Fundamental study of pulse electric field effects on hela cell cultured over extracellular matrix protein micro-patterned surface
Electroporation (EP) is a method of controlling cell function by using pulses of electrical fields to create pores through cell membrane and causes other substance around it to be absorbed into the cell. This method has led to a variety of medical applications, particularly in cell studies. In th...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/845/1/24p%20NUR%20ADILAH%20ABD%20RAHMAN.pdf http://eprints.uthm.edu.my/845/2/NUR%20ADILAH%20ABD%20RAHMAN%20WATERMARK.pdf http://eprints.uthm.edu.my/845/ |
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| Summary: | Electroporation (EP) is a method of controlling cell function by using pulses of
electrical fields to create pores through cell membrane and causes other substance
around it to be absorbed into the cell. This method has led to a variety of medical
applications, particularly in cell studies. In this study, a high voltage of 2 kV/cm with
pulse duration of 30 µs was applied on HeLa cell (human cervical cancer cell) to
investigate the electroporation process. In addition, this study focused on the effect of
protein coated surface, combined with the pulse parameter mentioned above, to look
at its effect on HeLa cell when exposed to high voltage. Thus, will lead towards cell
surface attachment factors interrogation plus the presence of electric field as the
stimulator for an aggressive growth rate of the cells. This was achieved by using the
micro contact printing (μCP) method. The result showed positive respond on the effect
of EP on protein printed surface combination where HeLa cells were grown. The 50µm
was chosen as the best-pattern size for cell alignment by using fibronectin. From the
cell guidance study we could clearly see the cell responses on the protein patterned
surface are much elongated in comparison to the control. In addition, the cells plated
on this patterned surface were further investigated with electroporation technique, in
order to see the effect of electroporation on the cancer cell proliferation and other
cellular activities. The result shows that the cells aligned and elongated on fibronectin
pattern with PEF than without PEF exposure. The combination of these two techniques
will contribute towards understanding the cell surface interface and cell surface
attachment factors which may lead towards a new method for guiding cell towards
wound healing process. |
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