Development of high voltage pulse inducement method for biological cell
Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable high...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/8040/1/P8721_d7eb1fc9936f85924360524c7e73231f.pdf http://eprints.uthm.edu.my/8040/ |
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| Summary: | Electroporation (EP) system is a process of controlling
cell functions by using electromagnetic fields (EMF) to create
pores through a cellular membrane that causes cell lysis and
apoptosis. In this paper we present an experimental setup for
fundamental studies on cell EP. An adjustable high voltage pulse
generator (3kV/10μs – 600μs pulse length) system were connected to
the EP chamber which subsequently allow real time observation
of membrane permeability changes and cellular physiology.
In order to initiate higher cell viability rate, high transfection
efficiency, lower sample contamination and smaller Joule heating
the modification of EP chamber need to be implemented. .
Following that, HeLa cell culture has been projected as cell that
will be used in this study. Finally, some suggestions are proposed
for the future studies. |
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