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The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users

Background: This study aimed to determine the differences in the mRNA and protein expression of ER stress sensors (such as BiP, XBP1, ATF4 and CHOP) in the peripheral blood mononuclear cells (PBMCs) between non-drug using healthy controls and regular kratom users, evaluate how alterations of ER stre...

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Main Author: Fauzi, Nurul Atiqah Muhamad
Format: Thesis
Language:English
Published: 2023
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/60349/1/SARA%20ABHARI%20-%20TESIS%20cut.pdf
http://eprints.usm.my/60349/
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spelling my.usm.eprints.60349 http://eprints.usm.my/60349/ The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users Fauzi, Nurul Atiqah Muhamad R5-920 Medicine (General) Background: This study aimed to determine the differences in the mRNA and protein expression of ER stress sensors (such as BiP, XBP1, ATF4 and CHOP) in the peripheral blood mononuclear cells (PBMCs) between non-drug using healthy controls and regular kratom users, evaluate how alterations of ER stress sensors expression was associated with the kratom use patterns (such as first age of using kratom, kratom use duration, kratom use average daily frequency, kratom use average daily quantity, and time of last kratom use prior to blood collection) and assess the correlations between the mRNA and protein expression of ER stress sensors in the peripheral blood mononuclear cells (PBMCs) among regular kratom users and the severity of depressive symptoms on kratom abstinence and kratom dependence severity. Methods: Data on socio-demographic characteristics were collected from kratom users and healthy controls. While data on kratom use patterns, kratom dependence severity and depressive symptoms on kratom abstinence were collected from kratom users. The expression of mRNA and protein of selected ER stress sensors (such as BiP, XBP1, ATF4 and CHOP) in the PBMCs of kratom users and healthy controls were measured with qRT-PCR and western blot, respectively. Results: A total of 20 kratom users and 20 healthy controls completed the study. The levels of expression of mRNA and protein of BiP were significantly elevated in kratom users compared with healthy controls regardless of the kratom use patterns. 2023-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60349/1/SARA%20ABHARI%20-%20TESIS%20cut.pdf Fauzi, Nurul Atiqah Muhamad (2023) The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Fauzi, Nurul Atiqah Muhamad
The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
description Background: This study aimed to determine the differences in the mRNA and protein expression of ER stress sensors (such as BiP, XBP1, ATF4 and CHOP) in the peripheral blood mononuclear cells (PBMCs) between non-drug using healthy controls and regular kratom users, evaluate how alterations of ER stress sensors expression was associated with the kratom use patterns (such as first age of using kratom, kratom use duration, kratom use average daily frequency, kratom use average daily quantity, and time of last kratom use prior to blood collection) and assess the correlations between the mRNA and protein expression of ER stress sensors in the peripheral blood mononuclear cells (PBMCs) among regular kratom users and the severity of depressive symptoms on kratom abstinence and kratom dependence severity. Methods: Data on socio-demographic characteristics were collected from kratom users and healthy controls. While data on kratom use patterns, kratom dependence severity and depressive symptoms on kratom abstinence were collected from kratom users. The expression of mRNA and protein of selected ER stress sensors (such as BiP, XBP1, ATF4 and CHOP) in the PBMCs of kratom users and healthy controls were measured with qRT-PCR and western blot, respectively. Results: A total of 20 kratom users and 20 healthy controls completed the study. The levels of expression of mRNA and protein of BiP were significantly elevated in kratom users compared with healthy controls regardless of the kratom use patterns.
format Thesis
author Fauzi, Nurul Atiqah Muhamad
author_facet Fauzi, Nurul Atiqah Muhamad
author_sort Fauzi, Nurul Atiqah Muhamad
title The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
title_short The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
title_full The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
title_fullStr The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
title_full_unstemmed The Intrinsic Role Of Endoplasmic Reticulum Stress Sensors In The Mechanism Underlying The Depressive Symptoms And Kratom Dependence In Chronic Kratom Users
title_sort intrinsic role of endoplasmic reticulum stress sensors in the mechanism underlying the depressive symptoms and kratom dependence in chronic kratom users
publishDate 2023
url http://eprints.usm.my/60349/1/SARA%20ABHARI%20-%20TESIS%20cut.pdf
http://eprints.usm.my/60349/
_version_ 1797907855700918272
score 13.211869

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