Staff View: Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.

Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.

Natural antioxidants have gained a huge amount of interest due to their ability to combat devastating ailments such as obesity and hypercholesterolemia. Rich in phenolics and flavonoids, Moringa Oleifera (MO) has piqued the interest of many researchers. MO plants have numerous nutritional and therap...

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Main Authors:	Marimuthu, Monisha Devi Elan Solan, Othman, Rahimah, Mohd. Bohari, Siti Pauliena, Ooi, Wei Jinn
Format:	Conference or Workshop Item
Published:	2023
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://eprints.utm.my/107844/ http://dx.doi.org/10.1007/978-981-99-1695-5_31
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spelling	my.utm.1078442024-10-08T06:17:02Z http://eprints.utm.my/107844/ Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization. Marimuthu, Monisha Devi Elan Solan Othman, Rahimah Mohd. Bohari, Siti Pauliena Ooi, Wei Jinn Q Science (General) QD Chemistry Natural antioxidants have gained a huge amount of interest due to their ability to combat devastating ailments such as obesity and hypercholesterolemia. Rich in phenolics and flavonoids, Moringa Oleifera (MO) has piqued the interest of many researchers. MO plants have numerous nutritional and therapeutic advantages. The current study aims to maximize yield by optimizing the conditions of MO leaves extraction with the help of a few organic second solvents. Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize total phenolic content (TPC) and total flavonoid content (TFC) of MO extract. Four independent variables (A) type of second solvents, (B) solvent to second solvent ratio, (C) extraction temperature, and (D) extraction time were studied. TPC was evaluated using the Folin–Ciocalteu colorimetric technique, and extract solutions were measured at 765 nm. TFC was determined by aluminium chloride colorimetric test at wavenumber of 416 nm. The functional group of the optimized MO extract was subsequently studied using Fourier Transform Infrared Spectroscopy (FTIR). The optimization studies indicated that the optimum TPC was 313.265 µg · GAE ·mg−1 and TFC was 90.268 µg · QE · mg−1 which were achieved at formulation conditions of (A) acetone, (B) at 1:3, (C) of 100 °C, and (D) of 240 min. The most intense stretching peak of FTIR spectra was detected at 3262.79 cm−1 revealed the characteristics absorption of hydroxyl groups from phenolic content of MO extract. This simulated finding proved that the best extraction solvent elucidates that MO leaves are rich in valuable antioxidants with tremendous therapeutic potency for obesity and hypercholestrolaemia treatment. 2023-09-01 Conference or Workshop Item PeerReviewed Marimuthu, Monisha Devi Elan Solan and Othman, Rahimah and Mohd. Bohari, Siti Pauliena and Ooi, Wei Jinn (2023) Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization. In: 2nd International Conference on Biomass Utilization and Sustainable Energy, ICoBiomasSE 2022, 20 September 2022 - 21 September 2022, Arau, Perlis, Malaysia - Virtual, Online. http://dx.doi.org/10.1007/978-981-99-1695-5_31
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Marimuthu, Monisha Devi Elan Solan Othman, Rahimah Mohd. Bohari, Siti Pauliena Ooi, Wei Jinn Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
description	Natural antioxidants have gained a huge amount of interest due to their ability to combat devastating ailments such as obesity and hypercholesterolemia. Rich in phenolics and flavonoids, Moringa Oleifera (MO) has piqued the interest of many researchers. MO plants have numerous nutritional and therapeutic advantages. The current study aims to maximize yield by optimizing the conditions of MO leaves extraction with the help of a few organic second solvents. Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize total phenolic content (TPC) and total flavonoid content (TFC) of MO extract. Four independent variables (A) type of second solvents, (B) solvent to second solvent ratio, (C) extraction temperature, and (D) extraction time were studied. TPC was evaluated using the Folin–Ciocalteu colorimetric technique, and extract solutions were measured at 765 nm. TFC was determined by aluminium chloride colorimetric test at wavenumber of 416 nm. The functional group of the optimized MO extract was subsequently studied using Fourier Transform Infrared Spectroscopy (FTIR). The optimization studies indicated that the optimum TPC was 313.265 µg · GAE ·mg−1 and TFC was 90.268 µg · QE · mg−1 which were achieved at formulation conditions of (A) acetone, (B) at 1:3, (C) of 100 °C, and (D) of 240 min. The most intense stretching peak of FTIR spectra was detected at 3262.79 cm−1 revealed the characteristics absorption of hydroxyl groups from phenolic content of MO extract. This simulated finding proved that the best extraction solvent elucidates that MO leaves are rich in valuable antioxidants with tremendous therapeutic potency for obesity and hypercholestrolaemia treatment.
format	Conference or Workshop Item
author	Marimuthu, Monisha Devi Elan Solan Othman, Rahimah Mohd. Bohari, Siti Pauliena Ooi, Wei Jinn
author_facet	Marimuthu, Monisha Devi Elan Solan Othman, Rahimah Mohd. Bohari, Siti Pauliena Ooi, Wei Jinn
author_sort	Marimuthu, Monisha Devi Elan Solan
title	Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
title_short	Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
title_full	Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
title_fullStr	Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
title_full_unstemmed	Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.
title_sort	intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: optimization and characterization.
publishDate	2023
url	http://eprints.utm.my/107844/ http://dx.doi.org/10.1007/978-981-99-1695-5_31
_version_	1814043538628804608
score	13.211869

Intensification of antioxidant-rich extract from moringa oleifera leaves using different solvents: Optimization and characterization.

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