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Quality assurance of vital® test meal in gastric emptying scintigraphy

In vivo studies of gastric emptying scintigraphy (GES) had demonstrated that the high-calorie drink, Vital® could serve as an alternative to people who cannot tolerate the conventional solid egg-white meal (EWM). Further evaluations were needed to ensure its reliability as a liquid meal for GES. The...

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Main Author: Wen, Ngo Hui
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2024
Subjects:
R Medicine
RC71-78.7 Examination. Diagnosis Including raiography
Online Access:http://eprints.usm.my/61362/1/Ngo%20Hui%20Wen-E.pdf
http://eprints.usm.my/61362/
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spelling my.usm.eprints.61362 http://eprints.usm.my/61362/ Quality assurance of vital® test meal in gastric emptying scintigraphy Wen, Ngo Hui R Medicine RC71-78.7 Examination. Diagnosis Including raiography In vivo studies of gastric emptying scintigraphy (GES) had demonstrated that the high-calorie drink, Vital® could serve as an alternative to people who cannot tolerate the conventional solid egg-white meal (EWM). Further evaluations were needed to ensure its reliability as a liquid meal for GES. Therefore, we sought to address two quality assurance aspects to validate Vital®, which included uniform dispersion of the radiotracer and in vitro radiolabelling efficiency and stability. Methods: A simple mixing method was proposed where 99mTc-phytate (99mTc-P) was injected into the Vital® drink without removing the aluminium seal, and it was gently swirled for a minute. The drink was then imaged for 15 minutes using dynamic scintigraphy and an analysis of the regions of interest (ROI) at the top and bottom of the of the drink was done. For the radiolabelling efficiency and stability experiment, 12 samples of Vital® were mixed with 99mTc-P, incubated with human saliva supernatant and gastric fluid for 4 hours, then centrifuged and filtered at hourly intervals. The radioactivity of the solid part and filtrate was assessed using 1-minute static imaging. Findings: Results showed no significant differences in activity percentages between the top and bottom of the drink over 15 minutes (p >0.05), suggesting a uniform distribution of the radiotracer throughout the drink. Significantly more radioactivity was found in the filtrate compared to the solid part (p <0.05), indicating a preferential radiolabelling of the aqueous phase of Vital®. The radiotracer remained stable in both the solid and aqueous phases of Vital® in the simulated gastric environment over 2-4 hours, with no significant differences observed between each time point (p >0.05). Conclusions: This study offered detailed insights into how the high- calorie Vital® nutrient drink behaves, improving the understanding of previous GES findings. It could serve as a key reference for future research and clinical practices in choosing and preparing liquid test meals, leading to more accurate GES results. Universiti Sains Malaysia 2024-07 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/61362/1/Ngo%20Hui%20Wen-E.pdf Wen, Ngo Hui (2024) Quality assurance of vital® test meal in gastric emptying scintigraphy. Project Report. Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
RC71-78.7 Examination. Diagnosis Including raiography
spellingShingle R Medicine
RC71-78.7 Examination. Diagnosis Including raiography
Wen, Ngo Hui
Quality assurance of vital® test meal in gastric emptying scintigraphy
description In vivo studies of gastric emptying scintigraphy (GES) had demonstrated that the high-calorie drink, Vital® could serve as an alternative to people who cannot tolerate the conventional solid egg-white meal (EWM). Further evaluations were needed to ensure its reliability as a liquid meal for GES. Therefore, we sought to address two quality assurance aspects to validate Vital®, which included uniform dispersion of the radiotracer and in vitro radiolabelling efficiency and stability. Methods: A simple mixing method was proposed where 99mTc-phytate (99mTc-P) was injected into the Vital® drink without removing the aluminium seal, and it was gently swirled for a minute. The drink was then imaged for 15 minutes using dynamic scintigraphy and an analysis of the regions of interest (ROI) at the top and bottom of the of the drink was done. For the radiolabelling efficiency and stability experiment, 12 samples of Vital® were mixed with 99mTc-P, incubated with human saliva supernatant and gastric fluid for 4 hours, then centrifuged and filtered at hourly intervals. The radioactivity of the solid part and filtrate was assessed using 1-minute static imaging. Findings: Results showed no significant differences in activity percentages between the top and bottom of the drink over 15 minutes (p >0.05), suggesting a uniform distribution of the radiotracer throughout the drink. Significantly more radioactivity was found in the filtrate compared to the solid part (p <0.05), indicating a preferential radiolabelling of the aqueous phase of Vital®. The radiotracer remained stable in both the solid and aqueous phases of Vital® in the simulated gastric environment over 2-4 hours, with no significant differences observed between each time point (p >0.05). Conclusions: This study offered detailed insights into how the high- calorie Vital® nutrient drink behaves, improving the understanding of previous GES findings. It could serve as a key reference for future research and clinical practices in choosing and preparing liquid test meals, leading to more accurate GES results.
format Monograph
author Wen, Ngo Hui
author_facet Wen, Ngo Hui
author_sort Wen, Ngo Hui
title Quality assurance of vital® test meal in gastric emptying scintigraphy
title_short Quality assurance of vital® test meal in gastric emptying scintigraphy
title_full Quality assurance of vital® test meal in gastric emptying scintigraphy
title_fullStr Quality assurance of vital® test meal in gastric emptying scintigraphy
title_full_unstemmed Quality assurance of vital® test meal in gastric emptying scintigraphy
title_sort quality assurance of vital® test meal in gastric emptying scintigraphy
publisher Universiti Sains Malaysia
publishDate 2024
url http://eprints.usm.my/61362/1/Ngo%20Hui%20Wen-E.pdf
http://eprints.usm.my/61362/
_version_ 1821002642606784512
score 13.23648

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