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Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples

Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determinat...

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Main Authors: Semail, Nadhiratul-Farihin, Abdul Keyon, Aemi Syazwani, Saad, Bahruddin, Kamaruzaman, Sazlinda, Mohamad Zain, Nur Nadhirah, Lim, Vuanghao, Miskam, Mazidatulakmam, Wan Abdullah, Wan Nazwanie, Raoov, Muggundha, Yahaya, Noorfatimah
Format: Article
Language:English
Published: Elsevier B.V. 2022
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/102954/1/AemiSyazwaniAbdul2022_InducedSampleViaTransientIsotachophoresis.pdf
http://eprints.utm.my/102954/
http://dx.doi.org/10.1016/j.chroma.2022.463616
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spelling my.utm.1029542023-10-12T08:23:43Z http://eprints.utm.my/102954/ Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples Semail, Nadhiratul-Farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Kamaruzaman, Sazlinda Mohamad Zain, Nur Nadhirah Lim, Vuanghao Miskam, Mazidatulakmam Wan Abdullah, Wan Nazwanie Raoov, Muggundha Yahaya, Noorfatimah Q Science (General) Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs); ketoprofen, diclofenac and naproxen from aqueous matrices. Prior to the system setup, various parameters were optimised to assess the potential use of the t-ITP paired with the sweeping stacking technique in micellar background electrolyte for dual preconcentration and separation of trace amounts of NSAIDs. Once the optimum conditions have been established, the method performance was validated and applied to 17 environmental water samples. Based on the results, the combined t-ITP and sweeping approach significantly improved the stacking and separation sensitivity. A large volume of samples could also be introduced and subsequently separated by MEKC with greater focusing effects due to the sweeping. Under optimised conditions, the developed method exhibited excellent linearity at a high range (0.1–500 ng/mL, r2 ≥ 0.998), low limits of detection (LODs) of 0.01–0.07 ng/mL, and a remarkable relative recovery (RR) of 99.6–101.9% with a relative standard deviation (RSD) of 1.4–8.6% (n = 9). Ultimately, the sensitivity enhancement factors improved up to 666-fold using the optimised method. Therefore, the proposed method presents a simplified yet effective and suitable for the determination of NSAIDs from aqueous matrices. Elsevier B.V. 2022-12 Article PeerReviewed application/pdf en http://eprints.utm.my/102954/1/AemiSyazwaniAbdul2022_InducedSampleViaTransientIsotachophoresis.pdf Semail, Nadhiratul-Farihin and Abdul Keyon, Aemi Syazwani and Saad, Bahruddin and Kamaruzaman, Sazlinda and Mohamad Zain, Nur Nadhirah and Lim, Vuanghao and Miskam, Mazidatulakmam and Wan Abdullah, Wan Nazwanie and Raoov, Muggundha and Yahaya, Noorfatimah (2022) Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples. Journal of Chromatography A, 1685 (463616). pp. 1-10. ISSN 0021-9673 http://dx.doi.org/10.1016/j.chroma.2022.463616 DOI: 10.1016/j.chroma.2022.463616
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Semail, Nadhiratul-Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
description Realising the need to devise a simple, sensitive, and reliable detection method, this study investigated the development of a dual-stacking transient isotachophoresis (t-ITP) and sweeping in micellar electrokinetic chromatography with diode array detector (t-ITP/sweeping-MEKC-DAD) for the determination of selected non-steroidal anti-inflammatory drugs (NSAIDs); ketoprofen, diclofenac and naproxen from aqueous matrices. Prior to the system setup, various parameters were optimised to assess the potential use of the t-ITP paired with the sweeping stacking technique in micellar background electrolyte for dual preconcentration and separation of trace amounts of NSAIDs. Once the optimum conditions have been established, the method performance was validated and applied to 17 environmental water samples. Based on the results, the combined t-ITP and sweeping approach significantly improved the stacking and separation sensitivity. A large volume of samples could also be introduced and subsequently separated by MEKC with greater focusing effects due to the sweeping. Under optimised conditions, the developed method exhibited excellent linearity at a high range (0.1–500 ng/mL, r2 ≥ 0.998), low limits of detection (LODs) of 0.01–0.07 ng/mL, and a remarkable relative recovery (RR) of 99.6–101.9% with a relative standard deviation (RSD) of 1.4–8.6% (n = 9). Ultimately, the sensitivity enhancement factors improved up to 666-fold using the optimised method. Therefore, the proposed method presents a simplified yet effective and suitable for the determination of NSAIDs from aqueous matrices.
format Article
author Semail, Nadhiratul-Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
author_facet Semail, Nadhiratul-Farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Kamaruzaman, Sazlinda
Mohamad Zain, Nur Nadhirah
Lim, Vuanghao
Miskam, Mazidatulakmam
Wan Abdullah, Wan Nazwanie
Raoov, Muggundha
Yahaya, Noorfatimah
author_sort Semail, Nadhiratul-Farihin
title Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_short Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_full Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_fullStr Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_full_unstemmed Induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
title_sort induced sample via transient isotachophoresis mediated with sweeping in micellar electrokinetic chromatography for the dual-stacking strategy of non-steroidal anti-inflammatory drugs in environmental water samples
publisher Elsevier B.V.
publishDate 2022
url http://eprints.utm.my/102954/1/AemiSyazwaniAbdul2022_InducedSampleViaTransientIsotachophoresis.pdf
http://eprints.utm.my/102954/
http://dx.doi.org/10.1016/j.chroma.2022.463616
_version_ 1781777622678634496
score 13.211869

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