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Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products

Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification of herbal origin essential, especially for quality control. In recent decades, DNA-based methods have played a crucial...

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Main Authors: Izham, Nur Hazwani, Saidon, Nur Azreen, A. Samad, Abdul Fattah, Alina Wagiran, Alina Wagiran
Format: Article
Language:English
Published: Penerbit UTM Press 2023
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/105047/1/AlinaWagiran2023_EvaluationofDNAQualityfromModifiedDNA.pdf
http://eprints.utm.my/105047/
http://dx.doi.org/10.11113/jurnalteknologi.v85.18545
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spelling my.utm.1050472024-04-02T06:40:05Z http://eprints.utm.my/105047/ Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products Izham, Nur Hazwani Saidon, Nur Azreen A. Samad, Abdul Fattah Alina Wagiran, Alina Wagiran Q Science (General) Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification of herbal origin essential, especially for quality control. In recent decades, DNA-based methods have played a crucial role in the development of authentication tools that required good quality of DNA. The manufacturing process of herbal products involved heating, grinding or other mechanical procedures, and addition of excipients/additives caused DNA to degrade which in turn influenced DNA quality. In this study, nine different conventional methods with some modifications were evaluated to determine the best technique producing good quality DNA from capsule herbal products. Assessment was conducted using spectrophotometric measurements and agarose gel electrophoresis. To determine the quality of gDNA, amplification of ITS2 amplicon was performed by PCR. The DNA extraction finding showed that DNA quality from each method resulted in a different DNA purity and yield, hence the ITS2 amplification. Each of the modified DNA extraction methods performed has its own strengths and limitations when it comes to obtaining high quality gDNA. In addition, the study demonstrated the success of ITS2 amplification with the modified DNA extraction methods used. Penerbit UTM Press 2023-01 Article PeerReviewed application/pdf en http://eprints.utm.my/105047/1/AlinaWagiran2023_EvaluationofDNAQualityfromModifiedDNA.pdf Izham, Nur Hazwani and Saidon, Nur Azreen and A. Samad, Abdul Fattah and Alina Wagiran, Alina Wagiran (2023) Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products. Jurnal Teknologi, 85 (1). pp. 63-69. ISSN 0127-9696 http://dx.doi.org/10.11113/jurnalteknologi.v85.18545 DOI:10.11113/jurnalteknologi.v85.18545
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Izham, Nur Hazwani
Saidon, Nur Azreen
A. Samad, Abdul Fattah
Alina Wagiran, Alina Wagiran
Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
description Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification of herbal origin essential, especially for quality control. In recent decades, DNA-based methods have played a crucial role in the development of authentication tools that required good quality of DNA. The manufacturing process of herbal products involved heating, grinding or other mechanical procedures, and addition of excipients/additives caused DNA to degrade which in turn influenced DNA quality. In this study, nine different conventional methods with some modifications were evaluated to determine the best technique producing good quality DNA from capsule herbal products. Assessment was conducted using spectrophotometric measurements and agarose gel electrophoresis. To determine the quality of gDNA, amplification of ITS2 amplicon was performed by PCR. The DNA extraction finding showed that DNA quality from each method resulted in a different DNA purity and yield, hence the ITS2 amplification. Each of the modified DNA extraction methods performed has its own strengths and limitations when it comes to obtaining high quality gDNA. In addition, the study demonstrated the success of ITS2 amplification with the modified DNA extraction methods used.
format Article
author Izham, Nur Hazwani
Saidon, Nur Azreen
A. Samad, Abdul Fattah
Alina Wagiran, Alina Wagiran
author_facet Izham, Nur Hazwani
Saidon, Nur Azreen
A. Samad, Abdul Fattah
Alina Wagiran, Alina Wagiran
author_sort Izham, Nur Hazwani
title Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
title_short Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
title_full Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
title_fullStr Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
title_full_unstemmed Evaluation of DNA quality from modified DNA extraction and amplification of ITS2 from eurycoma longifolia capsule herbal products
title_sort evaluation of dna quality from modified dna extraction and amplification of its2 from eurycoma longifolia capsule herbal products
publisher Penerbit UTM Press
publishDate 2023
url http://eprints.utm.my/105047/1/AlinaWagiran2023_EvaluationofDNAQualityfromModifiedDNA.pdf
http://eprints.utm.my/105047/
http://dx.doi.org/10.11113/jurnalteknologi.v85.18545
_version_ 1797905926853754880
score 13.211869

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