Staff View: The effect of BPA-treated water on the small intestine via an in vivo study

The effect of BPA-treated water on the small intestine via an in vivo study

Since the major route of BPA exposure is via the oral route, BPA may have effects on the gastrointestinal tract, especially on the intestinal barrier, where most digestion and absorption processes occur. In this study, the effects of BPA‐treated water on the small intestine (SI) and SI tight junctio...

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Main Authors:	Kamaludin, Roziana, Rasdi, Zatilfarihiah, Othman, Mohd. Hafiz Dzarfan, Sheikh Abdul Kadir, Siti Hamimah, Idorus, Mohd. Yusri, Khan, Jesmine, Wan Mohamad Zain, Wan Nor I’zzah, Ismail, Ahmad Fauzi, A. Rahman, Mukhlis, Jaafar, Juhana
Format:	Article
Language:	English
Published:	MDPI 2022
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/104654/1/MohdHafizDzarfan2022_TheEffectofBPATreatedWaterontheSmall.pdf http://eprints.utm.my/104654/ http://dx.doi.org/10.3390/toxics10060296
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spelling	my.utm.1046542024-02-21T08:52:37Z http://eprints.utm.my/104654/ The effect of BPA-treated water on the small intestine via an in vivo study Kamaludin, Roziana Rasdi, Zatilfarihiah Othman, Mohd. Hafiz Dzarfan Sheikh Abdul Kadir, Siti Hamimah Idorus, Mohd. Yusri Khan, Jesmine Wan Mohamad Zain, Wan Nor I’zzah Ismail, Ahmad Fauzi A. Rahman, Mukhlis Jaafar, Juhana Q Science (General) Since the major route of BPA exposure is via the oral route, BPA may have effects on the gastrointestinal tract, especially on the intestinal barrier, where most digestion and absorption processes occur. In this study, the effects of BPA‐treated water on the small intestine (SI) and SI tight junction proteins (TJPs) of both pregnant Sprague–Dawley rats and their fetuses were inves-tigated. Previously, hybrid photocatalytic filtration treatment by a visible light driven N‐doped TiO2 membrane has successfully removed up to 81.6% of BPA in water. The effect of BPA‐untreated (5.00 ± ppm) and BPA‐treated water (0.9 ± ppm) after 21 days of exposure on the jejunum and ileum, as well as the expressions of claudin proteins, were investigated by Western blotting (WB) and hematoxylin and eosin (H&E) in order to investigate the potential of the photo-catalytic membrane in removing the detrimental effect of BPA. The results suggest that BPA exposure altered the morphology of villi, and affected the expression level of claudin‐2, ‐3, and ‐4 proteins in the jejunum and ileum of both pregnant rats and their fetuses. Interestingly, villi and claudins expressions were undisrupted in a treated‐BPA water group, which indicated that the degradation of BPA via membranes effectively mitigates the effect on BPA on gastrointestinal tract. MDPI 2022-06 Article PeerReviewed application/pdf en http://eprints.utm.my/104654/1/MohdHafizDzarfan2022_TheEffectofBPATreatedWaterontheSmall.pdf Kamaludin, Roziana and Rasdi, Zatilfarihiah and Othman, Mohd. Hafiz Dzarfan and Sheikh Abdul Kadir, Siti Hamimah and Idorus, Mohd. Yusri and Khan, Jesmine and Wan Mohamad Zain, Wan Nor I’zzah and Ismail, Ahmad Fauzi and A. Rahman, Mukhlis and Jaafar, Juhana (2022) The effect of BPA-treated water on the small intestine via an in vivo study. Toxics, 10 (6). pp. 1-15. ISSN 2305-6304 http://dx.doi.org/10.3390/toxics10060296 DOI:10.3390/toxics10060296
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kamaludin, Roziana Rasdi, Zatilfarihiah Othman, Mohd. Hafiz Dzarfan Sheikh Abdul Kadir, Siti Hamimah Idorus, Mohd. Yusri Khan, Jesmine Wan Mohamad Zain, Wan Nor I’zzah Ismail, Ahmad Fauzi A. Rahman, Mukhlis Jaafar, Juhana The effect of BPA-treated water on the small intestine via an in vivo study
description	Since the major route of BPA exposure is via the oral route, BPA may have effects on the gastrointestinal tract, especially on the intestinal barrier, where most digestion and absorption processes occur. In this study, the effects of BPA‐treated water on the small intestine (SI) and SI tight junction proteins (TJPs) of both pregnant Sprague–Dawley rats and their fetuses were inves-tigated. Previously, hybrid photocatalytic filtration treatment by a visible light driven N‐doped TiO2 membrane has successfully removed up to 81.6% of BPA in water. The effect of BPA‐untreated (5.00 ± ppm) and BPA‐treated water (0.9 ± ppm) after 21 days of exposure on the jejunum and ileum, as well as the expressions of claudin proteins, were investigated by Western blotting (WB) and hematoxylin and eosin (H&E) in order to investigate the potential of the photo-catalytic membrane in removing the detrimental effect of BPA. The results suggest that BPA exposure altered the morphology of villi, and affected the expression level of claudin‐2, ‐3, and ‐4 proteins in the jejunum and ileum of both pregnant rats and their fetuses. Interestingly, villi and claudins expressions were undisrupted in a treated‐BPA water group, which indicated that the degradation of BPA via membranes effectively mitigates the effect on BPA on gastrointestinal tract.
format	Article
author	Kamaludin, Roziana Rasdi, Zatilfarihiah Othman, Mohd. Hafiz Dzarfan Sheikh Abdul Kadir, Siti Hamimah Idorus, Mohd. Yusri Khan, Jesmine Wan Mohamad Zain, Wan Nor I’zzah Ismail, Ahmad Fauzi A. Rahman, Mukhlis Jaafar, Juhana
author_facet	Kamaludin, Roziana Rasdi, Zatilfarihiah Othman, Mohd. Hafiz Dzarfan Sheikh Abdul Kadir, Siti Hamimah Idorus, Mohd. Yusri Khan, Jesmine Wan Mohamad Zain, Wan Nor I’zzah Ismail, Ahmad Fauzi A. Rahman, Mukhlis Jaafar, Juhana
author_sort	Kamaludin, Roziana
title	The effect of BPA-treated water on the small intestine via an in vivo study
title_short	The effect of BPA-treated water on the small intestine via an in vivo study
title_full	The effect of BPA-treated water on the small intestine via an in vivo study
title_fullStr	The effect of BPA-treated water on the small intestine via an in vivo study
title_full_unstemmed	The effect of BPA-treated water on the small intestine via an in vivo study
title_sort	effect of bpa-treated water on the small intestine via an in vivo study
publisher	MDPI
publishDate	2022
url	http://eprints.utm.my/104654/1/MohdHafizDzarfan2022_TheEffectofBPATreatedWaterontheSmall.pdf http://eprints.utm.my/104654/ http://dx.doi.org/10.3390/toxics10060296
_version_	1792147920930209792
score	13.211869

The effect of BPA-treated water on the small intestine via an in vivo study

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