Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering

Tissue engineering is currently one of the fastest-growing areas of engineering, requiring the fabrication of advanced and multifunctional materials that can be used as scaffolds or dressings for tissue regeneration. In this work, we report a bilayer material prepared by electrospinning a hybrid mat...

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Main Authors: Khan, Rawaiz, Aslam Khan, Muhammad Umar, Stojanović, Goran M., Javed, Aneela, Haider, Sajjad, Abd. Razak, Saiful Izwan
Format: Article
Language:English
Published: American Chemical Society 2024
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Online Access:http://eprints.utm.my/108750/1/SaifulIzwan2024_FabricationofBilayerNanofibrousHydrogelScaffold.pdf
http://eprints.utm.my/108750/
http://dx.doi.org/10.1021/acsomega.3c06613
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spelling my.utm.1087502024-12-11T09:25:43Z http://eprints.utm.my/108750/ Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering Khan, Rawaiz Aslam Khan, Muhammad Umar Stojanović, Goran M. Javed, Aneela Haider, Sajjad Abd. Razak, Saiful Izwan TP Chemical technology Tissue engineering is currently one of the fastest-growing areas of engineering, requiring the fabrication of advanced and multifunctional materials that can be used as scaffolds or dressings for tissue regeneration. In this work, we report a bilayer material prepared by electrospinning a hybrid material of poly(vinyl alcohol) (PVA) and bacterial cellulose (BC NFs) (top layer) over a highly interconnected porous 3D gelatin-PVA hydrogel obtained by a freeze-drying process (bottom layer). The techniques were combined to produce an advanced material with synergistic effects on the physical and biological properties of the two materials. The bilayer material was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and a water contact measurement system (WCMS). Studies on swelling, degradability, porosity, drug release, cellular and antibacterial activities were performed using standardized procedures and assays. FTIR confirmed cross-linking of both the top and bottom layers, and SEM showed porous structure for the bottom layer, random deposition of NFs on the surface, and aligned NFs in the cross section. The water contact angle (WCA) showed a hydrophilic surface for the bilayer material. Swelling analysis showed high swelling, and degradation analysis showed good stability. The bilayer material released Ag-sulfadiazine in a sustained and controlled manner and showed good antibacterial activities against severe disease-causing gram + ive and −ive (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) bacterial strains. In vitro biological studies were performed on fibroblasts (3T3) and human embryonic kidneys (HEK-293), which showed desirable cell viability, proliferation, and adhesion to the bilayer. Thus, the synergistic effect of NFs and the hydrogel resulted in a potential wound dressing material for wound healing and soft tissue engineering. American Chemical Society 2024 Article PeerReviewed application/pdf en http://eprints.utm.my/108750/1/SaifulIzwan2024_FabricationofBilayerNanofibrousHydrogelScaffold.pdf Khan, Rawaiz and Aslam Khan, Muhammad Umar and Stojanović, Goran M. and Javed, Aneela and Haider, Sajjad and Abd. Razak, Saiful Izwan (2024) Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering. ACS Omega, 9 (6). pp. 6527-6536. ISSN 2470-1343 http://dx.doi.org/10.1021/acsomega.3c06613 DOI : 10.1021/acsomega.3c06613
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Khan, Rawaiz
Aslam Khan, Muhammad Umar
Stojanović, Goran M.
Javed, Aneela
Haider, Sajjad
Abd. Razak, Saiful Izwan
Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
description Tissue engineering is currently one of the fastest-growing areas of engineering, requiring the fabrication of advanced and multifunctional materials that can be used as scaffolds or dressings for tissue regeneration. In this work, we report a bilayer material prepared by electrospinning a hybrid material of poly(vinyl alcohol) (PVA) and bacterial cellulose (BC NFs) (top layer) over a highly interconnected porous 3D gelatin-PVA hydrogel obtained by a freeze-drying process (bottom layer). The techniques were combined to produce an advanced material with synergistic effects on the physical and biological properties of the two materials. The bilayer material was characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and a water contact measurement system (WCMS). Studies on swelling, degradability, porosity, drug release, cellular and antibacterial activities were performed using standardized procedures and assays. FTIR confirmed cross-linking of both the top and bottom layers, and SEM showed porous structure for the bottom layer, random deposition of NFs on the surface, and aligned NFs in the cross section. The water contact angle (WCA) showed a hydrophilic surface for the bilayer material. Swelling analysis showed high swelling, and degradation analysis showed good stability. The bilayer material released Ag-sulfadiazine in a sustained and controlled manner and showed good antibacterial activities against severe disease-causing gram + ive and −ive (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) bacterial strains. In vitro biological studies were performed on fibroblasts (3T3) and human embryonic kidneys (HEK-293), which showed desirable cell viability, proliferation, and adhesion to the bilayer. Thus, the synergistic effect of NFs and the hydrogel resulted in a potential wound dressing material for wound healing and soft tissue engineering.
format Article
author Khan, Rawaiz
Aslam Khan, Muhammad Umar
Stojanović, Goran M.
Javed, Aneela
Haider, Sajjad
Abd. Razak, Saiful Izwan
author_facet Khan, Rawaiz
Aslam Khan, Muhammad Umar
Stojanović, Goran M.
Javed, Aneela
Haider, Sajjad
Abd. Razak, Saiful Izwan
author_sort Khan, Rawaiz
title Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
title_short Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
title_full Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
title_fullStr Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
title_full_unstemmed Fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, PVA, and gelatin as advanced dressing for wound healing and soft tissue engineering
title_sort fabrication of bilayer nanofibrous-hydrogel scaffold from bacterial cellulose, pva, and gelatin as advanced dressing for wound healing and soft tissue engineering
publisher American Chemical Society
publishDate 2024
url http://eprints.utm.my/108750/1/SaifulIzwan2024_FabricationofBilayerNanofibrousHydrogelScaffold.pdf
http://eprints.utm.my/108750/
http://dx.doi.org/10.1021/acsomega.3c06613
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