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Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant

Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the wa...

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Main Author: Rahman, Nur Hasyimah Abdul
Format: Thesis
Language:English
Published: 2022
Subjects:
QH1 Natural history (General - Including nature conservation, geographical distribution)
Online Access:http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf
http://eprints.usm.my/59874/
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spelling my.usm.eprints.59874 http://eprints.usm.my/59874/ Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant Rahman, Nur Hasyimah Abdul QH1 Natural history (General - Including nature conservation, geographical distribution) Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the waste materials and have a great chance to be a novel bioflocculant. Therefore, in this research, the methods for cultivation of Aureispira sp. CCB-QB1 inside a bioreactor was studied. Then, the biosorption activity of the dead cells toward heavy metals (Fe3+ and Cu2+) was tested. The QB1 dead cells were prepared by treating the living cells using 0.5 % of formaldehyde. The dead cells were discovered to show robust flocculating activity toward kaolin (initial concentration 0.2 %) in the presence of 7 mM CaCl2. For cultivation inside flasks, 5 ml of the dead cell suspension showed overall of 96 % of flocculating activity after shaken using both 150 and 200 rpm agitation speeds. Besides, both tryptone and peptone were found to highly supported the growth of QB1 cells. The presence of peptone in the cell culture also helps in the aggregation of the QB1 cells. For cultivation inside a bioreactor, QB1 culture conditions were optimized using Response Surface Methodology (RSM). The optimized conditions were 3.39 % of sea salt concentration, 0.9 % of peptone concentration, and 29 h of incubation period. Additionally 2022-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf Rahman, Nur Hasyimah Abdul (2022) Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Rahman, Nur Hasyimah Abdul
Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
description Bioflocculants are important to remove pollutants, especially organic particles and heavy metals from wastewater. Previous study showed that Aureispira sp. CCB-QB1 demonstrated cell aggregation when calcium ions are available. This suggested that these bacterial cells are capable to adsorb the waste materials and have a great chance to be a novel bioflocculant. Therefore, in this research, the methods for cultivation of Aureispira sp. CCB-QB1 inside a bioreactor was studied. Then, the biosorption activity of the dead cells toward heavy metals (Fe3+ and Cu2+) was tested. The QB1 dead cells were prepared by treating the living cells using 0.5 % of formaldehyde. The dead cells were discovered to show robust flocculating activity toward kaolin (initial concentration 0.2 %) in the presence of 7 mM CaCl2. For cultivation inside flasks, 5 ml of the dead cell suspension showed overall of 96 % of flocculating activity after shaken using both 150 and 200 rpm agitation speeds. Besides, both tryptone and peptone were found to highly supported the growth of QB1 cells. The presence of peptone in the cell culture also helps in the aggregation of the QB1 cells. For cultivation inside a bioreactor, QB1 culture conditions were optimized using Response Surface Methodology (RSM). The optimized conditions were 3.39 % of sea salt concentration, 0.9 % of peptone concentration, and 29 h of incubation period. Additionally
format Thesis
author Rahman, Nur Hasyimah Abdul
author_facet Rahman, Nur Hasyimah Abdul
author_sort Rahman, Nur Hasyimah Abdul
title Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_short Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_full Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_fullStr Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_full_unstemmed Biomass Production Of Aureispira Sp. Ccb-qb1 And Its Application As A Novel Bioflocculant
title_sort biomass production of aureispira sp. ccb-qb1 and its application as a novel bioflocculant
publishDate 2022
url http://eprints.usm.my/59874/1/NUR%20HASYIMAH%20BINTI%20ABDUL%20RAHMAN%20-%20TESIS24.pdf
http://eprints.usm.my/59874/
_version_ 1792151211754913792
score 13.211869

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