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Adhesive cryogel particles for bridging confined and irregular tissue defects

Background: Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging. Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies, making tissue bridging challenging. Methods: This study proposes a tissue adhesive in...

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Main Authors:	Xue, Yao Ting, Chen, Ming Yu, Cao, Jia Sheng, Wang, Lei, Hu, Jia Hao, Li, Si Yang, Shen, Ji Liang, Li, Xin Ge, Zhang, Kai Hang, Hao, Shu Qiang, Juengpanich, Sarun, Cheng, Si Bo, Wong, Tuck Whye, Yang, Xu Xu, Li, Tie Feng, Cai, Xiu Jun, Yang, Wei
Format:	Article
Language:	English
Published:	BioMed Central Ltd 2023
Subjects:	Q Science (General) TK Electrical engineering. Electronics Nuclear engineering TP Chemical technology
Online Access:	http://eprints.utm.my/105848/1/WongTuckWhye2023_AdhesiveCryogelParticlesforBridging.pdf http://eprints.utm.my/105848/ http://dx.doi.org/10.1186/s40779-023-00451-1
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spelling	my.utm.1058482024-05-20T07:10:19Z http://eprints.utm.my/105848/ Adhesive cryogel particles for bridging confined and irregular tissue defects Xue, Yao Ting Chen, Ming Yu Cao, Jia Sheng Wang, Lei Hu, Jia Hao Li, Si Yang Shen, Ji Liang Li, Xin Ge Zhang, Kai Hang Hao, Shu Qiang Juengpanich, Sarun Cheng, Si Bo Wong, Tuck Whye Yang, Xu Xu Li, Tie Feng Cai, Xiu Jun Yang, Wei Q Science (General) TK Electrical engineering. Electronics Nuclear engineering TP Chemical technology Background: Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging. Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies, making tissue bridging challenging. Methods: This study proposes a tissue adhesive in the form of adhesive cryogel particles (ACPs) made from chitosan, acrylic acid, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart, intestine, liver, muscle, and stomach. Cytotoxicity of ACPs was evaluated by cell proliferation of human normal liver cells (LO2) and human intestinal epithelial cells (Caco-2). The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat models. The ability of ACPs to bridge irregular tissue defects was assessed using porcine heart, liver, and kidney as the ex vivo models. Furthermore, a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness, biocompatibility, and applicability in clinical surgery. Results: ACPs are applicable to confined and irregular tissue defects, such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous organs. ACPs formed tough adhesion between tissues [(670.9 ± 50.1) J/m2 for the heart, (607.6 ± 30.0) J/m2 for the intestine, (473.7 ± 37.0) J/m2 for the liver, (186.1 ± 13.3) J/m2 for muscle, and (579.3 ± 32.3) J/m2 for the stomach]. ACPs showed considerable cytocompatibility in vitro study, with a high level of cell viability for 3 d [(98.8 ± 1.2) % for LO2 and (98.3 ± 1.6) % for Caco-2]. It has comparable inflammation repair in a ruptured rat liver (P = 0.58 compared with suture closure), the same with intestinal anastomosis in rabbits (P = 0.40 compared with suture anastomosis). Additionally, ACPs-based intestinal anastomosis (less than 30 s) was remarkably faster than the conventional suturing process (more than 10 min). When ACPs degrade after surgery, the tissues heal across the adhesion interface. Conclusions: ACPs are promising as the adhesive for clinical operations and battlefield rescue, with the capability to bridge irregular tissue defects rapidly. BioMed Central Ltd 2023-12 Article PeerReviewed application/pdf en http://eprints.utm.my/105848/1/WongTuckWhye2023_AdhesiveCryogelParticlesforBridging.pdf Xue, Yao Ting and Chen, Ming Yu and Cao, Jia Sheng and Wang, Lei and Hu, Jia Hao and Li, Si Yang and Shen, Ji Liang and Li, Xin Ge and Zhang, Kai Hang and Hao, Shu Qiang and Juengpanich, Sarun and Cheng, Si Bo and Wong, Tuck Whye and Yang, Xu Xu and Li, Tie Feng and Cai, Xiu Jun and Yang, Wei (2023) Adhesive cryogel particles for bridging confined and irregular tissue defects. Military Medical Research, 10 (1). pp. 1-15. ISSN 2095-7467 http://dx.doi.org/10.1186/s40779-023-00451-1 DOI:10.1186/s40779-023-00451-1
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	Q Science (General) TK Electrical engineering. Electronics Nuclear engineering TP Chemical technology
spellingShingle	Q Science (General) TK Electrical engineering. Electronics Nuclear engineering TP Chemical technology Xue, Yao Ting Chen, Ming Yu Cao, Jia Sheng Wang, Lei Hu, Jia Hao Li, Si Yang Shen, Ji Liang Li, Xin Ge Zhang, Kai Hang Hao, Shu Qiang Juengpanich, Sarun Cheng, Si Bo Wong, Tuck Whye Yang, Xu Xu Li, Tie Feng Cai, Xiu Jun Yang, Wei Adhesive cryogel particles for bridging confined and irregular tissue defects
description	Background: Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging. Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies, making tissue bridging challenging. Methods: This study proposes a tissue adhesive in the form of adhesive cryogel particles (ACPs) made from chitosan, acrylic acid, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart, intestine, liver, muscle, and stomach. Cytotoxicity of ACPs was evaluated by cell proliferation of human normal liver cells (LO2) and human intestinal epithelial cells (Caco-2). The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat models. The ability of ACPs to bridge irregular tissue defects was assessed using porcine heart, liver, and kidney as the ex vivo models. Furthermore, a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness, biocompatibility, and applicability in clinical surgery. Results: ACPs are applicable to confined and irregular tissue defects, such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous organs. ACPs formed tough adhesion between tissues [(670.9 ± 50.1) J/m2 for the heart, (607.6 ± 30.0) J/m2 for the intestine, (473.7 ± 37.0) J/m2 for the liver, (186.1 ± 13.3) J/m2 for muscle, and (579.3 ± 32.3) J/m2 for the stomach]. ACPs showed considerable cytocompatibility in vitro study, with a high level of cell viability for 3 d [(98.8 ± 1.2) % for LO2 and (98.3 ± 1.6) % for Caco-2]. It has comparable inflammation repair in a ruptured rat liver (P = 0.58 compared with suture closure), the same with intestinal anastomosis in rabbits (P = 0.40 compared with suture anastomosis). Additionally, ACPs-based intestinal anastomosis (less than 30 s) was remarkably faster than the conventional suturing process (more than 10 min). When ACPs degrade after surgery, the tissues heal across the adhesion interface. Conclusions: ACPs are promising as the adhesive for clinical operations and battlefield rescue, with the capability to bridge irregular tissue defects rapidly.
format	Article
author	Xue, Yao Ting Chen, Ming Yu Cao, Jia Sheng Wang, Lei Hu, Jia Hao Li, Si Yang Shen, Ji Liang Li, Xin Ge Zhang, Kai Hang Hao, Shu Qiang Juengpanich, Sarun Cheng, Si Bo Wong, Tuck Whye Yang, Xu Xu Li, Tie Feng Cai, Xiu Jun Yang, Wei
author_facet	Xue, Yao Ting Chen, Ming Yu Cao, Jia Sheng Wang, Lei Hu, Jia Hao Li, Si Yang Shen, Ji Liang Li, Xin Ge Zhang, Kai Hang Hao, Shu Qiang Juengpanich, Sarun Cheng, Si Bo Wong, Tuck Whye Yang, Xu Xu Li, Tie Feng Cai, Xiu Jun Yang, Wei
author_sort	Xue, Yao Ting
title	Adhesive cryogel particles for bridging confined and irregular tissue defects
title_short	Adhesive cryogel particles for bridging confined and irregular tissue defects
title_full	Adhesive cryogel particles for bridging confined and irregular tissue defects
title_fullStr	Adhesive cryogel particles for bridging confined and irregular tissue defects
title_full_unstemmed	Adhesive cryogel particles for bridging confined and irregular tissue defects
title_sort	adhesive cryogel particles for bridging confined and irregular tissue defects
publisher	BioMed Central Ltd
publishDate	2023
url	http://eprints.utm.my/105848/1/WongTuckWhye2023_AdhesiveCryogelParticlesforBridging.pdf http://eprints.utm.my/105848/ http://dx.doi.org/10.1186/s40779-023-00451-1
_version_	1800082672426418176
score	13.211869

Adhesive cryogel particles for bridging confined and irregular tissue defects

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