Application of fish flour liquid waste as alternative growth media for producing xylanase

Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to...

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Main Authors: Budiman, Frederick, Nurrohman, Ardiana Ilham, Ekwanda, Refka Revina Melyata, Ginarto Arif Wicaksono, Ginarto Arif Wicaksono, Khairun Nisa, Khairun Nisa, Yamani, Laura Navika, Md. Ilias, Rosli, Tri Puspaningsih, Ni Nyoman
Format: Conference or Workshop Item
Published: 2023
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Online Access:http://eprints.utm.my/107900/
http://dx.doi.org/10.1063/5.0111694
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spelling my.utm.1079002024-10-08T06:58:35Z http://eprints.utm.my/107900/ Application of fish flour liquid waste as alternative growth media for producing xylanase Budiman, Frederick Nurrohman, Ardiana Ilham Ekwanda, Refka Revina Melyata Ginarto Arif Wicaksono, Ginarto Arif Wicaksono Khairun Nisa, Khairun Nisa Yamani, Laura Navika Md. Ilias, Rosli Tri Puspaningsih, Ni Nyoman TP Chemical technology Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5a harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively. 2023 Conference or Workshop Item PeerReviewed Budiman, Frederick and Nurrohman, Ardiana Ilham and Ekwanda, Refka Revina Melyata and Ginarto Arif Wicaksono, Ginarto Arif Wicaksono and Khairun Nisa, Khairun Nisa and Yamani, Laura Navika and Md. Ilias, Rosli and Tri Puspaningsih, Ni Nyoman (2023) Application of fish flour liquid waste as alternative growth media for producing xylanase. In: 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021, 18 September 2021-19 September 2021, Jember, Indonesia. http://dx.doi.org/10.1063/5.0111694
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Budiman, Frederick
Nurrohman, Ardiana Ilham
Ekwanda, Refka Revina Melyata
Ginarto Arif Wicaksono, Ginarto Arif Wicaksono
Khairun Nisa, Khairun Nisa
Yamani, Laura Navika
Md. Ilias, Rosli
Tri Puspaningsih, Ni Nyoman
Application of fish flour liquid waste as alternative growth media for producing xylanase
description Consumption of processed fish product increase year by year, especially fish flour. However, processing fish flour produce untreated wastewater that contains rich element of carbon, nitrogen, and mineral. Those rich elements can be used as alternative bacterial nutrient media. This research aims to devise a combine formulation between fish flour liquid waste and Luria Bertani media. The combine medium was used to grow recombinant Eschericia coli DH5a harboring gene encoding xylanolytic enzyme of pTP510 with ratio of combine medium 1:2, 1:2, and 1:4 compared to Luria Bertani media itself. The result showed that the optimum combine formulation was 1:2 and indicated its activity of 0.7260 Unit/mL and 0.6213 Unit/mL after 9 hours and 12 hours incubation time, respectively.
format Conference or Workshop Item
author Budiman, Frederick
Nurrohman, Ardiana Ilham
Ekwanda, Refka Revina Melyata
Ginarto Arif Wicaksono, Ginarto Arif Wicaksono
Khairun Nisa, Khairun Nisa
Yamani, Laura Navika
Md. Ilias, Rosli
Tri Puspaningsih, Ni Nyoman
author_facet Budiman, Frederick
Nurrohman, Ardiana Ilham
Ekwanda, Refka Revina Melyata
Ginarto Arif Wicaksono, Ginarto Arif Wicaksono
Khairun Nisa, Khairun Nisa
Yamani, Laura Navika
Md. Ilias, Rosli
Tri Puspaningsih, Ni Nyoman
author_sort Budiman, Frederick
title Application of fish flour liquid waste as alternative growth media for producing xylanase
title_short Application of fish flour liquid waste as alternative growth media for producing xylanase
title_full Application of fish flour liquid waste as alternative growth media for producing xylanase
title_fullStr Application of fish flour liquid waste as alternative growth media for producing xylanase
title_full_unstemmed Application of fish flour liquid waste as alternative growth media for producing xylanase
title_sort application of fish flour liquid waste as alternative growth media for producing xylanase
publishDate 2023
url http://eprints.utm.my/107900/
http://dx.doi.org/10.1063/5.0111694
_version_ 1814043553902362624
score 13.211869