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Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.

The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this...

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Main Authors: Alwi, Aedrianee Reeza, Mahat, Naji Arafat, Mohd. Salleh, Faezah, Ishar, Seri Mirianti, Kamaluddin, Mohammad Rahim, A. Rashid, Mohd. Radzniwan
Format: Article
Published: John Wiley and Sons Inc. 2023
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/106682/
http://dx.doi.org/10.1111/1556-4029.15370
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spelling my.utm.1066822024-07-17T07:07:59Z http://eprints.utm.my/106682/ Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards. Alwi, Aedrianee Reeza Mahat, Naji Arafat Mohd. Salleh, Faezah Ishar, Seri Mirianti Kamaluddin, Mohammad Rahim A. Rashid, Mohd. Radzniwan QD Chemistry The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost. John Wiley and Sons Inc. 2023-11 Article PeerReviewed Alwi, Aedrianee Reeza and Mahat, Naji Arafat and Mohd. Salleh, Faezah and Ishar, Seri Mirianti and Kamaluddin, Mohammad Rahim and A. Rashid, Mohd. Radzniwan (2023) Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards. Journal of Forensic Sciences, 68 (6). pp. 2103-2115. ISSN 0022-1198 http://dx.doi.org/10.1111/1556-4029.15370 DOI:10.1111/1556-4029.15370
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Alwi, Aedrianee Reeza
Mahat, Naji Arafat
Mohd. Salleh, Faezah
Ishar, Seri Mirianti
Kamaluddin, Mohammad Rahim
A. Rashid, Mohd. Radzniwan
Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
description The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost.
format Article
author Alwi, Aedrianee Reeza
Mahat, Naji Arafat
Mohd. Salleh, Faezah
Ishar, Seri Mirianti
Kamaluddin, Mohammad Rahim
A. Rashid, Mohd. Radzniwan
author_facet Alwi, Aedrianee Reeza
Mahat, Naji Arafat
Mohd. Salleh, Faezah
Ishar, Seri Mirianti
Kamaluddin, Mohammad Rahim
A. Rashid, Mohd. Radzniwan
author_sort Alwi, Aedrianee Reeza
title Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
title_short Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
title_full Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
title_fullStr Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
title_full_unstemmed Internal validation of reduced PCR reaction volume of the Qiagen Investigator® Argus X-12 QS Kit from blood samples on FTA cards.
title_sort internal validation of reduced pcr reaction volume of the qiagen investigator® argus x-12 qs kit from blood samples on fta cards.
publisher John Wiley and Sons Inc.
publishDate 2023
url http://eprints.utm.my/106682/
http://dx.doi.org/10.1111/1556-4029.15370
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score 13.211869

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