Staff View: Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)

Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)

Heavy industrial activities and agricultural processes require consumption of many halogenated compounds, and release them continuously as pollutants into the environment. These xenobiotics show high toxicity and persistence and cause many problems to the society, soils and ground water. Microbial d...

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Main Authors:	Jing, Ng Hong, Mohd. Taha, Aishah, Pakingking, Rolando V., Wahab, Roswanira A. B., Huyop, Fahrul
Format:	Article
Published:	Academic Journals 2008
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/12829/
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id	my.utm.12829
record_format	eprints
spelling	my.utm.128292017-10-25T06:14:29Z http://eprints.utm.my/id/eprint/12829/ Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon) Jing, Ng Hong Mohd. Taha, Aishah Pakingking, Rolando V. Wahab, Roswanira A. B. Huyop, Fahrul QD Chemistry Heavy industrial activities and agricultural processes require consumption of many halogenated compounds, and release them continuously as pollutants into the environment. These xenobiotics show high toxicity and persistence and cause many problems to the society, soils and ground water. Microbial dehalogenases are involved in the biodegradation of many important chlorinated compounds. A bacterial strain identified as Methylobacterium sp. HJ1 is able to degrade the herbicide 2,2-dichloropropionic acid by removal of the halogen and subsequent metabolism of the product for energy. D, L-2-chloropropionate also supported good growth of the organism but 3-chloropropionate, monochloroacetate and dichloroacetate were not utilized. Cell-free extracts of the 2,2-dichloropropionate-grown bacteria converted 2,2-dichloropropionate into pyruvate with the release of two chloride ions for each molecule of pyruvate formed. This indicates the presence of dehalogenase activity in the cell-free extracts. Only 2,2-dichloropropionate and D, L-2-chloropropionate were inducers and substrates for the dehalogenase. Monochloroacetate and dichloroacetate did not serve as an inducer, whereas 3-chloropropionate was a non-substrate inducer. Academic Journals 2008-02 Article PeerReviewed Jing, Ng Hong and Mohd. Taha, Aishah and Pakingking, Rolando V. and Wahab, Roswanira A. B. and Huyop, Fahrul (2008) Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon). African Journal Of Microbiology Research, 2 (2). pp. 32-36. ISSN 1996-0808
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	QD Chemistry
spellingShingle	QD Chemistry Jing, Ng Hong Mohd. Taha, Aishah Pakingking, Rolando V. Wahab, Roswanira A. B. Huyop, Fahrul Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
description	Heavy industrial activities and agricultural processes require consumption of many halogenated compounds, and release them continuously as pollutants into the environment. These xenobiotics show high toxicity and persistence and cause many problems to the society, soils and ground water. Microbial dehalogenases are involved in the biodegradation of many important chlorinated compounds. A bacterial strain identified as Methylobacterium sp. HJ1 is able to degrade the herbicide 2,2-dichloropropionic acid by removal of the halogen and subsequent metabolism of the product for energy. D, L-2-chloropropionate also supported good growth of the organism but 3-chloropropionate, monochloroacetate and dichloroacetate were not utilized. Cell-free extracts of the 2,2-dichloropropionate-grown bacteria converted 2,2-dichloropropionate into pyruvate with the release of two chloride ions for each molecule of pyruvate formed. This indicates the presence of dehalogenase activity in the cell-free extracts. Only 2,2-dichloropropionate and D, L-2-chloropropionate were inducers and substrates for the dehalogenase. Monochloroacetate and dichloroacetate did not serve as an inducer, whereas 3-chloropropionate was a non-substrate inducer.
format	Article
author	Jing, Ng Hong Mohd. Taha, Aishah Pakingking, Rolando V. Wahab, Roswanira A. B. Huyop, Fahrul
author_facet	Jing, Ng Hong Mohd. Taha, Aishah Pakingking, Rolando V. Wahab, Roswanira A. B. Huyop, Fahrul
author_sort	Jing, Ng Hong
title	Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
title_short	Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
title_full	Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
title_fullStr	Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
title_full_unstemmed	Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)
title_sort	dehalogenase from methylobacterium sp hj1 induced by the herbicide 2, 2-dichloropropionate (dalapon)
publisher	Academic Journals
publishDate	2008
url	http://eprints.utm.my/id/eprint/12829/
_version_	1643646051240902656
score	13.211869

Dehalogenase from methylobacterium sp HJ1 induced by the herbicide 2, 2-dichloropropionate (Dalapon)

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