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Bioactivity study of protease digested peptides from Moringa Oleifera seeds

Moringa oleifera is an important plant for centuries due to its nutritional properties, medicinal importance, and lately, wastewater treatment. Almost all parts of this plant contribute to its nutritional properties. Many studies have reported the importance of the bioactivity of the proteins from M...

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Main Author: Danishfar, Farid Ahmad
Format: Thesis
Language:English
Published: 2020
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/101870/1/FaridAhmadDanishfarMFS2020.pdf
http://eprints.utm.my/id/eprint/101870/
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spelling my.utm.1018702023-07-17T02:37:26Z http://eprints.utm.my/id/eprint/101870/ Bioactivity study of protease digested peptides from Moringa Oleifera seeds Danishfar, Farid Ahmad QD Chemistry Moringa oleifera is an important plant for centuries due to its nutritional properties, medicinal importance, and lately, wastewater treatment. Almost all parts of this plant contribute to its nutritional properties. Many studies have reported the importance of the bioactivity of the proteins from M. oleifera seed like antioxidant, antidiabetic, antihypertensive and wastewater treatment, but there is no investigation on the bioactivity of digested products of M. oleifera fresh seed proteins that could be used for therapeutic activity. The objectives of this research were to determine the antioxidant and a-amylase inhibition activities of Moringa oleifera fresh seed proteins and their digested products. The seed protein was extracted using Tris-HCl buffer followed by quantification by Bradford Assay and quality checked by Sodium Dodecyl-Sulphate Polyacrylamide gel electrophoresis (SDS-PAGE). Then, proteins were digested using proteolytic enzymes (pepsin, trypsin, and chymotrypsin). Next, the antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power (FRAP) assay. After that, the alpha-amylase inhibition was determined by a-amylase inhibition assay. All digested products showed higher antioxidant activity than undigested protein. Pepsin digestion products showed the highest Radical scavenging activity about 30.86±2.86 %RSA than other digestion products. But in terms of FRAP assay, trypsin digestion products showed better ability about 5.51±0.009 Fe2+/g than pepsin and chymotrypsin digestion. Based on the a-amylase inhibition assay, all digested products showed good inhibitory activity, but, interestingly, the undigested protein had higher inhibition activity than digestion products. In conclusion, this study showed that digestion with proteases has improved the antioxidant activity of M. oleifera fresh seed proteins. In addition, the digestion products have inhibition of a-amylase activity but digestion did not increase the inhibitory activity. The results provide information on antioxidant and enzyme inhibitory as well as comparison of M. oleifera seed protein and the effect of protein digested by different proteases that could contribute to pharmaceutical and functional food. 2020 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/101870/1/FaridAhmadDanishfarMFS2020.pdf Danishfar, Farid Ahmad (2020) Bioactivity study of protease digested peptides from Moringa Oleifera seeds. Masters thesis, Universiti Teknologi Malaysia. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146374
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Danishfar, Farid Ahmad
Bioactivity study of protease digested peptides from Moringa Oleifera seeds
description Moringa oleifera is an important plant for centuries due to its nutritional properties, medicinal importance, and lately, wastewater treatment. Almost all parts of this plant contribute to its nutritional properties. Many studies have reported the importance of the bioactivity of the proteins from M. oleifera seed like antioxidant, antidiabetic, antihypertensive and wastewater treatment, but there is no investigation on the bioactivity of digested products of M. oleifera fresh seed proteins that could be used for therapeutic activity. The objectives of this research were to determine the antioxidant and a-amylase inhibition activities of Moringa oleifera fresh seed proteins and their digested products. The seed protein was extracted using Tris-HCl buffer followed by quantification by Bradford Assay and quality checked by Sodium Dodecyl-Sulphate Polyacrylamide gel electrophoresis (SDS-PAGE). Then, proteins were digested using proteolytic enzymes (pepsin, trypsin, and chymotrypsin). Next, the antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power (FRAP) assay. After that, the alpha-amylase inhibition was determined by a-amylase inhibition assay. All digested products showed higher antioxidant activity than undigested protein. Pepsin digestion products showed the highest Radical scavenging activity about 30.86±2.86 %RSA than other digestion products. But in terms of FRAP assay, trypsin digestion products showed better ability about 5.51±0.009 Fe2+/g than pepsin and chymotrypsin digestion. Based on the a-amylase inhibition assay, all digested products showed good inhibitory activity, but, interestingly, the undigested protein had higher inhibition activity than digestion products. In conclusion, this study showed that digestion with proteases has improved the antioxidant activity of M. oleifera fresh seed proteins. In addition, the digestion products have inhibition of a-amylase activity but digestion did not increase the inhibitory activity. The results provide information on antioxidant and enzyme inhibitory as well as comparison of M. oleifera seed protein and the effect of protein digested by different proteases that could contribute to pharmaceutical and functional food.
format Thesis
author Danishfar, Farid Ahmad
author_facet Danishfar, Farid Ahmad
author_sort Danishfar, Farid Ahmad
title Bioactivity study of protease digested peptides from Moringa Oleifera seeds
title_short Bioactivity study of protease digested peptides from Moringa Oleifera seeds
title_full Bioactivity study of protease digested peptides from Moringa Oleifera seeds
title_fullStr Bioactivity study of protease digested peptides from Moringa Oleifera seeds
title_full_unstemmed Bioactivity study of protease digested peptides from Moringa Oleifera seeds
title_sort bioactivity study of protease digested peptides from moringa oleifera seeds
publishDate 2020
url http://eprints.utm.my/id/eprint/101870/1/FaridAhmadDanishfarMFS2020.pdf
http://eprints.utm.my/id/eprint/101870/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146374
_version_ 1772811124327055360
score 13.211869

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