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Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production

Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase...

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Main Author: Abdul Manaf, Shoriya Aruni
Format: Thesis
Language:English
English
English
Published: 2022
Subjects:
TP Chemical technology
Online Access:http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf
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spelling my.uthm.eprints.84862023-04-02T00:53:18Z http://eprints.uthm.edu.my/8486/ Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production Abdul Manaf, Shoriya Aruni TP Chemical technology Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase production that is simple and less time-consuming is desired. The goal of this research is to produce a large-scale xylanase by immobilizing recombinant Kluyveromyces lactis with carbon nanomaterial and to apply a direct whole cell biocatalyst method for xylooligosaccharides production. Therefore, four carbon nanomaterials were screened using the pretreatment process that measured xylanase activity and cell growth. Carbon nanotubes (CNT) and graphene oxide (GO) were analyzed and their immobilization and culture condition factors were optimized using Response Surface Methodology (RSM) with different design models, as well as large-scale production process using a bioreactor. Analysis on the carbon nanomaterial was done using a Field Emission Scanning Electron Microscopy with Energy Dispersive X-ray (FESEM-EDX) and Fourier Transform Infrared spectroscopy (FTIR) while Ultra High-Performance Liquid Chromatography (UHPLC) was used to analyze the final sugar product. The most important factors in xylanase production with low cell leakage are cell loading and agar concentration. Following RSM screening and optimization, the xylanase production from free cells (1.39 U/mL) increased tenfold after cell immobilization (10.30 U/mL), and increased to 15 U/mL during the upscale process in the bioreactor. The immobilized cells can be reused for up to 7 fermentation cycles and stored at 4 ℃ for up to 90 days. The end products of lignocellulosic biomass bioconversion are xylobiose and xylotriose. Cell immobilization with carbon nanomaterials has been shown to successfully enhance xylanase production, opening up a new path to improved bioprocessing, particularly for the production of enzymes with reusability and long-term storage. 2022-09 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf text en http://eprints.uthm.edu.my/8486/2/SHORIYA%20ARUNI%20ABDUL%20MANAF%20COPYRIGHT%20DECLARATION.pdf text en http://eprints.uthm.edu.my/8486/3/SHORIYA%20ARUNI%20ABDUL%20MANAF%20WATERMARK.pdf Abdul Manaf, Shoriya Aruni (2022) Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production. Doctoral thesis, Universiti Tun Hussein Onn Malaysia.
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abdul Manaf, Shoriya Aruni
Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
description Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase production that is simple and less time-consuming is desired. The goal of this research is to produce a large-scale xylanase by immobilizing recombinant Kluyveromyces lactis with carbon nanomaterial and to apply a direct whole cell biocatalyst method for xylooligosaccharides production. Therefore, four carbon nanomaterials were screened using the pretreatment process that measured xylanase activity and cell growth. Carbon nanotubes (CNT) and graphene oxide (GO) were analyzed and their immobilization and culture condition factors were optimized using Response Surface Methodology (RSM) with different design models, as well as large-scale production process using a bioreactor. Analysis on the carbon nanomaterial was done using a Field Emission Scanning Electron Microscopy with Energy Dispersive X-ray (FESEM-EDX) and Fourier Transform Infrared spectroscopy (FTIR) while Ultra High-Performance Liquid Chromatography (UHPLC) was used to analyze the final sugar product. The most important factors in xylanase production with low cell leakage are cell loading and agar concentration. Following RSM screening and optimization, the xylanase production from free cells (1.39 U/mL) increased tenfold after cell immobilization (10.30 U/mL), and increased to 15 U/mL during the upscale process in the bioreactor. The immobilized cells can be reused for up to 7 fermentation cycles and stored at 4 ℃ for up to 90 days. The end products of lignocellulosic biomass bioconversion are xylobiose and xylotriose. Cell immobilization with carbon nanomaterials has been shown to successfully enhance xylanase production, opening up a new path to improved bioprocessing, particularly for the production of enzymes with reusability and long-term storage.
format Thesis
author Abdul Manaf, Shoriya Aruni
author_facet Abdul Manaf, Shoriya Aruni
author_sort Abdul Manaf, Shoriya Aruni
title Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_short Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_full Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_fullStr Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_full_unstemmed Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_sort cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
publishDate 2022
url http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf
http://eprints.uthm.edu.my/8486/2/SHORIYA%20ARUNI%20ABDUL%20MANAF%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8486/3/SHORIYA%20ARUNI%20ABDUL%20MANAF%20WATERMARK.pdf
http://eprints.uthm.edu.my/8486/
_version_ 1762394684076851200
score 13.211869

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