Cover Image

Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production

In this study, the primary paper-mill sludge characterized as containing 51% glucan was used to optimize the enzymatic saccharification process for the production of bioethanol using a Box–Behnken design (BBD). Polyethylene glycol 4000 (PEG-4000) surfactant-assisted enzymatic saccharification of dri...

Full description

Saved in:
Bibliographic Details
Main Authors: Zambare, Vasudeo, Jacob, Samuel, Md. Din, Mohd. Fadhil, Ponraj, Mohanadoss
Format: Article
Language:English
Published: MDPI 2023
Subjects:
TA Engineering (General). Civil engineering (General)
TD Environmental technology. Sanitary engineering
Online Access:http://eprints.utm.my/107328/1/MohdFadhilMd2023_BoxBehnkenDesignBasedOptimization.pdf
http://eprints.utm.my/107328/
http://dx.doi.org/10.3390/su151310740
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.107328
record_format eprints
spelling my.utm.1073282024-09-01T07:15:01Z http://eprints.utm.my/107328/ Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production Zambare, Vasudeo Jacob, Samuel Md. Din, Mohd. Fadhil Ponraj, Mohanadoss TA Engineering (General). Civil engineering (General) TD Environmental technology. Sanitary engineering In this study, the primary paper-mill sludge characterized as containing 51% glucan was used to optimize the enzymatic saccharification process for the production of bioethanol using a Box–Behnken design (BBD). Polyethylene glycol 4000 (PEG-4000) surfactant-assisted enzymatic saccharification of dried primary sludge (DPS) showed a 12.8% improvement in saccharification efficiency. There was a statistically significant effect of solid enzyme loading and saccharification time on the enzymatic saccharification of DPS at a 95% confidence level (p < 0.05). The optimum levels of 10.4% w/w DPS solid loading, 2.03% enzyme loading (10 FPU g/DPS), and 1% (w/w DPS) PEG-4000 loading for a saccharification efficiency of 57.66% were validated experimentally and found to be non-significant with regard to the lack of fit with the predicted saccharification efficiency of 56.76%. Furthermore, Saccharomyces cerevisiae fermented the saccharified sugars into ethanol (9.35 g/L) with a sugar-to-ethanol conversion yield of 91.6% compared with the theoretical maximum. Therefore, DPS is a more suitable renewable biomass for determining the presence of fermentable sugar and for the production of ethanol. MDPI 2023-07 Article PeerReviewed application/pdf en http://eprints.utm.my/107328/1/MohdFadhilMd2023_BoxBehnkenDesignBasedOptimization.pdf Zambare, Vasudeo and Jacob, Samuel and Md. Din, Mohd. Fadhil and Ponraj, Mohanadoss (2023) Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production. Sustainability (Switzerland), 15 (13). pp. 1-15. ISSN 2071-1050 http://dx.doi.org/10.3390/su151310740 DOI:10.3390/su151310740
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TA Engineering (General). Civil engineering (General)
TD Environmental technology. Sanitary engineering
spellingShingle TA Engineering (General). Civil engineering (General)
TD Environmental technology. Sanitary engineering
Zambare, Vasudeo
Jacob, Samuel
Md. Din, Mohd. Fadhil
Ponraj, Mohanadoss
Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
description In this study, the primary paper-mill sludge characterized as containing 51% glucan was used to optimize the enzymatic saccharification process for the production of bioethanol using a Box–Behnken design (BBD). Polyethylene glycol 4000 (PEG-4000) surfactant-assisted enzymatic saccharification of dried primary sludge (DPS) showed a 12.8% improvement in saccharification efficiency. There was a statistically significant effect of solid enzyme loading and saccharification time on the enzymatic saccharification of DPS at a 95% confidence level (p < 0.05). The optimum levels of 10.4% w/w DPS solid loading, 2.03% enzyme loading (10 FPU g/DPS), and 1% (w/w DPS) PEG-4000 loading for a saccharification efficiency of 57.66% were validated experimentally and found to be non-significant with regard to the lack of fit with the predicted saccharification efficiency of 56.76%. Furthermore, Saccharomyces cerevisiae fermented the saccharified sugars into ethanol (9.35 g/L) with a sugar-to-ethanol conversion yield of 91.6% compared with the theoretical maximum. Therefore, DPS is a more suitable renewable biomass for determining the presence of fermentable sugar and for the production of ethanol.
format Article
author Zambare, Vasudeo
Jacob, Samuel
Md. Din, Mohd. Fadhil
Ponraj, Mohanadoss
author_facet Zambare, Vasudeo
Jacob, Samuel
Md. Din, Mohd. Fadhil
Ponraj, Mohanadoss
author_sort Zambare, Vasudeo
title Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
title_short Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
title_full Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
title_fullStr Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
title_full_unstemmed Box-Behnken design-based optimization of the Saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
title_sort box-behnken design-based optimization of the saccharification of primary paper-mill sludge as a renewable raw material for bioethanol production
publisher MDPI
publishDate 2023
url http://eprints.utm.my/107328/1/MohdFadhilMd2023_BoxBehnkenDesignBasedOptimization.pdf
http://eprints.utm.my/107328/
http://dx.doi.org/10.3390/su151310740
_version_ 1809136658608553984
score 13.211869

Similar Items