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Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment

Tumor microenvironment has been identified as a crucial player in influencing anti-tumor immune response in breast cancer. Alternative treatment using combination of carbon nanotubes (CNTs) with hyperthermia (HT) has been reported to reduce tumor burden. However, its immunological mechanisms in tumo...

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Main Author: Johari, Nur Amanina
Format: Thesis
Language:English
Published: 2020
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/102265/1/NurAmaninaJohariMFS2020.pdf.pdf
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spelling my.utm.1022652023-08-14T06:26:42Z http://eprints.utm.my/id/eprint/102265/ Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment Johari, Nur Amanina QD Chemistry Tumor microenvironment has been identified as a crucial player in influencing anti-tumor immune response in breast cancer. Alternative treatment using combination of carbon nanotubes (CNTs) with hyperthermia (HT) has been reported to reduce tumor burden. However, its immunological mechanisms in tumor microenvironment is not yet fully understood. Hence, this study was conducted to investigate the effect of combination of ox-MWCNT and hyperthermia treatment on immune responses in tumor microenvironment. In this study, EMT6 breast cancer cells were inoculated subcutaneously into right flank of female Balb/c mice. At day 7 post-inoculation, ox-MWCNT were injected intratumorally in CNT and combined groups. Then, mice in combined and HT were subjected to local HT for three consecutive days. Mice for control group was left untreated. Mice were euthanized 10 days post-inoculation and their lymph nodes were harvested for flow cytometric (FACS) analysis while the tumors were subjected for immunohistochemistry (IHC) analysis. In another experiment, mice were euthanized 21 days post-inoculation and tumors were harvested for FACS analysis. Results from this study demonstrated that the infiltration and maturation of DCs in lymph nodes increased in combined treated groups. In addition, FACS analysis of tumor cells showed that combined treatment significantly increased the infiltration of CD8+ and CD4+ T cells, natural killer (NK) cells and macrophages. Furthermore, combined and HT alone has shown to drastically decrease Tregs population in tumor. On the other hand, data from the IHC studies indicated that angiogenesis activity increased in combination therapy but had less influence on inducing tumor progression. Data presented in this study highlights the potential therapeutic use of ox-MWCNT with HT treatment against breast cancer. Information gained from this research may contribute to understand on the effect of combined treatment on tumor microenvironment and may have implications in breast cancer management in clinical setting. 2020 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/102265/1/NurAmaninaJohariMFS2020.pdf.pdf Johari, Nur Amanina (2020) Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment. Masters thesis, Universiti Teknologi Malaysia. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146351
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Johari, Nur Amanina
Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
description Tumor microenvironment has been identified as a crucial player in influencing anti-tumor immune response in breast cancer. Alternative treatment using combination of carbon nanotubes (CNTs) with hyperthermia (HT) has been reported to reduce tumor burden. However, its immunological mechanisms in tumor microenvironment is not yet fully understood. Hence, this study was conducted to investigate the effect of combination of ox-MWCNT and hyperthermia treatment on immune responses in tumor microenvironment. In this study, EMT6 breast cancer cells were inoculated subcutaneously into right flank of female Balb/c mice. At day 7 post-inoculation, ox-MWCNT were injected intratumorally in CNT and combined groups. Then, mice in combined and HT were subjected to local HT for three consecutive days. Mice for control group was left untreated. Mice were euthanized 10 days post-inoculation and their lymph nodes were harvested for flow cytometric (FACS) analysis while the tumors were subjected for immunohistochemistry (IHC) analysis. In another experiment, mice were euthanized 21 days post-inoculation and tumors were harvested for FACS analysis. Results from this study demonstrated that the infiltration and maturation of DCs in lymph nodes increased in combined treated groups. In addition, FACS analysis of tumor cells showed that combined treatment significantly increased the infiltration of CD8+ and CD4+ T cells, natural killer (NK) cells and macrophages. Furthermore, combined and HT alone has shown to drastically decrease Tregs population in tumor. On the other hand, data from the IHC studies indicated that angiogenesis activity increased in combination therapy but had less influence on inducing tumor progression. Data presented in this study highlights the potential therapeutic use of ox-MWCNT with HT treatment against breast cancer. Information gained from this research may contribute to understand on the effect of combined treatment on tumor microenvironment and may have implications in breast cancer management in clinical setting.
format Thesis
author Johari, Nur Amanina
author_facet Johari, Nur Amanina
author_sort Johari, Nur Amanina
title Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
title_short Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
title_full Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
title_fullStr Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
title_full_unstemmed Effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on EMT6 tumor microenvironment
title_sort effect of oxidized multiwalled carbon nanotubes and hyperthermia treatment on emt6 tumor microenvironment
publishDate 2020
url http://eprints.utm.my/id/eprint/102265/1/NurAmaninaJohariMFS2020.pdf.pdf
http://eprints.utm.my/id/eprint/102265/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146351
_version_ 1775621962197893120
score 13.211869

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