Staff View: Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting

Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting

Postoperative bleeding following cardiac surgeries is still an issue that deranges the medical resources and cost. The oral and injection administrations of blood coagulation protein, Factor VII (FVII), is effective to stop the bleeding. However, its short half-life has limited the effectiveness of...

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Main Authors:	Rathosivan Gopal, Rathosivan Gopal, Md Shakhih, Muhammad Faiz, Mariaulpa Sahalan, Mariaulpa Sahalan, Te Chuan Lee, Te Chuan Lee, Hendra Hermawan, Hendra Hermawan, Sivakumar Sivalingam, Sivakumar Sivalingam, Kadiman, Suhaini, Saidin, Syafiqah
Format:	Article
Language:	English
Published:	Elsevier 2023
Subjects:	TP248.13-248.65 Biotechnology
Online Access:	http://eprints.uthm.edu.my/10094/1/J16187_a1d83041e2470ffe3771a93396a5cc32.pdf http://eprints.uthm.edu.my/10094/ https://doi.org/10.1016/j.colsurfb.2023.113390
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id	my.uthm.eprints.10094
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spelling	my.uthm.eprints.100942023-10-11T07:31:35Z http://eprints.uthm.edu.my/10094/ Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting Rathosivan Gopal, Rathosivan Gopal Md Shakhih, Muhammad Faiz Mariaulpa Sahalan, Mariaulpa Sahalan Te Chuan Lee, Te Chuan Lee Hendra Hermawan, Hendra Hermawan Sivakumar Sivalingam, Sivakumar Sivalingam Kadiman, Suhaini Saidin, Syafiqah TP248.13-248.65 Biotechnology Postoperative bleeding following cardiac surgeries is still an issue that deranges the medical resources and cost. The oral and injection administrations of blood coagulation protein, Factor VII (FVII), is effective to stop the bleeding. However, its short half-life has limited the effectiveness of this treatment and frequent FVII intake may distress the patients. Instead, incorporating FVII into synthetic biodegradable polymers such as polycaprolactone (PCL) that is commonly used in drug delivery applications should provide a solution. Therefore, this study aimed to immobilize FVII on PCL membranes through a cross-linkage polydopamine (PDA) grafting as an intermediate layer. These membranes are intended to provide a solution for cardiac bleeding in coagulating blood and sealing the sutured region. The membranes were evaluated in terms of its physio-chemical properties, thermal behavior, FVII release profile and biocompatibility properties. The ATR-FTIR was used to analyze the chemical functionalities of the membranes. Further validation was done with XPS where the appearances of 0.45 ± 0.06% sulfur composition and C–S peak have confirmed the immobilization of FVII on the PCL membranes. The cross-linked FVIIs were viewed in spherical immobilization on the PCL membranes with a size range between 30 and 210 nm. The surface roughness and hydrophilicity of the membranes were enhanced with a slight shift of melting temperature. The PCL-PDA-FVII0.03 and PCL-PDA-FVII0.05 membranes, with wide area of FVII immobilization released approximately only 22% of FVII into the solution within 60 days period and, it is found that the PCLPDA-FVIIx membranes projected the Higuchi release model with non-Fickian anomalous transport. While the cytotoxic and hemocompatibility analyses showed advance cell viability, identical coagulation time and low hemolysis ratio on the PCL-PDA-FVIIx membranes. The erythrocytes were viewed in polyhedrocyte coagulated structure under SEM visualization. These results validate the biocompatibility of the membranes and its ability to prolong blood coagulation, thus highlighting its potential application as cardiac bleeding sealant. Elsevier 2023 Article PeerReviewed text en http://eprints.uthm.edu.my/10094/1/J16187_a1d83041e2470ffe3771a93396a5cc32.pdf Rathosivan Gopal, Rathosivan Gopal and Md Shakhih, Muhammad Faiz and Mariaulpa Sahalan, Mariaulpa Sahalan and Te Chuan Lee, Te Chuan Lee and Hendra Hermawan, Hendra Hermawan and Sivakumar Sivalingam, Sivakumar Sivalingam and Kadiman, Suhaini and Saidin, Syafiqah (2023) Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting. Colloids and Surfaces B: Biointerfaces, 228. pp. 1-14. https://doi.org/10.1016/j.colsurfb.2023.113390
institution	Universiti Tun Hussein Onn Malaysia
building	UTHM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Tun Hussein Onn Malaysia
content_source	UTHM Institutional Repository
url_provider	http://eprints.uthm.edu.my/
language	English
topic	TP248.13-248.65 Biotechnology
spellingShingle	TP248.13-248.65 Biotechnology Rathosivan Gopal, Rathosivan Gopal Md Shakhih, Muhammad Faiz Mariaulpa Sahalan, Mariaulpa Sahalan Te Chuan Lee, Te Chuan Lee Hendra Hermawan, Hendra Hermawan Sivakumar Sivalingam, Sivakumar Sivalingam Kadiman, Suhaini Saidin, Syafiqah Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
description	Postoperative bleeding following cardiac surgeries is still an issue that deranges the medical resources and cost. The oral and injection administrations of blood coagulation protein, Factor VII (FVII), is effective to stop the bleeding. However, its short half-life has limited the effectiveness of this treatment and frequent FVII intake may distress the patients. Instead, incorporating FVII into synthetic biodegradable polymers such as polycaprolactone (PCL) that is commonly used in drug delivery applications should provide a solution. Therefore, this study aimed to immobilize FVII on PCL membranes through a cross-linkage polydopamine (PDA) grafting as an intermediate layer. These membranes are intended to provide a solution for cardiac bleeding in coagulating blood and sealing the sutured region. The membranes were evaluated in terms of its physio-chemical properties, thermal behavior, FVII release profile and biocompatibility properties. The ATR-FTIR was used to analyze the chemical functionalities of the membranes. Further validation was done with XPS where the appearances of 0.45 ± 0.06% sulfur composition and C–S peak have confirmed the immobilization of FVII on the PCL membranes. The cross-linked FVIIs were viewed in spherical immobilization on the PCL membranes with a size range between 30 and 210 nm. The surface roughness and hydrophilicity of the membranes were enhanced with a slight shift of melting temperature. The PCL-PDA-FVII0.03 and PCL-PDA-FVII0.05 membranes, with wide area of FVII immobilization released approximately only 22% of FVII into the solution within 60 days period and, it is found that the PCLPDA-FVIIx membranes projected the Higuchi release model with non-Fickian anomalous transport. While the cytotoxic and hemocompatibility analyses showed advance cell viability, identical coagulation time and low hemolysis ratio on the PCL-PDA-FVIIx membranes. The erythrocytes were viewed in polyhedrocyte coagulated structure under SEM visualization. These results validate the biocompatibility of the membranes and its ability to prolong blood coagulation, thus highlighting its potential application as cardiac bleeding sealant.
format	Article
author	Rathosivan Gopal, Rathosivan Gopal Md Shakhih, Muhammad Faiz Mariaulpa Sahalan, Mariaulpa Sahalan Te Chuan Lee, Te Chuan Lee Hendra Hermawan, Hendra Hermawan Sivakumar Sivalingam, Sivakumar Sivalingam Kadiman, Suhaini Saidin, Syafiqah
author_facet	Rathosivan Gopal, Rathosivan Gopal Md Shakhih, Muhammad Faiz Mariaulpa Sahalan, Mariaulpa Sahalan Te Chuan Lee, Te Chuan Lee Hendra Hermawan, Hendra Hermawan Sivakumar Sivalingam, Sivakumar Sivalingam Kadiman, Suhaini Saidin, Syafiqah
author_sort	Rathosivan Gopal, Rathosivan Gopal
title	Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
title_short	Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
title_full	Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
title_fullStr	Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
title_full_unstemmed	Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting
title_sort	immobilization of blood coagulant factor vii on polycaprolactone membrane through polydopamine grafting
publisher	Elsevier
publishDate	2023
url	http://eprints.uthm.edu.my/10094/1/J16187_a1d83041e2470ffe3771a93396a5cc32.pdf http://eprints.uthm.edu.my/10094/ https://doi.org/10.1016/j.colsurfb.2023.113390
_version_	1781707432899117056
score	13.211869

Immobilization of blood coagulant factor VII on polycaprolactone membrane through polydopamine grafting

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