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Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis

Kelulut honey (KH) has a broad spectrum of antibacterial effects that conceivably deteriorate upon heat treatment. However, further synergistic combinations with plant extract might be of value in improving the bactericidal capacity. Therefore, this study reports on the combination of KH with Phylla...

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Main Authors: Razali, Muhammad Faiz, Jenorico Aripin, Jenorico Aripin, Iderus, Nadia Natasha, Mohd Adnan, Nuraisha Syazwani, Mohd Fauzi, Noor Akhmazillah
Format: Article
Language:English
Published: 2023
Subjects:
T Technology (General)
Online Access:http://eprints.uthm.edu.my/9641/1/J16165_6ba2ddc354d5846801b7ca0f3110687c.pdf
http://eprints.uthm.edu.my/9641/
https://doi.org/10.48048/tis.2023.5942
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spelling my.uthm.eprints.96412023-08-16T07:10:26Z http://eprints.uthm.edu.my/9641/ Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis Razali, Muhammad Faiz Jenorico Aripin, Jenorico Aripin Iderus, Nadia Natasha Mohd Adnan, Nuraisha Syazwani Mohd Fauzi, Noor Akhmazillah T Technology (General) Kelulut honey (KH) has a broad spectrum of antibacterial effects that conceivably deteriorate upon heat treatment. However, further synergistic combinations with plant extract might be of value in improving the bactericidal capacity. Therefore, this study reports on the combination of KH with Phyllanthus niruri extract as both are independently known to be bactericidal. P.niruri extract was incorporated into 2 types of KH; untreated and thermal-treated (50, 60 and 70 °C for 5, 10, 15 and 30 min); and tested against 4 bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis) through disc diffusion method. Untreated KH in general had a bigger inhibition zone than thermal-treated. The inhibition reaction was considered sensitive (> 8 mm) against S.aureus, E.coli and P.aeruginosa. Whereas, the effect of thermal treatment on KH was significantly (p < 0.05) apparent against S.aureus, in which the inhibition zones were mostly not sensitive (< 8 mm). In combination, the mixture of 70:30 (KH:P.niruri) had the highest inhibition zone as compared to other ratios. However, the interaction was only deemed to be synergistic with untreated KH, principally against S.aures and P.aeruginosa. The results thus showed the vast inhibition spectrum of KH as affected by thermal treatment and combination with P.niruri extract. Thermal treatment reduced the bactericidal of KH while incorporation with P.niruri extract could synergistically improve the bactericidal with a ratio of 70:30. 2023 Article PeerReviewed text en http://eprints.uthm.edu.my/9641/1/J16165_6ba2ddc354d5846801b7ca0f3110687c.pdf Razali, Muhammad Faiz and Jenorico Aripin, Jenorico Aripin and Iderus, Nadia Natasha and Mohd Adnan, Nuraisha Syazwani and Mohd Fauzi, Noor Akhmazillah (2023) Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. TRENDS IN SCIENCES, 20 (10). pp. 1-13. https://doi.org/10.48048/tis.2023.5942
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Razali, Muhammad Faiz
Jenorico Aripin, Jenorico Aripin
Iderus, Nadia Natasha
Mohd Adnan, Nuraisha Syazwani
Mohd Fauzi, Noor Akhmazillah
Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
description Kelulut honey (KH) has a broad spectrum of antibacterial effects that conceivably deteriorate upon heat treatment. However, further synergistic combinations with plant extract might be of value in improving the bactericidal capacity. Therefore, this study reports on the combination of KH with Phyllanthus niruri extract as both are independently known to be bactericidal. P.niruri extract was incorporated into 2 types of KH; untreated and thermal-treated (50, 60 and 70 °C for 5, 10, 15 and 30 min); and tested against 4 bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis) through disc diffusion method. Untreated KH in general had a bigger inhibition zone than thermal-treated. The inhibition reaction was considered sensitive (> 8 mm) against S.aureus, E.coli and P.aeruginosa. Whereas, the effect of thermal treatment on KH was significantly (p < 0.05) apparent against S.aureus, in which the inhibition zones were mostly not sensitive (< 8 mm). In combination, the mixture of 70:30 (KH:P.niruri) had the highest inhibition zone as compared to other ratios. However, the interaction was only deemed to be synergistic with untreated KH, principally against S.aures and P.aeruginosa. The results thus showed the vast inhibition spectrum of KH as affected by thermal treatment and combination with P.niruri extract. Thermal treatment reduced the bactericidal of KH while incorporation with P.niruri extract could synergistically improve the bactericidal with a ratio of 70:30.
format Article
author Razali, Muhammad Faiz
Jenorico Aripin, Jenorico Aripin
Iderus, Nadia Natasha
Mohd Adnan, Nuraisha Syazwani
Mohd Fauzi, Noor Akhmazillah
author_facet Razali, Muhammad Faiz
Jenorico Aripin, Jenorico Aripin
Iderus, Nadia Natasha
Mohd Adnan, Nuraisha Syazwani
Mohd Fauzi, Noor Akhmazillah
author_sort Razali, Muhammad Faiz
title Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
title_short Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
title_full Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
title_fullStr Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
title_full_unstemmed Combination of Thermal-Treated Kelulut (Stingless Bee) Honey and Phyllanthus niruri Extract: Evaluation on Antibacterial Activity Against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis
title_sort combination of thermal-treated kelulut (stingless bee) honey and phyllanthus niruri extract: evaluation on antibacterial activity against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and staphylococcus epidermidis
publishDate 2023
url http://eprints.uthm.edu.my/9641/1/J16165_6ba2ddc354d5846801b7ca0f3110687c.pdf
http://eprints.uthm.edu.my/9641/
https://doi.org/10.48048/tis.2023.5942
_version_ 1775624949975744512
score 13.211869

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