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Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation

Biogenic synthesis of silver nanoparticles (b-AgNPs) utilising plant extract has gained the interest of researchers due to the environmentally friendly and cost-effective technique. However, the extent of its application in the biomedical field remains scarce. This study evaluates the antibacterial,...

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Main Authors: Aminullah Lubis, Faridah, Nik Malek, Nik Ahmad Nizam, Sani, Nor Suriani, Jemon, Khairunadwa
Format: Article
Published: Elsevier B.V. 2022
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/103486/
http://dx.doi.org/10.1016/j.partic.2022.01.001
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spelling my.utm.1034862023-11-14T06:18:40Z http://eprints.utm.my/103486/ Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation Aminullah Lubis, Faridah Nik Malek, Nik Ahmad Nizam Sani, Nor Suriani Jemon, Khairunadwa Q Science (General) Biogenic synthesis of silver nanoparticles (b-AgNPs) utilising plant extract has gained the interest of researchers due to the environmentally friendly and cost-effective technique. However, the extent of its application in the biomedical field remains scarce. This study evaluates the antibacterial, cytocompatibility, and wound healing activities of synthesised AgNPs using Persicaria odorata leaves extract (PO-AgNPs). The formation of PO-AgNPs was observed by visual colour changes and verified by ultraviolet-visible (UV–vis) spectrophotometer, which revealed a surface plasmon resonance (SPR) at around 440 nm, and further confirmed by X-ray diffraction (XRD). Characterisation using Fourier transform infrared (FTIR) spectroscopy showed biomolecules from the leaves extract presented together on PO-AgNPs. Field emission scanning electron microscope (FESEM) and high-resolution transmission electron microscopy (HR-TEM) images revealed PO-AgNPs nanospheres with diameters of 11 ± 3 nm. Disc diffusion test (DDT) and minimum inhibitory concentration (MIC) analysis resulted in a dose-dependent inhibition of PO-AgNPs against tested Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus (MRSA). These results were further corroborated by time-kill kinetic assay which revealed that PO-AgNPs were bactericidal against both strains 24 h post-treatment. Cytocompatibility and in vitro wound healing evaluation against normal human fibroblast cells, HSF 1184 inferred that PO-AgNPs are non-toxic to normal cells and able to enhance cell migration as compared to the non-treated cells. Therefore, PO-AgNPs are biocompatible and possess antibacterial and wound healing capabilities that are useful in biomedical applications. Elsevier B.V. 2022-11 Article PeerReviewed Aminullah Lubis, Faridah and Nik Malek, Nik Ahmad Nizam and Sani, Nor Suriani and Jemon, Khairunadwa (2022) Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation. Particuology, 70 (NA). pp. 10-19. ISSN 1674-2001 http://dx.doi.org/10.1016/j.partic.2022.01.001 DOI:10.1016/j.partic.2022.01.001
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Aminullah Lubis, Faridah
Nik Malek, Nik Ahmad Nizam
Sani, Nor Suriani
Jemon, Khairunadwa
Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
description Biogenic synthesis of silver nanoparticles (b-AgNPs) utilising plant extract has gained the interest of researchers due to the environmentally friendly and cost-effective technique. However, the extent of its application in the biomedical field remains scarce. This study evaluates the antibacterial, cytocompatibility, and wound healing activities of synthesised AgNPs using Persicaria odorata leaves extract (PO-AgNPs). The formation of PO-AgNPs was observed by visual colour changes and verified by ultraviolet-visible (UV–vis) spectrophotometer, which revealed a surface plasmon resonance (SPR) at around 440 nm, and further confirmed by X-ray diffraction (XRD). Characterisation using Fourier transform infrared (FTIR) spectroscopy showed biomolecules from the leaves extract presented together on PO-AgNPs. Field emission scanning electron microscope (FESEM) and high-resolution transmission electron microscopy (HR-TEM) images revealed PO-AgNPs nanospheres with diameters of 11 ± 3 nm. Disc diffusion test (DDT) and minimum inhibitory concentration (MIC) analysis resulted in a dose-dependent inhibition of PO-AgNPs against tested Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus (MRSA). These results were further corroborated by time-kill kinetic assay which revealed that PO-AgNPs were bactericidal against both strains 24 h post-treatment. Cytocompatibility and in vitro wound healing evaluation against normal human fibroblast cells, HSF 1184 inferred that PO-AgNPs are non-toxic to normal cells and able to enhance cell migration as compared to the non-treated cells. Therefore, PO-AgNPs are biocompatible and possess antibacterial and wound healing capabilities that are useful in biomedical applications.
format Article
author Aminullah Lubis, Faridah
Nik Malek, Nik Ahmad Nizam
Sani, Nor Suriani
Jemon, Khairunadwa
author_facet Aminullah Lubis, Faridah
Nik Malek, Nik Ahmad Nizam
Sani, Nor Suriani
Jemon, Khairunadwa
author_sort Aminullah Lubis, Faridah
title Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
title_short Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
title_full Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
title_fullStr Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
title_full_unstemmed Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
title_sort biogenic synthesis of silver nanoparticles using persicaria odorata leaf extract: antibacterial, cytocompatibility, and in vitro wound healing evaluation
publisher Elsevier B.V.
publishDate 2022
url http://eprints.utm.my/103486/
http://dx.doi.org/10.1016/j.partic.2022.01.001
_version_ 1783876370114281472
score 13.211869

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