Staff View: The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.

The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.

Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fas...

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Main Authors:	Juliansyah, Dandy Akbar, Nurjayadi, Muktiningsih, Declan, Jefferson Lynford, Putri, Gladys Indira, Krisdawati, Ismaya, Azzahra, Maharanianska, Maulana, Irvan, Saamia, Vira, Saputro, Dwi Anna Oktaviani, Wiranatha, I Made, El-Enshasy, Hesham Ali
Format:	Conference or Workshop Item
Language:	English
Published:	2023
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/107804/1/HeshamAliElEnhasy2023_ThePotentialofgrxBGeneforDetectionofCSakazakiiinInfant.pdf http://eprints.utm.my/107804/ http://dx.doi.org/10.1051/e3sconf/202340004010
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id	my.utm.107804
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spelling	my.utm.1078042024-10-02T07:39:58Z http://eprints.utm.my/107804/ The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction. Juliansyah, Dandy Akbar Nurjayadi, Muktiningsih Declan, Jefferson Lynford Putri, Gladys Indira Krisdawati, Ismaya Azzahra, Maharanianska Maulana, Irvan Saamia, Vira Saputro, Dwi Anna Oktaviani Wiranatha, I Made El-Enshasy, Hesham Ali TP Chemical technology Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii in infant formula milk. This research aims to develop a method for detecting C. sakazakii bacteria using real-time PCR with high sensitivity, specificity, and accuracy. A rapid detection method using real-time PCR with the target gene grxB successfully detects the presence of C. sakazakii DNA in artificially contaminated formula milk. The results of the real-time PCR test showed that C. sakazakii DNA with a concentration of 53 ng/μL could be amplified by the grxB gene primer pair with a Ct value of 12 and a Tm value of 85.8oC. The specificity test showed that the grxB primer could differentiate between target and some non-target bacteria. The sensitivity test showed the ability of the grxB primer to detect the smallest concentration of 3,392 pg/μL with a Ct of 24,06. Based on the results obtained, it can be concluded that the grxB primer has the potential to be used as rapid detection method for C. sakazakii bacteria in infant formula using real-time PCR. 2023-07-03 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/107804/1/HeshamAliElEnhasy2023_ThePotentialofgrxBGeneforDetectionofCSakazakiiinInfant.pdf Juliansyah, Dandy Akbar and Nurjayadi, Muktiningsih and Declan, Jefferson Lynford and Putri, Gladys Indira and Krisdawati, Ismaya and Azzahra, Maharanianska and Maulana, Irvan and Saamia, Vira and Saputro, Dwi Anna Oktaviani and Wiranatha, I Made and El-Enshasy, Hesham Ali (2023) The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction. In: 1st International Conference on Sciences, Mathematics, and Education, ICoSMEd 2022, 13 October 2022 - 14 October 2022, Gorontalo, Sulawesi, Indonesia. http://dx.doi.org/10.1051/e3sconf/202340004010
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Juliansyah, Dandy Akbar Nurjayadi, Muktiningsih Declan, Jefferson Lynford Putri, Gladys Indira Krisdawati, Ismaya Azzahra, Maharanianska Maulana, Irvan Saamia, Vira Saputro, Dwi Anna Oktaviani Wiranatha, I Made El-Enshasy, Hesham Ali The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
description	Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii in infant formula milk. This research aims to develop a method for detecting C. sakazakii bacteria using real-time PCR with high sensitivity, specificity, and accuracy. A rapid detection method using real-time PCR with the target gene grxB successfully detects the presence of C. sakazakii DNA in artificially contaminated formula milk. The results of the real-time PCR test showed that C. sakazakii DNA with a concentration of 53 ng/μL could be amplified by the grxB gene primer pair with a Ct value of 12 and a Tm value of 85.8oC. The specificity test showed that the grxB primer could differentiate between target and some non-target bacteria. The sensitivity test showed the ability of the grxB primer to detect the smallest concentration of 3,392 pg/μL with a Ct of 24,06. Based on the results obtained, it can be concluded that the grxB primer has the potential to be used as rapid detection method for C. sakazakii bacteria in infant formula using real-time PCR.
format	Conference or Workshop Item
author	Juliansyah, Dandy Akbar Nurjayadi, Muktiningsih Declan, Jefferson Lynford Putri, Gladys Indira Krisdawati, Ismaya Azzahra, Maharanianska Maulana, Irvan Saamia, Vira Saputro, Dwi Anna Oktaviani Wiranatha, I Made El-Enshasy, Hesham Ali
author_facet	Juliansyah, Dandy Akbar Nurjayadi, Muktiningsih Declan, Jefferson Lynford Putri, Gladys Indira Krisdawati, Ismaya Azzahra, Maharanianska Maulana, Irvan Saamia, Vira Saputro, Dwi Anna Oktaviani Wiranatha, I Made El-Enshasy, Hesham Ali
author_sort	Juliansyah, Dandy Akbar
title	The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
title_short	The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
title_full	The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
title_fullStr	The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
title_full_unstemmed	The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.
title_sort	potential of grxb gene for detection of c. sakazakii in infant formula milk using real-time polymerase chain reaction.
publishDate	2023
url	http://eprints.utm.my/107804/1/HeshamAliElEnhasy2023_ThePotentialofgrxBGeneforDetectionofCSakazakiiinInfant.pdf http://eprints.utm.my/107804/ http://dx.doi.org/10.1051/e3sconf/202340004010
_version_	1814043526917259264
score	13.211869

The potential of grxB gene for detection of C. sakazakii in infant formula milk using real-time polymerase chain reaction.

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