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Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene

Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was cond...

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Main Authors: Kamarudin, Kamarul Rahim, Mohamed Rehan, Maryam, Mohamed Rehan, Aisyah
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2018
Subjects:
HF5001-6182 Business
Online Access:http://eprints.uthm.edu.my/5919/1/AJ%202018%20%28659%29.pdf
http://eprints.uthm.edu.my/5919/
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spelling my.uthm.eprints.59192022-01-25T00:23:01Z http://eprints.uthm.edu.my/5919/ Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah HF5001-6182 Business Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was conducted to determine the species identities of processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples for species identification, and five fresh additional specimens from Teluk Nipah Beach, Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens, Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising family Holothuriidae (Holothuria (Mertensiothuria) leucospilota, Holothuria (Metriatyla)scabra, Holothuria (Metriatyla) lessoni, Holothuria (Halodeima) atra, and Holothuria (Halodeima) edulis) and family Caudinidae (Acaudina molpadioides). The outcomes of this study also highlighted the availability of 40 new 12S mitochondrial rRNA gene sequences deposited in the GenBank that can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets. Universiti Putra Malaysia Press 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/5919/1/AJ%202018%20%28659%29.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2018) Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene. Tropical Agricultural Science, 41 (4). pp. 1833-1851. ISSN 2231-8542
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic HF5001-6182 Business
spellingShingle HF5001-6182 Business
Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
description Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was conducted to determine the species identities of processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples for species identification, and five fresh additional specimens from Teluk Nipah Beach, Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens, Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising family Holothuriidae (Holothuria (Mertensiothuria) leucospilota, Holothuria (Metriatyla)scabra, Holothuria (Metriatyla) lessoni, Holothuria (Halodeima) atra, and Holothuria (Halodeima) edulis) and family Caudinidae (Acaudina molpadioides). The outcomes of this study also highlighted the availability of 40 new 12S mitochondrial rRNA gene sequences deposited in the GenBank that can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.
format Article
author Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
author_facet Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
author_sort Kamarudin, Kamarul Rahim
title Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
title_short Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
title_full Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
title_fullStr Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
title_full_unstemmed Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene
title_sort species identification and molecular phylogenetics of processed sea cucumbers from malaysian market based on 12s mitochondrial rrna gene
publisher Universiti Putra Malaysia Press
publishDate 2018
url http://eprints.uthm.edu.my/5919/1/AJ%202018%20%28659%29.pdf
http://eprints.uthm.edu.my/5919/
_version_ 1738581431100112896
score 13.211869

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