre-treatment. Alkali-microwave pre-treated EFB fibre gave 30% soluble glucose higher than conventional pre-treatment when it was hydrolyzed with combination of cellulase and Novozyme 188. The optimum conditions obtained for hydrolysis process were at pH 5, 50 °C and 5:1 cellulase to Novozyme 188 ratio. Elsevier Ltd. 2008 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/13914/1/AinIdris2008_EnzymaticHydrolysisofTreatedPalmOilEmptyFruitBunches.pdf Idris, Ani and Hamzah, Fazlena (2008) Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques. Journal of Biotechnology, 136 (Supp 1). S406. ISSN 0168-1656 https://dx.doi.org/10.1016/j.jbiotec.2008.07.938 DOI:10.1016/j.jbiotec.2008.07.938
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Idris, Ani
Hamzah, Fazlena
Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
description Combination of alkali-microwave pre-treatment on empty fruit bunches (EFB) fibre changed the morphology and properties of the EFB fibre as observed through scanning electron microscope (SEM) and Fourier transformed infrared spectroscopy (FT-IR). Pre-treatment process raptures the lignin and hemicellulose component inside the fibre, thus leaving available cellulose for hydrolysis process (Vlasenko et al., 1996). Most of the silica component and any impurities on the surface of the raw EFB fibre were removed during pre-treatment process, leaving an empty cauldron on the treated EFB surface. Furthermore, an internal structure of pre-treated EFB fibre showed a clear macrofibril compared to the untreated EFB fibre. The microfibrils of pre-treated fibre were separated from the initial connected structure and this exposed the cellulose to hydrolysis, thus increased the external surface area and the porosity of the pre-treated fibre (Xu et al., 2007). Generally, raw EFB fibre consists of 44.2% alpha cellulose, 33.5% hemicellulose and 20.4% lignin, respectively (Astima et al., 2002). After treating with alkali-microwave, cellulose composition increased to 64%, while hemicellulose and lignin composition reduced to 26% and 8%, respectively. Cellulose composition is also much higher in microwave treatment compared with conventional pre-treatment. Alkali-microwave pre-treated EFB fibre gave 30% soluble glucose higher than conventional pre-treatment when it was hydrolyzed with combination of cellulase and Novozyme 188. The optimum conditions obtained for hydrolysis process were at pH 5, 50 °C and 5:1 cellulase to Novozyme 188 ratio.
format Article
author Idris, Ani
Hamzah, Fazlena
author_facet Idris, Ani
Hamzah, Fazlena
author_sort Idris, Ani
title Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
title_short Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
title_full Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
title_fullStr Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
title_full_unstemmed Enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
title_sort enzymatic hydrolysis of treated palm oil empty fruit bunches fibre (efb) using combination alkali-microwave techniques
publisher Elsevier Ltd.
publishDate 2008
url http://eprints.utm.my/id/eprint/13914/1/AinIdris2008_EnzymaticHydrolysisofTreatedPalmOilEmptyFruitBunches.pdf
http://eprints.utm.my/id/eprint/13914/
https://dx.doi.org/10.1016/j.jbiotec.2008.07.938
_version_ 1643646292490977280
score 13.211869

Similar Items