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Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9

Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of re...

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Main Author: Manimaran, Janany
Format: Thesis
Language:English
Published: 2023
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf
http://eprints.usm.my/60182/
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spelling my.usm.eprints.60182 http://eprints.usm.my/60182/ Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9 Manimaran, Janany R5-920 Medicine (General) Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of research, the knowledge of andrographolide's anti-cancer effects on prostate cancer cells is relatively poor. In the current study, andrographolide was assessed on PC-3 cells, an aggressive androgen-independent prostate cancer cell line. Cytotoxicity analysis is vital in drug discovery research for assessing the biocompatibility of the drug being used on cancer cells. This work used the WST-1 assay to determine the cell survival of PC-3 cancer cells and Hs27 normal cells exposed to varying doses of andrographolide (0-200 μM). The results indicate that andrographolide dose-dependently suppresses the viability of PC-3 cells but not Hs27 cells. The NCI considers the LC50 value of 26.42 μM (after 48 hours of incubation) is acceptable. For the first time in this study, three different concentrations of andrographolide were used: control, half LC50, and LC50 (0, 13.21, and 26.42 μM) in all subsequent analyses. Metastasis is essential for the disease to progress; hence, the scratch assay and the transwell invasion assay were used to test andrographolide on PC-3 cells. 2023-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf Manimaran, Janany (2023) Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9. Masters thesis, Perpustakaan Hamzah Sendut.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Manimaran, Janany
Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
description Andrographolide is a labdane diterpenoid isolated from the plant Andrographis paniculata. This substance has numerous medicinal uses, notably anticancer effects. A previous study has revealed that andrographolide inhibits the growth of lung, brain, colon, and breast cancer cells. Due to a lack of research, the knowledge of andrographolide's anti-cancer effects on prostate cancer cells is relatively poor. In the current study, andrographolide was assessed on PC-3 cells, an aggressive androgen-independent prostate cancer cell line. Cytotoxicity analysis is vital in drug discovery research for assessing the biocompatibility of the drug being used on cancer cells. This work used the WST-1 assay to determine the cell survival of PC-3 cancer cells and Hs27 normal cells exposed to varying doses of andrographolide (0-200 μM). The results indicate that andrographolide dose-dependently suppresses the viability of PC-3 cells but not Hs27 cells. The NCI considers the LC50 value of 26.42 μM (after 48 hours of incubation) is acceptable. For the first time in this study, three different concentrations of andrographolide were used: control, half LC50, and LC50 (0, 13.21, and 26.42 μM) in all subsequent analyses. Metastasis is essential for the disease to progress; hence, the scratch assay and the transwell invasion assay were used to test andrographolide on PC-3 cells.
format Thesis
author Manimaran, Janany
author_facet Manimaran, Janany
author_sort Manimaran, Janany
title Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_short Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_full Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_fullStr Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_full_unstemmed Inhibitory Effects Of Andrographolide In Pc-3 Cell Line And The Induction Of Apoptosis Via The Involvement Of Caspases 3, 8 And 9
title_sort inhibitory effects of andrographolide in pc-3 cell line and the induction of apoptosis via the involvement of caspases 3, 8 and 9
publishDate 2023
url http://eprints.usm.my/60182/1/24%20Pages%20from%20JANANY%20AP%20MANIMARAN.pdf
http://eprints.usm.my/60182/
_version_ 1794552254835982336
score 13.211869

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