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The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study

The disruption of gut dysbiosis after spinal cord injury (SCI) is a crucial but poorly understood phenomenon that might affect SCI healing. This study addresses the need to examine locomotor recovery after SCI and presents an animal model that may help restore a healthy gut flora and improve SCI the...

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Main Author: Manickam, Danniya Lakshmi
Format: Thesis
Language:English
Published: 2023
Subjects:
R Medicine
RA440-440.87 Study and teaching. Research
Online Access:http://eprints.usm.my/61061/1/Danniya%20Lakshmi%20Manickam-E.pdf
http://eprints.usm.my/61061/
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spelling my.usm.eprints.61061 http://eprints.usm.my/61061/ The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study Manickam, Danniya Lakshmi R Medicine RA440-440.87 Study and teaching. Research The disruption of gut dysbiosis after spinal cord injury (SCI) is a crucial but poorly understood phenomenon that might affect SCI healing. This study addresses the need to examine locomotor recovery after SCI and presents an animal model that may help restore a healthy gut flora and improve SCI therapies. The primary objective is to create a reliable animal model of SCI with gut dysbiosis to test probiotic treatments. With a 10% dropout rate (1 additional rat), this study included 13 rats. Adult female Wistar rats were carefully weighed and randomly allocated to one of four groups: Group 1 (control, n = 3), Group 2 (control with antibiotic intake, n = 4), Group 3 (SCI with antibiotic intake, n = 3), and Group 4 (SCI with antibiotic intake and probiotic therapy, n = 3). A rod was dropped from 25 mm to cause a moderate contusion injury in the T9/T10 thoracic area after a laminectomy. Successful surgery was established by the rats' hindlimb paralysis. Five days before SCI, rats received antibiotic mixtures to produce intestinal dysbiosis. Their drinking water included 2 g/L streptomycin, 0.17 g/L gentamicin, 0.125 mg/L ciprofloxacin, and 1 g/L bacitracin. After surgery, the rats returned to their usual drinking water. Multi-strain probiotic therapy began on the thirteenth day post-SCI and lasted until the eighteenth. Oral forced feeding of 3g of 30 x 109 CFU/kg probiotics in sterile water was performed. Post-injury faeces were tested for gut dysbiosis on days 4, 11, 18, and 25. In addition to faecal sample analysis, the Basso Beattie Bresnahan Locomotor Rating Scale was used to evaluate the rats' behavioural and locomotor recovery on the 4th, 11th, 18th, and 25th dpi for 4 minutes per rat. After six days of probiotic therapy, faecal sample morphology and BBB locomotor assessments showed a mild effect. Overall, this study sheds light on the significant connection between SCI, intestinal dysbiosis, and probiotics. The study found that probiotics may help SCI patients recover locomotion and gut eubiosis. More study is needed to determine the full range of probiotic benefits. This research reveals new ways to improve SCI patients' results and quality of life. 2023-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/61061/1/Danniya%20Lakshmi%20Manickam-E.pdf Manickam, Danniya Lakshmi (2023) The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
RA440-440.87 Study and teaching. Research
spellingShingle R Medicine
RA440-440.87 Study and teaching. Research
Manickam, Danniya Lakshmi
The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
description The disruption of gut dysbiosis after spinal cord injury (SCI) is a crucial but poorly understood phenomenon that might affect SCI healing. This study addresses the need to examine locomotor recovery after SCI and presents an animal model that may help restore a healthy gut flora and improve SCI therapies. The primary objective is to create a reliable animal model of SCI with gut dysbiosis to test probiotic treatments. With a 10% dropout rate (1 additional rat), this study included 13 rats. Adult female Wistar rats were carefully weighed and randomly allocated to one of four groups: Group 1 (control, n = 3), Group 2 (control with antibiotic intake, n = 4), Group 3 (SCI with antibiotic intake, n = 3), and Group 4 (SCI with antibiotic intake and probiotic therapy, n = 3). A rod was dropped from 25 mm to cause a moderate contusion injury in the T9/T10 thoracic area after a laminectomy. Successful surgery was established by the rats' hindlimb paralysis. Five days before SCI, rats received antibiotic mixtures to produce intestinal dysbiosis. Their drinking water included 2 g/L streptomycin, 0.17 g/L gentamicin, 0.125 mg/L ciprofloxacin, and 1 g/L bacitracin. After surgery, the rats returned to their usual drinking water. Multi-strain probiotic therapy began on the thirteenth day post-SCI and lasted until the eighteenth. Oral forced feeding of 3g of 30 x 109 CFU/kg probiotics in sterile water was performed. Post-injury faeces were tested for gut dysbiosis on days 4, 11, 18, and 25. In addition to faecal sample analysis, the Basso Beattie Bresnahan Locomotor Rating Scale was used to evaluate the rats' behavioural and locomotor recovery on the 4th, 11th, 18th, and 25th dpi for 4 minutes per rat. After six days of probiotic therapy, faecal sample morphology and BBB locomotor assessments showed a mild effect. Overall, this study sheds light on the significant connection between SCI, intestinal dysbiosis, and probiotics. The study found that probiotics may help SCI patients recover locomotion and gut eubiosis. More study is needed to determine the full range of probiotic benefits. This research reveals new ways to improve SCI patients' results and quality of life.
format Thesis
author Manickam, Danniya Lakshmi
author_facet Manickam, Danniya Lakshmi
author_sort Manickam, Danniya Lakshmi
title The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
title_short The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
title_full The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
title_fullStr The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
title_full_unstemmed The role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
title_sort role of probiotics with lactic acid-producing bacteria in modulating the behaviour of rodent model following contusive spinal cord injury: preliminary study
publishDate 2023
url http://eprints.usm.my/61061/1/Danniya%20Lakshmi%20Manickam-E.pdf
http://eprints.usm.my/61061/
_version_ 1814048424646934528
score 13.211869

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