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Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties

Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combi...

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Main Authors: Jailani, Nashriq, Jaafar, Nardiah Rizwana, Suhaimi, Suhaily, Mackeen, Mukram Mohamed, Abu Bakar, Farah Diba
Format: Article
Language:English
Published: Elsevier B.V. 2022
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf
http://eprints.utm.my/id/eprint/100998/
http://dx.doi.org/10.1016/j.ijbiomac.2022.05.170
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spelling my.utm.1009982023-05-18T06:13:38Z http://eprints.utm.my/id/eprint/100998/ Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties Jailani, Nashriq Jaafar, Nardiah Rizwana Suhaimi, Suhaily Mackeen, Mukram Mohamed Abu Bakar, Farah Diba TP Chemical technology Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combined the molecular modeling and experimental optimization to investigate the influences of different cross-linking agents in developing CLEA of cyclodextrin glucanotranferase G1 (CGTase G1) for cyclodextrins (CDs) synthesis. Seven types of cross-linkers were tested and CGTase G1 cross-linked with chitosan (CS-CGTG1-CLEA) displayed the highest activity recovery (84.6 ± 0.26%), aligning with its highest binding affinity, radius of gyration and flexibility through in-silico analysis towards CGTase G1. CS-CGTG1-CLEA was characterized and showed a longer half-life (30.06 ± 1.51 min) and retained a greater thermal stability (52.73 ± 0.93%) after 30 min incubation at optimal conditions compared to free enzyme (10.30 ± 1.34 min and 5.51 ± 2.10% respectively). CS-CGTG1-CLEA improved CDs production by 33% and yielded cumulative of 52.62 g/L CDs after five cycles for 2 h of reaction. This study reveals that abundant of hydroxyl group on chitosan interacted with CGTase G1 surface amino acid residues to form strong and stable CLEA thus can be a promising biocatalyst in CDs production. Elsevier B.V. 2022 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf Jailani, Nashriq and Jaafar, Nardiah Rizwana and Suhaimi, Suhaily and Mackeen, Mukram Mohamed and Abu Bakar, Farah Diba (2022) Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties. International Journal of Biological Macromolecules, 213 (-). pp. 516-533. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2022.05.170 DOI: 10.1016/j.ijbiomac.2022.05.170
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Jailani, Nashriq
Jaafar, Nardiah Rizwana
Suhaimi, Suhaily
Mackeen, Mukram Mohamed
Abu Bakar, Farah Diba
Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
description Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combined the molecular modeling and experimental optimization to investigate the influences of different cross-linking agents in developing CLEA of cyclodextrin glucanotranferase G1 (CGTase G1) for cyclodextrins (CDs) synthesis. Seven types of cross-linkers were tested and CGTase G1 cross-linked with chitosan (CS-CGTG1-CLEA) displayed the highest activity recovery (84.6 ± 0.26%), aligning with its highest binding affinity, radius of gyration and flexibility through in-silico analysis towards CGTase G1. CS-CGTG1-CLEA was characterized and showed a longer half-life (30.06 ± 1.51 min) and retained a greater thermal stability (52.73 ± 0.93%) after 30 min incubation at optimal conditions compared to free enzyme (10.30 ± 1.34 min and 5.51 ± 2.10% respectively). CS-CGTG1-CLEA improved CDs production by 33% and yielded cumulative of 52.62 g/L CDs after five cycles for 2 h of reaction. This study reveals that abundant of hydroxyl group on chitosan interacted with CGTase G1 surface amino acid residues to form strong and stable CLEA thus can be a promising biocatalyst in CDs production.
format Article
author Jailani, Nashriq
Jaafar, Nardiah Rizwana
Suhaimi, Suhaily
Mackeen, Mukram Mohamed
Abu Bakar, Farah Diba
author_facet Jailani, Nashriq
Jaafar, Nardiah Rizwana
Suhaimi, Suhaily
Mackeen, Mukram Mohamed
Abu Bakar, Farah Diba
author_sort Jailani, Nashriq
title Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
title_short Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
title_full Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
title_fullStr Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
title_full_unstemmed Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
title_sort cross-linked cyclodextrin glucanotransferase aggregates from bacillus lehensis g1 for cyclodextrin production: molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
publisher Elsevier B.V.
publishDate 2022
url http://eprints.utm.my/id/eprint/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf
http://eprints.utm.my/id/eprint/100998/
http://dx.doi.org/10.1016/j.ijbiomac.2022.05.170
_version_ 1768006594937749504
score 13.211869

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