Staff View: P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract

P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract

The search for novel anticancer agents has moved from cytotoxic agents to biologically targeted agents (which are primarily antiproliferative) to avoid the traditional toxic effects of anticancer therapy and to develop more selective and effective agents. Inhibition of cancer cells growth is a ha...

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Main Author:	Radzuan, Syazatul Aniza
Format:	Monograph
Language:	English
Published:	Universiti Sains Malaysia 2015
Subjects:	R Medicine (General) R735-854 Medical education. Medical schools. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://eprints.usm.my/60651/1/SYAZATUL%20ANIZA%20BINTI%20RADZUAN%20-%20e.pdf http://eprints.usm.my/60651/
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id	my.usm.eprints.60651
record_format	eprints
spelling	my.usm.eprints.60651 http://eprints.usm.my/60651/ P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract Radzuan, Syazatul Aniza R Medicine (General) R735-854 Medical education. Medical schools. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer) The search for novel anticancer agents has moved from cytotoxic agents to biologically targeted agents (which are primarily antiproliferative) to avoid the traditional toxic effects of anticancer therapy and to develop more selective and effective agents. Inhibition of cancer cells growth is a hallmark for declining the cell proliferation status in the treatment of cancer. In this regard, herbals are more preferable for alternative agent to treat cancer. A plant gall, Quercus infectoria (QI) (manjakani) extract had been reported to have antiproliferative activity towards cervical cancer cell, Hela. However, its antiproliferative mechanism is remaining undiscovered. Therefore, the current study has utilized aqueous extract of Q. infectoria to investigate partial of antiproliferative mechanism demonstrated by previous study. The expression status of tumor suppressor protein p53 and human papilloma virus protein E7 treated samples were analyzed in this study. Preliminary study revealed the antiproliferative activity of the extract as I7.92± 0.42 pg/ml. This concentration was used to determine the morphological changes and nuclear fragmentation as hallmark of apoptosis by Hoechst stain. The protein expression of p53 and suppression of HPV-E7 both evaluated by Western Blotting method with respective different incubation time 24, 48 and 72 hours. Treated cells demonstrated the morphological changes and fragmented nuclear. The p53 proteins were expressed and HPV E7 was slightly suppressed. These results suggested that, QI aqueous extracts acted to suppress the Hela cells proliferation by induction of apoptosis with expression of p53 and suppression of HPV E7 Universiti Sains Malaysia 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60651/1/SYAZATUL%20ANIZA%20BINTI%20RADZUAN%20-%20e.pdf Radzuan, Syazatul Aniza (2015) P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract. Project Report. Universiti Sains Malaysia. (Submitted)
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	R Medicine (General) R735-854 Medical education. Medical schools. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	R Medicine (General) R735-854 Medical education. Medical schools. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Radzuan, Syazatul Aniza P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
description	The search for novel anticancer agents has moved from cytotoxic agents to biologically targeted agents (which are primarily antiproliferative) to avoid the traditional toxic effects of anticancer therapy and to develop more selective and effective agents. Inhibition of cancer cells growth is a hallmark for declining the cell proliferation status in the treatment of cancer. In this regard, herbals are more preferable for alternative agent to treat cancer. A plant gall, Quercus infectoria (QI) (manjakani) extract had been reported to have antiproliferative activity towards cervical cancer cell, Hela. However, its antiproliferative mechanism is remaining undiscovered. Therefore, the current study has utilized aqueous extract of Q. infectoria to investigate partial of antiproliferative mechanism demonstrated by previous study. The expression status of tumor suppressor protein p53 and human papilloma virus protein E7 treated samples were analyzed in this study. Preliminary study revealed the antiproliferative activity of the extract as I7.92± 0.42 pg/ml. This concentration was used to determine the morphological changes and nuclear fragmentation as hallmark of apoptosis by Hoechst stain. The protein expression of p53 and suppression of HPV-E7 both evaluated by Western Blotting method with respective different incubation time 24, 48 and 72 hours. Treated cells demonstrated the morphological changes and fragmented nuclear. The p53 proteins were expressed and HPV E7 was slightly suppressed. These results suggested that, QI aqueous extracts acted to suppress the Hela cells proliferation by induction of apoptosis with expression of p53 and suppression of HPV E7
format	Monograph
author	Radzuan, Syazatul Aniza
author_facet	Radzuan, Syazatul Aniza
author_sort	Radzuan, Syazatul Aniza
title	P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
title_short	P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
title_full	P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
title_fullStr	P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
title_full_unstemmed	P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract
title_sort	p53 and e7 expression status in human cervical carcinoma cell (hela) treated with quercusinfectoria aqueous extract
publisher	Universiti Sains Malaysia
publishDate	2015
url	http://eprints.usm.my/60651/1/SYAZATUL%20ANIZA%20BINTI%20RADZUAN%20-%20e.pdf http://eprints.usm.my/60651/
_version_	1800715759201026048
score	13.211869

P53 and E7 expression status in human cervical carcinoma cell (HELA) treated with quercusinfectoria aqueous extract

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