Staff View: Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships...

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Main Authors:	Md-Zain, Badrul Munir, Abdul-Mutalib, Shahidatina Aishah, Aifat, Nor Rahman, Masstor, Noor Haslina, Mohd-Yusuf, Nur Syafika, Mohd-Hashim, Abu, Abdul-Latiff, Muhammad Abu Bakar, Yaakop, Salmah, Samat, Abdullah
Format:	Article
Language:	English
Published:	Society for Indonesian Biodiversity 2018
Subjects:	SH Aquaculture. Fisheries. Angling SH328-329 Fishery management. Fishery policy
Online Access:	http://eprints.uthm.edu.my/5648/1/AJ%202018%20%28286%29.pdf http://eprints.uthm.edu.my/5648/
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spelling	my.uthm.eprints.56482022-01-19T08:14:47Z http://eprints.uthm.edu.my/5648/ Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets Md-Zain, Badrul Munir Abdul-Mutalib, Shahidatina Aishah Aifat, Nor Rahman Masstor, Noor Haslina Mohd-Yusuf, Nur Syafika Mohd-Hashim, Abu Abdul-Latiff, Muhammad Abu Bakar Yaakop, Salmah Samat, Abdullah SH Aquaculture. Fisheries. Angling SH328-329 Fishery management. Fishery policy The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships of white-spotted guitarfish, which portray a similar morphology to sharks and rays. The main objective of this study was to determine the phylogenetic position of R. australiae using cytochrome oxidase I (COI) sequences of mitochondrial DNA based on fish samples collected from local Malaysian fish markets. This study included nine genetic samples of R. australiae and fourteen samples from other members of the shark and ray families, including Sphyrna lewini (Sphyrnidae), Rhizoprionodon oligolinx and Carcharhinus sorrah (Carcharhinidae), Dasyatis zugei, Himantura walga, Himantura gerradi, Himantura jenkinsii and Neotrygon kuhlii (Dasyatidae). Chimaera fulva, a member of the Chimaera family, was used as the outgroup. Sequences in size of ~701 base pairs were successfully obtained from all fish samples. The phylogenetic tree topology was reconstructed using distance-based (neighbor-joining) and character-based (maximum parsimony) methods using MEGA and PAUP software. Results indicated that R. australiae formed monophyletic clade and is closely related to sharks (Sphyrnidae and Carcharhinidae). This conclusion was also supported by genetic distance analysis which indicated that Rhynchobatidae and sharks (Carcharhinidae and Sphyrnidae) were closer to each other than to rays (Dasyatidae). This study has proven the efficiency of the COI mitochondrial locus in revealing the phylogenetic position of R. australiae. Research findings from this study have increased our understanding of the phylogenetic relationships among guitarfish, sharks, and rays, and their respective taxonomic positions are given their shared morphological characters. This will benefit us in identifying these fish species before consumption from local fish markets. Society for Indonesian Biodiversity 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/5648/1/AJ%202018%20%28286%29.pdf Md-Zain, Badrul Munir and Abdul-Mutalib, Shahidatina Aishah and Aifat, Nor Rahman and Masstor, Noor Haslina and Mohd-Yusuf, Nur Syafika and Mohd-Hashim, Abu and Abdul-Latiff, Muhammad Abu Bakar and Yaakop, Salmah and Samat, Abdullah (2018) Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. Biodiversitas Journal of Biological Diversity, 19 (4). pp. 1382-1386. ISSN 1412-033X
institution	Universiti Tun Hussein Onn Malaysia
building	UTHM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Tun Hussein Onn Malaysia
content_source	UTHM Institutional Repository
url_provider	http://eprints.uthm.edu.my/
language	English
topic	SH Aquaculture. Fisheries. Angling SH328-329 Fishery management. Fishery policy
spellingShingle	SH Aquaculture. Fisheries. Angling SH328-329 Fishery management. Fishery policy Md-Zain, Badrul Munir Abdul-Mutalib, Shahidatina Aishah Aifat, Nor Rahman Masstor, Noor Haslina Mohd-Yusuf, Nur Syafika Mohd-Hashim, Abu Abdul-Latiff, Muhammad Abu Bakar Yaakop, Salmah Samat, Abdullah Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
description	The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships of white-spotted guitarfish, which portray a similar morphology to sharks and rays. The main objective of this study was to determine the phylogenetic position of R. australiae using cytochrome oxidase I (COI) sequences of mitochondrial DNA based on fish samples collected from local Malaysian fish markets. This study included nine genetic samples of R. australiae and fourteen samples from other members of the shark and ray families, including Sphyrna lewini (Sphyrnidae), Rhizoprionodon oligolinx and Carcharhinus sorrah (Carcharhinidae), Dasyatis zugei, Himantura walga, Himantura gerradi, Himantura jenkinsii and Neotrygon kuhlii (Dasyatidae). Chimaera fulva, a member of the Chimaera family, was used as the outgroup. Sequences in size of ~701 base pairs were successfully obtained from all fish samples. The phylogenetic tree topology was reconstructed using distance-based (neighbor-joining) and character-based (maximum parsimony) methods using MEGA and PAUP software. Results indicated that R. australiae formed monophyletic clade and is closely related to sharks (Sphyrnidae and Carcharhinidae). This conclusion was also supported by genetic distance analysis which indicated that Rhynchobatidae and sharks (Carcharhinidae and Sphyrnidae) were closer to each other than to rays (Dasyatidae). This study has proven the efficiency of the COI mitochondrial locus in revealing the phylogenetic position of R. australiae. Research findings from this study have increased our understanding of the phylogenetic relationships among guitarfish, sharks, and rays, and their respective taxonomic positions are given their shared morphological characters. This will benefit us in identifying these fish species before consumption from local fish markets.
format	Article
author	Md-Zain, Badrul Munir Abdul-Mutalib, Shahidatina Aishah Aifat, Nor Rahman Masstor, Noor Haslina Mohd-Yusuf, Nur Syafika Mohd-Hashim, Abu Abdul-Latiff, Muhammad Abu Bakar Yaakop, Salmah Samat, Abdullah
author_facet	Md-Zain, Badrul Munir Abdul-Mutalib, Shahidatina Aishah Aifat, Nor Rahman Masstor, Noor Haslina Mohd-Yusuf, Nur Syafika Mohd-Hashim, Abu Abdul-Latiff, Muhammad Abu Bakar Yaakop, Salmah Samat, Abdullah
author_sort	Md-Zain, Badrul Munir
title	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_short	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_full	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_fullStr	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_full_unstemmed	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_sort	molecular phylogenetic inference of white-spotted guitarfish (rhynchobatus australiae) collected from local malaysian fish markets
publisher	Society for Indonesian Biodiversity
publishDate	2018
url	http://eprints.uthm.edu.my/5648/1/AJ%202018%20%28286%29.pdf http://eprints.uthm.edu.my/5648/
_version_	1738581401121325056
score	13.211869

Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

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