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Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences

This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothu...

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Main Authors: Kamarudin, Kamarul Rahim, Mohamed Rehan, ‘Aisyah, Hashim, Ridzwan, Usup, Gires, Mohamed Rehan, Maryam
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2016
Subjects:
TA164 Bioengineering
Online Access:http://eprints.uthm.edu.my/5651/1/AJ%202016%20%28105%29.pdf
http://eprints.uthm.edu.my/5651/
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spelling my.uthm.eprints.56512022-01-19T08:34:00Z http://eprints.uthm.edu.my/5651/ Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences Kamarudin, Kamarul Rahim Mohamed Rehan, ‘Aisyah Hashim, Ridzwan Usup, Gires Mohamed Rehan, Maryam TA164 Bioengineering This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothuria species into its current subgenera. The undetermined species clustered with all taxa of Holothuria in previous study. Phylogenetic analyses using maximum parsimony and Bayesian methods suggest that the undetermined species was genetically closer to Holothuria (Lessonothuria) pardalis and Holothuria (Acanthotrapeza) coluber; and its position in both phylogenetic trees further suggests its status as a Holothuria taxon. Subgenera of Holothuria, Merthensiothuria and Metriatyla are monophyletic with strong bootstrap supports and posterior probabilities of clades, thus strengthening their morphological taxonomies. Nonetheless, the non-monophyly of subgenera of Halodeima, Microthele and Platyperona suggests a requirement for their taxonomic revisions using integrative taxonomy. The status of Holothuria (Halodeima) edulis subgroups in the maximum parsimony and Bayesian trees is indistinct and further taxonomic revisions are necessary. In terms of sister relationship, both phylogenetic trees suggest that subgenus Holothuria is a sister taxon of subgenus Roweothuria while the other sister relationships were unclear due to the undetermined species, paraphyly and polyphyly of a number of subgenera. Further studies with more specimens of genus Holothuria from broader geographical locations and various mtDNA genes along with morphological approaches may facilitate to provide better insights into the molecular phylogeny of subgenera of Holothuria. Universiti Kebangsaan Malaysia 2016 Article PeerReviewed text en http://eprints.uthm.edu.my/5651/1/AJ%202016%20%28105%29.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, ‘Aisyah and Hashim, Ridzwan and Usup, Gires and Mohamed Rehan, Maryam (2016) Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences. Sains Malaysiana, 45 (7). pp. 1079-1087. ISSN 0126-6039
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Kamarudin, Kamarul Rahim
Mohamed Rehan, ‘Aisyah
Hashim, Ridzwan
Usup, Gires
Mohamed Rehan, Maryam
Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
description This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothuria species into its current subgenera. The undetermined species clustered with all taxa of Holothuria in previous study. Phylogenetic analyses using maximum parsimony and Bayesian methods suggest that the undetermined species was genetically closer to Holothuria (Lessonothuria) pardalis and Holothuria (Acanthotrapeza) coluber; and its position in both phylogenetic trees further suggests its status as a Holothuria taxon. Subgenera of Holothuria, Merthensiothuria and Metriatyla are monophyletic with strong bootstrap supports and posterior probabilities of clades, thus strengthening their morphological taxonomies. Nonetheless, the non-monophyly of subgenera of Halodeima, Microthele and Platyperona suggests a requirement for their taxonomic revisions using integrative taxonomy. The status of Holothuria (Halodeima) edulis subgroups in the maximum parsimony and Bayesian trees is indistinct and further taxonomic revisions are necessary. In terms of sister relationship, both phylogenetic trees suggest that subgenus Holothuria is a sister taxon of subgenus Roweothuria while the other sister relationships were unclear due to the undetermined species, paraphyly and polyphyly of a number of subgenera. Further studies with more specimens of genus Holothuria from broader geographical locations and various mtDNA genes along with morphological approaches may facilitate to provide better insights into the molecular phylogeny of subgenera of Holothuria.
format Article
author Kamarudin, Kamarul Rahim
Mohamed Rehan, ‘Aisyah
Hashim, Ridzwan
Usup, Gires
Mohamed Rehan, Maryam
author_facet Kamarudin, Kamarul Rahim
Mohamed Rehan, ‘Aisyah
Hashim, Ridzwan
Usup, Gires
Mohamed Rehan, Maryam
author_sort Kamarudin, Kamarul Rahim
title Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
title_short Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
title_full Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
title_fullStr Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
title_full_unstemmed Phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rRNA gene sequences
title_sort phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rrna gene sequences
publisher Universiti Kebangsaan Malaysia
publishDate 2016
url http://eprints.uthm.edu.my/5651/1/AJ%202016%20%28105%29.pdf
http://eprints.uthm.edu.my/5651/
_version_ 1738581401531318272
score 13.211869

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