Association between peripheral white blood cell count and autologous skin flap healing rate in rabbits
Successful skin flap survival depends on the rate of healing process, which can be related to the roles of white blood cells (WBC). Therefore, the aim of this study was to investigate the association between WBC count and autologous skin flap healing rate in rabbits. A total of four New Zealand W...
Saved in:
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2015
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/60205/1/NORAZLINA%20BINTI%20HUSNI%20-%20e.pdf http://eprints.usm.my/60205/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Successful skin flap survival depends on the rate of healing process, which can be
related to the roles of white blood cells (WBC). Therefore, the aim of this study was to
investigate the association between WBC count and autologous skin flap healing rate in
rabbits. A total of four New Zealand White rabbits, aged between 9 to 15 months, were
used in this study. All rabbits underwent autologous skin flap surgery at the ventral
abdomen. A total of 3 ml of peripheral blood were collected from the central ear artery on day 0 (before surgery), day 1, 3, 5, 7 and 10 post-operation. The WBC count and manual WBC differential count were measured using Mythic 18 Vet Analyzer and
Wright’s stain, respectively. The skin flap healing rates were determined by mean
percentage of necrosis area at day 1, 3, 5, 7 and 10 post-operation. The results showed
that the post-operative mean percentage of necrosis area was not significantly associated
with the mean of WBC count. However, there was a decrease in the percentage of
necrosis area and the WBC count showed normalization pattern on post-operative day
10. These changes could suggest successful wound healing process. This study showed
that the pattern of WBC count changes could be used to evaluate the progress of wound healing in autologous skin flap surgery. |
|---|