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Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver

Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular...

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Main Author: Omar, Abdullahi Abdirisak
Format: Thesis
Language:English
Published: 2023
Subjects:
R Medicine
RA440-440.87 Study and teaching. Research
RC109-216 Infectious and parasitic diseases
Online Access:http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf
http://eprints.usm.my/60284/
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spelling my.usm.eprints.60284 http://eprints.usm.my/60284/ Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver Omar, Abdullahi Abdirisak R Medicine RA440-440.87 Study and teaching. Research RC109-216 Infectious and parasitic diseases Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular identification. Infected cattle livers were collected from several farms in Kelantan. Morphometric parameters for juvenile, medium, and adult flukes such as body width (BW), body length (BL), cone width (CW), and cone length (CL) were determined using a calibrated stereomicroscope. Fluke genomic DNA was extracted using a QIAGEN Mini kit, and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) was performed on the 463 bp DNA fragment in the region of internal transcribed spacer 1 (ITS1) using Tas1 restriction enzyme. The PCR-RFLP product was separated on agarose gel and photographed, the livers were grossly investigated for Fasciola infection and morphological changes. Tissue samples were collected from histopathological examinations using H & E stain and the pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The morphometric analysis of adult flukes revealed a BL of 4.260 ± 0.191cm, BW of 0.61 ± 0.180 cm, cephalic CW of 0.37 ± 0.057 cm, and CL of 0.28 ± 0.058 cm. The BL/BW and BW/CW ratios were 2.93-5.97 cm and 1.55-2.72 cm respectively. The PCR-RFLP produced 3 bands of 93 bp, 151 bp, and 220 bp, which indicated as F. gigantica. The parasite was found in the liver and gall bladder, and thickening of bile duct mucous was observed on macroscopic examination. Microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in the epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy, and hyperplasia of the bladder epithelium. The study found that the combination of both morphometric analysis and molecular methods can accurately confirm the presence of F. gigantica in the infected liver. Histopathological characterisation has demonstrated different morphological features of infected cattle liver. 2023-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf Omar, Abdullahi Abdirisak (2023) Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
RA440-440.87 Study and teaching. Research
RC109-216 Infectious and parasitic diseases
spellingShingle R Medicine
RA440-440.87 Study and teaching. Research
RC109-216 Infectious and parasitic diseases
Omar, Abdullahi Abdirisak
Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
description Fasciola species, parasitic trematodes of mammals and humans, pose a significant public health risk and carry substantial socio-economic consequences. There have been few studies in Kelantan to characterise species, and this study focuses on their morphohistological characteristics and molecular identification. Infected cattle livers were collected from several farms in Kelantan. Morphometric parameters for juvenile, medium, and adult flukes such as body width (BW), body length (BL), cone width (CW), and cone length (CL) were determined using a calibrated stereomicroscope. Fluke genomic DNA was extracted using a QIAGEN Mini kit, and polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) was performed on the 463 bp DNA fragment in the region of internal transcribed spacer 1 (ITS1) using Tas1 restriction enzyme. The PCR-RFLP product was separated on agarose gel and photographed, the livers were grossly investigated for Fasciola infection and morphological changes. Tissue samples were collected from histopathological examinations using H & E stain and the pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The morphometric analysis of adult flukes revealed a BL of 4.260 ± 0.191cm, BW of 0.61 ± 0.180 cm, cephalic CW of 0.37 ± 0.057 cm, and CL of 0.28 ± 0.058 cm. The BL/BW and BW/CW ratios were 2.93-5.97 cm and 1.55-2.72 cm respectively. The PCR-RFLP produced 3 bands of 93 bp, 151 bp, and 220 bp, which indicated as F. gigantica. The parasite was found in the liver and gall bladder, and thickening of bile duct mucous was observed on macroscopic examination. Microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in the epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy, and hyperplasia of the bladder epithelium. The study found that the combination of both morphometric analysis and molecular methods can accurately confirm the presence of F. gigantica in the infected liver. Histopathological characterisation has demonstrated different morphological features of infected cattle liver.
format Thesis
author Omar, Abdullahi Abdirisak
author_facet Omar, Abdullahi Abdirisak
author_sort Omar, Abdullahi Abdirisak
title Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_short Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_full Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_fullStr Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_full_unstemmed Morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
title_sort morpho-histopathological characteristics and molecular identification of fasciola species from infected cattle liver
publishDate 2023
url http://eprints.usm.my/60284/1/Abdullahi%20Abdirisak%20Omar-E.pdf
http://eprints.usm.my/60284/
_version_ 1797907847942504448
score 13.211869

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