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In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48...

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Main Authors: Leong, Wai Yean, Soon, Chin Fhong, Wong, Soon Chuan, Tee, Kian Sek, Cheong, Sok Ching, Gan, Siew Hua, Youseffi, Mansour
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2017
Subjects:
QD Chemistry
Online Access:http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf
http://eprints.uthm.edu.my/3700/
http://dx.doi.org/10.3390/bioengineering4020043
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spelling my.uthm.eprints.37002021-11-21T07:50:44Z http://eprints.uthm.edu.my/3700/ In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique Leong, Wai Yean Soon, Chin Fhong Wong, Soon Chuan Tee, Kian Sek Cheong, Sok Ching Gan, Siew Hua Youseffi, Mansour QD Chemistry Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 µL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 µm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. Multidisciplinary Digital Publishing Institute 2017 Article PeerReviewed text en http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf Leong, Wai Yean and Soon, Chin Fhong and Wong, Soon Chuan and Tee, Kian Sek and Cheong, Sok Ching and Gan, Siew Hua and Youseffi, Mansour (2017) In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique. Bioengineering, 4 (45). pp. 1-14. ISSN 2306-5354 http://dx.doi.org/10.3390/bioengineering4020043
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Leong, Wai Yean
Soon, Chin Fhong
Wong, Soon Chuan
Tee, Kian Sek
Cheong, Sok Ching
Gan, Siew Hua
Youseffi, Mansour
In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
description Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 µL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 µm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.
format Article
author Leong, Wai Yean
Soon, Chin Fhong
Wong, Soon Chuan
Tee, Kian Sek
Cheong, Sok Ching
Gan, Siew Hua
Youseffi, Mansour
author_facet Leong, Wai Yean
Soon, Chin Fhong
Wong, Soon Chuan
Tee, Kian Sek
Cheong, Sok Ching
Gan, Siew Hua
Youseffi, Mansour
author_sort Leong, Wai Yean
title In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
title_short In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
title_full In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
title_fullStr In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
title_full_unstemmed In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
title_sort in vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
publisher Multidisciplinary Digital Publishing Institute
publishDate 2017
url http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf
http://eprints.uthm.edu.my/3700/
http://dx.doi.org/10.3390/bioengineering4020043
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score 13.211869

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