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Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface

Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI, however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based...

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Main Authors: Hu, Hui, Gopinath, Subash C. B., Ismail, Zool H., Chen, Yeng, Pandian, K., Velusamy, Palaniyandi
Format: Article
Published: John Wiley and Sons Inc 2023
Subjects:
T Technology (General)
Online Access:http://eprints.utm.my/105565/
http://dx.doi.org/10.1002/bab.2380
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spelling my.utm.1055652024-05-06T06:24:40Z http://eprints.utm.my/105565/ Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface Hu, Hui Gopinath, Subash C. B. Ismail, Zool H. Chen, Yeng Pandian, K. Velusamy, Palaniyandi T Technology (General) Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI, however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold-standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer-conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer-gold-cTnI-antibody sandwich pattern was 100 aM, while it was 1 fM with antibody-gold-cTnI-aptamer, representing 10-fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI. John Wiley and Sons Inc 2023 Article PeerReviewed Hu, Hui and Gopinath, Subash C. B. and Ismail, Zool H. and Chen, Yeng and Pandian, K. and Velusamy, Palaniyandi (2023) Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface. Biotechnology and Applied Biochemistry, 70 (2). pp. 581-591. ISSN 0885-4513 http://dx.doi.org/10.1002/bab.2380 DOI : 10.1002/bab.2380
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic T Technology (General)
spellingShingle T Technology (General)
Hu, Hui
Gopinath, Subash C. B.
Ismail, Zool H.
Chen, Yeng
Pandian, K.
Velusamy, Palaniyandi
Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
description Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI, however, these techniques are not sensitive enough for early-stage detection. A blood biomarker-based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold-standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer-conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer-gold-cTnI-antibody sandwich pattern was 100 aM, while it was 1 fM with antibody-gold-cTnI-aptamer, representing 10-fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI.
format Article
author Hu, Hui
Gopinath, Subash C. B.
Ismail, Zool H.
Chen, Yeng
Pandian, K.
Velusamy, Palaniyandi
author_facet Hu, Hui
Gopinath, Subash C. B.
Ismail, Zool H.
Chen, Yeng
Pandian, K.
Velusamy, Palaniyandi
author_sort Hu, Hui
title Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_short Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_full Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_fullStr Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_full_unstemmed Cardiovascular biomarker troponin I biosensor: Aptamer-gold-antibody hybrid on a metal oxide surface
title_sort cardiovascular biomarker troponin i biosensor: aptamer-gold-antibody hybrid on a metal oxide surface
publisher John Wiley and Sons Inc
publishDate 2023
url http://eprints.utm.my/105565/
http://dx.doi.org/10.1002/bab.2380
_version_ 1800082630168805376
score 13.211869

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