Application of fish flour liquid waste as alternative growth media for producing xylanase
Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to...
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my.utm.1079002024-10-08T06:58:35Z http://eprints.utm.my/107900/ Application of fish flour liquid waste as alternative growth media for producing xylanase Budiman, Frederick Nurrohman, Ardiana Ilham Ekwanda, Refka Revina Melyata Ginarto Arif Wicaksono, Ginarto Arif Wicaksono Khairun Nisa, Khairun Nisa Yamani, Laura Navika Md. Ilias, Rosli Tri Puspaningsih, Ni Nyoman TP Chemical technology Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5a harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively. 2023 Conference or Workshop Item PeerReviewed Budiman, Frederick and Nurrohman, Ardiana Ilham and Ekwanda, Refka Revina Melyata and Ginarto Arif Wicaksono, Ginarto Arif Wicaksono and Khairun Nisa, Khairun Nisa and Yamani, Laura Navika and Md. Ilias, Rosli and Tri Puspaningsih, Ni Nyoman (2023) Application of fish flour liquid waste as alternative growth media for producing xylanase. In: 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021, 18 September 2021-19 September 2021, Jember, Indonesia. http://dx.doi.org/10.1063/5.0111694 |
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TP Chemical technology Budiman, Frederick Nurrohman, Ardiana Ilham Ekwanda, Refka Revina Melyata Ginarto Arif Wicaksono, Ginarto Arif Wicaksono Khairun Nisa, Khairun Nisa Yamani, Laura Navika Md. Ilias, Rosli Tri Puspaningsih, Ni Nyoman Application of fish flour liquid waste as alternative growth media for producing xylanase |
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Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5a harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively. |
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Conference or Workshop Item |
author |
Budiman, Frederick Nurrohman, Ardiana Ilham Ekwanda, Refka Revina Melyata Ginarto Arif Wicaksono, Ginarto Arif Wicaksono Khairun Nisa, Khairun Nisa Yamani, Laura Navika Md. Ilias, Rosli Tri Puspaningsih, Ni Nyoman |
author_facet |
Budiman, Frederick Nurrohman, Ardiana Ilham Ekwanda, Refka Revina Melyata Ginarto Arif Wicaksono, Ginarto Arif Wicaksono Khairun Nisa, Khairun Nisa Yamani, Laura Navika Md. Ilias, Rosli Tri Puspaningsih, Ni Nyoman |
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Budiman, Frederick |
title |
Application of fish flour liquid waste as alternative growth media for producing xylanase |
title_short |
Application of fish flour liquid waste as alternative growth media for producing xylanase |
title_full |
Application of fish flour liquid waste as alternative growth media for producing xylanase |
title_fullStr |
Application of fish flour liquid waste as alternative growth media for producing xylanase |
title_full_unstemmed |
Application of fish flour liquid waste as alternative growth media for producing xylanase |
title_sort |
application of fish flour liquid waste as alternative growth media for producing xylanase |
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2023 |
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http://eprints.utm.my/107900/ http://dx.doi.org/10.1063/5.0111694 |
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1814043553902362624 |
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13.211869 |