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Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B

Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, hig...

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Main Author: Sundra, Sargunan
Format: Thesis
Language:English
English
English
Published: 2018
Subjects:
TP Chemical technology
TP155-156 Chemical engineering
Online Access:http://eprints.uthm.edu.my/7829/2/24p%20SARGUNAN%20SUNDRA.pdf
http://eprints.uthm.edu.my/7829/1/SARGUNAN%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/7829/3/SARGUNAN%20SUNDRA%20WATERMARK.pdf
http://eprints.uthm.edu.my/7829/
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spelling my.uthm.eprints.78292022-10-12T02:23:37Z http://eprints.uthm.edu.my/7829/ Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B Sundra, Sargunan TP Chemical technology TP155-156 Chemical engineering Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, higher complexity procedures and costly. Micromixing method provides a better platfonn that enables mixing and dilution of liquid-based reagents which is convenient solutions preparation, easy liquid handling and time-saving. In this study, a polydimethylsiloxane (PDMS) micromixer was designed, simulated and prototyped using vinyl tape method and successfully applied to mix and dilute Cytochalasin-B in culture media (CB-DMEM, 30.0 µM) with 0.05 % ethanol solutions (diluent) to produce four different concentrations of CB-DMEM (5.3, I 0.6, 14.8, and 20.2 µM). The different concentrations of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking technique. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were assessed via phase contrast microscopy, live/dead staining and Alamar Blue® staining respectively. The results show that both 2D and 30 of ORL-48 microtissues were only morphologically affected (fibroblastic spreading to round shape) while cell viability and cell proliferation show that CB treatment solely does not causes apoptosis (:::::: 90 % cells are alive and able to proliferate). The micromixer employed in solution preparation of CB-DMEM (5.3, 10.6, 14.8, and 20.2 2018-06 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/7829/2/24p%20SARGUNAN%20SUNDRA.pdf text en http://eprints.uthm.edu.my/7829/1/SARGUNAN%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf text en http://eprints.uthm.edu.my/7829/3/SARGUNAN%20SUNDRA%20WATERMARK.pdf Sundra, Sargunan (2018) Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B. Masters thesis, Universiti Tun Hussein Onn Malaysia.
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
English
English
topic TP Chemical technology
TP155-156 Chemical engineering
spellingShingle TP Chemical technology
TP155-156 Chemical engineering
Sundra, Sargunan
Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
description Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, higher complexity procedures and costly. Micromixing method provides a better platfonn that enables mixing and dilution of liquid-based reagents which is convenient solutions preparation, easy liquid handling and time-saving. In this study, a polydimethylsiloxane (PDMS) micromixer was designed, simulated and prototyped using vinyl tape method and successfully applied to mix and dilute Cytochalasin-B in culture media (CB-DMEM, 30.0 µM) with 0.05 % ethanol solutions (diluent) to produce four different concentrations of CB-DMEM (5.3, I 0.6, 14.8, and 20.2 µM). The different concentrations of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking technique. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were assessed via phase contrast microscopy, live/dead staining and Alamar Blue® staining respectively. The results show that both 2D and 30 of ORL-48 microtissues were only morphologically affected (fibroblastic spreading to round shape) while cell viability and cell proliferation show that CB treatment solely does not causes apoptosis (:::::: 90 % cells are alive and able to proliferate). The micromixer employed in solution preparation of CB-DMEM (5.3, 10.6, 14.8, and 20.2
format Thesis
author Sundra, Sargunan
author_facet Sundra, Sargunan
author_sort Sundra, Sargunan
title Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_short Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_full Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_fullStr Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_full_unstemmed Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_sort development of a micromixing system for treatment of orl 48 microtissues in different concentrations of cytochalasin b
publishDate 2018
url http://eprints.uthm.edu.my/7829/2/24p%20SARGUNAN%20SUNDRA.pdf
http://eprints.uthm.edu.my/7829/1/SARGUNAN%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/7829/3/SARGUNAN%20SUNDRA%20WATERMARK.pdf
http://eprints.uthm.edu.my/7829/
_version_ 1748182662329139200
score 13.211869

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