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Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications

A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Alginate (Alg) and chitosan (Chi) are both popular materials applied as biomaterials or bioimplants. However, Alg derived from brown algae is highly compliant and easily deco...

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Main Author: Ramli, Roslinda Hani
Format: Thesis
Language:English
English
English
Published: 2017
Subjects:
R Medicine (General)
Online Access:http://eprints.uthm.edu.my/856/1/24p%20ROSLINDA%20HANI%20RAMLI.pdf
http://eprints.uthm.edu.my/856/2/ROSLINDA%20HANI%20RAMLI%20COPYRIGHT%20DECLARATION.pdf
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http://eprints.uthm.edu.my/856/
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spelling my.uthm.eprints.8562021-09-06T05:40:56Z http://eprints.uthm.edu.my/856/ Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications Ramli, Roslinda Hani R Medicine (General) A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Alginate (Alg) and chitosan (Chi) are both popular materials applied as biomaterials or bioimplants. However, Alg derived from brown algae is highly compliant and easily decomposed in fluid, whilst Chi derived from shrimp shells has weak strength. In rectify these problems, the development of Chi and Alg based biodegradable scaffolds incorporated with silver nanoparticles (AgNPs) with enhanced mechanical properties and biosafe function is proposed. Different ratios of chitosan/alginate (Chi/Alg) were prepared and the effect of different ratio (1:1, 1:2 and 2:1) to the mechanical, biological properties with and without AgNPs and keratinocyte cell growth were investigated. The preliminary result of FTIR, UV-Vis, XRD, FESEM and EDS proved the production of silver nanoparticles. Meanwhile, FTIR, swelling/degradation, DMA, TGA, DSC, FESEM and MTT assay was conducted to study the properties of Chi/Alg based scaffold. FTIR analysis shows the crosslinking of Chi/Alg based scaffold. Swelling/degradation and DMA shows Chi/Alg and chitosan/alginate/silver nanoparticles (Chi/Alg/AgNPs) has adequate swelling and compressive modulus that exceed the epidermis’ Young modulus, thus able to provide mechanical support upon application. Meanwhile, the thermal analysis revealed that the onset decomposition temperature of scaffold were at around 70 ºC which is due to the loss of water present in the scaffold thus thermally safe for soft tissue application. Based on FESEM result, there are different in surface structure of Chi/Alg based scaffold. Finally, with the incorporation of 0.3 % PVP synthesised AgNPs in Chi/Alg based scaffold, cells are able to live up to 14 days. As a result, Chi incorporation in the Alg and AgNPs improved physical, mechanical properties of hydrogel itself and provide biosafe environment during the study. 2017-08 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/856/1/24p%20ROSLINDA%20HANI%20RAMLI.pdf text en http://eprints.uthm.edu.my/856/2/ROSLINDA%20HANI%20RAMLI%20COPYRIGHT%20DECLARATION.pdf text en http://eprints.uthm.edu.my/856/3/ROSLINDA%20HANI%20RAMLI%20WATERMARK.pdf Ramli, Roslinda Hani (2017) Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications. Masters thesis, Universiti Tun Hussein Onn Malaysia.
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
English
English
topic R Medicine (General)
spellingShingle R Medicine (General)
Ramli, Roslinda Hani
Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
description A biodegradable scaffold in tissue engineering serves as a temporary skeleton to accommodate and stimulate new tissue growth. Alginate (Alg) and chitosan (Chi) are both popular materials applied as biomaterials or bioimplants. However, Alg derived from brown algae is highly compliant and easily decomposed in fluid, whilst Chi derived from shrimp shells has weak strength. In rectify these problems, the development of Chi and Alg based biodegradable scaffolds incorporated with silver nanoparticles (AgNPs) with enhanced mechanical properties and biosafe function is proposed. Different ratios of chitosan/alginate (Chi/Alg) were prepared and the effect of different ratio (1:1, 1:2 and 2:1) to the mechanical, biological properties with and without AgNPs and keratinocyte cell growth were investigated. The preliminary result of FTIR, UV-Vis, XRD, FESEM and EDS proved the production of silver nanoparticles. Meanwhile, FTIR, swelling/degradation, DMA, TGA, DSC, FESEM and MTT assay was conducted to study the properties of Chi/Alg based scaffold. FTIR analysis shows the crosslinking of Chi/Alg based scaffold. Swelling/degradation and DMA shows Chi/Alg and chitosan/alginate/silver nanoparticles (Chi/Alg/AgNPs) has adequate swelling and compressive modulus that exceed the epidermis’ Young modulus, thus able to provide mechanical support upon application. Meanwhile, the thermal analysis revealed that the onset decomposition temperature of scaffold were at around 70 ºC which is due to the loss of water present in the scaffold thus thermally safe for soft tissue application. Based on FESEM result, there are different in surface structure of Chi/Alg based scaffold. Finally, with the incorporation of 0.3 % PVP synthesised AgNPs in Chi/Alg based scaffold, cells are able to live up to 14 days. As a result, Chi incorporation in the Alg and AgNPs improved physical, mechanical properties of hydrogel itself and provide biosafe environment during the study.
format Thesis
author Ramli, Roslinda Hani
author_facet Ramli, Roslinda Hani
author_sort Ramli, Roslinda Hani
title Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
title_short Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
title_full Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
title_fullStr Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
title_full_unstemmed Development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
title_sort development of chitosan/alginate/silver nanoparticles hydrogel scaffold for soft tissue engineering applications
publishDate 2017
url http://eprints.uthm.edu.my/856/1/24p%20ROSLINDA%20HANI%20RAMLI.pdf
http://eprints.uthm.edu.my/856/2/ROSLINDA%20HANI%20RAMLI%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/856/3/ROSLINDA%20HANI%20RAMLI%20WATERMARK.pdf
http://eprints.uthm.edu.my/856/
_version_ 1738580791116431360
score 13.211869

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