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Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation

Banana peels extracts have been used to promote wound healing mainly from bums and to prevent a substantial number of illnesses. This study aimed to evaluate the antibacterial activity and toxicity of cosmetic cream containing Musa brachycarpa (MB) peels extracts. The antibacterial activity of both...

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Main Author: Maarof, Mas Haidatul Aqma
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2016
Subjects:
R Medicine
RA440-440.87 Study and teaching. Research
Online Access:http://eprints.usm.my/60717/1/MAS%20HAIDATUL%20AQMA%20BINTI%20MAAROF-E.pdf
http://eprints.usm.my/60717/
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spelling my.usm.eprints.60717 http://eprints.usm.my/60717/ Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation Maarof, Mas Haidatul Aqma R Medicine RA440-440.87 Study and teaching. Research Banana peels extracts have been used to promote wound healing mainly from bums and to prevent a substantial number of illnesses. This study aimed to evaluate the antibacterial activity and toxicity of cosmetic cream containing Musa brachycarpa (MB) peels extracts. The antibacterial activity of both aqueous and methanol extracts of Musa brachycarpa peels against Staphylococcus aureus (ATCC25923) and Staphylococcus epidermidis (A TCC 14990) was carried out using the disk diffusion and microbroth dilution methods. The aqueous extract of MB peel did not show inhibitory effect against the tested isolates. However methanol extract showed highest inhibitory effect against S. epidermidis with 14 mm inhibition zone diameter, followed by S. aureus with diameter of 12mm at 40mg/disc. Methanol extract of banana peels displayed a better antibacterial activity against both S. aureus and S. epidermidis compared to aqueous extract. Cream formulation shows inhibition in microbial growth with 10 mm inhibition against S.aureus and 12 mm against S. epidermidis. The MIC values of methanol extract against S. aureus and S. epidermidis were 80mg/ml. While the MIC value for aqueous extract was undetermined. The MBC values of methanol extract against S. aureus and S. epidermidis were 160 mg/ml each. Cytotoxic assay of the extracts was conducted using "Brine Shrimp Lethality" test (BLST). Methanol and aqueous extracts did not induce significant cytotoxic activity with LC50 value of 314.06 µg/mL and 604.98 µg/mL respectively after 24 hours. Heavy metal tests using atomic absorption spectroscopy (AAS) were performed to detect the presence of arsenic (As), lead (Pb), cadmium (Cd), chromium (Cr) and zinc (Zn) in the extracts and cosmetic cream. The level of heavy metals levels was within the acceptable range and safe to be used. This preliminary study has demostrated the antimicrobial potential and the safety of MB methanol extract to be applied for cosmetic use. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2016-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60717/1/MAS%20HAIDATUL%20AQMA%20BINTI%20MAAROF-E.pdf Maarof, Mas Haidatul Aqma (2016) Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation. Project Report. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
RA440-440.87 Study and teaching. Research
spellingShingle R Medicine
RA440-440.87 Study and teaching. Research
Maarof, Mas Haidatul Aqma
Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
description Banana peels extracts have been used to promote wound healing mainly from bums and to prevent a substantial number of illnesses. This study aimed to evaluate the antibacterial activity and toxicity of cosmetic cream containing Musa brachycarpa (MB) peels extracts. The antibacterial activity of both aqueous and methanol extracts of Musa brachycarpa peels against Staphylococcus aureus (ATCC25923) and Staphylococcus epidermidis (A TCC 14990) was carried out using the disk diffusion and microbroth dilution methods. The aqueous extract of MB peel did not show inhibitory effect against the tested isolates. However methanol extract showed highest inhibitory effect against S. epidermidis with 14 mm inhibition zone diameter, followed by S. aureus with diameter of 12mm at 40mg/disc. Methanol extract of banana peels displayed a better antibacterial activity against both S. aureus and S. epidermidis compared to aqueous extract. Cream formulation shows inhibition in microbial growth with 10 mm inhibition against S.aureus and 12 mm against S. epidermidis. The MIC values of methanol extract against S. aureus and S. epidermidis were 80mg/ml. While the MIC value for aqueous extract was undetermined. The MBC values of methanol extract against S. aureus and S. epidermidis were 160 mg/ml each. Cytotoxic assay of the extracts was conducted using "Brine Shrimp Lethality" test (BLST). Methanol and aqueous extracts did not induce significant cytotoxic activity with LC50 value of 314.06 µg/mL and 604.98 µg/mL respectively after 24 hours. Heavy metal tests using atomic absorption spectroscopy (AAS) were performed to detect the presence of arsenic (As), lead (Pb), cadmium (Cd), chromium (Cr) and zinc (Zn) in the extracts and cosmetic cream. The level of heavy metals levels was within the acceptable range and safe to be used. This preliminary study has demostrated the antimicrobial potential and the safety of MB methanol extract to be applied for cosmetic use.
format Monograph
author Maarof, Mas Haidatul Aqma
author_facet Maarof, Mas Haidatul Aqma
author_sort Maarof, Mas Haidatul Aqma
title Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
title_short Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
title_full Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
title_fullStr Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
title_full_unstemmed Antimicrobial activity and toxicity of Musa brachycarpa peel extracts for evaluation of cosmetic formulation
title_sort antimicrobial activity and toxicity of musa brachycarpa peel extracts for evaluation of cosmetic formulation
publisher Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate 2016
url http://eprints.usm.my/60717/1/MAS%20HAIDATUL%20AQMA%20BINTI%20MAAROF-E.pdf
http://eprints.usm.my/60717/
_version_ 1806443593615802368
score 13.211869

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