9th International conference on biosience, biochemistry & bioinformatics 2018
Transient receptor potential cation channel subfamily M member 4 (TRPM4) is overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL) associated with poor survival. In this study, its functions in the disease and the potency of its inhibitor 9-phenanthrol were in...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Perubatan
2019
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| Subjects: | |
| Online Access: | http://eprints.usm.my/59762/1/DR%20WONG%20KAH%20KENG.pdf http://eprints.usm.my/59762/ |
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| Summary: | Transient receptor potential cation channel subfamily M member 4 (TRPM4) is
overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL)
associated with poor survival. In this study, its functions in the disease and the potency of its
inhibitor 9-phenanthrol were investigated. The biological functions associated with TR.PM4 mRNA
expression were examined through Gene Set Enrichment Analysis (GSEA) in ABC-DLBCL cases
(n=15). The cytotoxicity of 9-phenanthrol in three ABC-DLBCL cell lines (SUDHL2, OCI-LY3,
OCI-LYIO) was tested at six different concentrations (0.0InM, 0.1 nM, InM, lOnM, 25nM, 50nM).
GSEA results showed that cell cycle gene sets conferred the highest number of gene sets
representing 42% (n=21/50) of the top 50 most significantly enriched gene sets ranked according to
false discovery rate (FDR; all 50 gene sets had FDRO.OI), followed by DNA replication (n=8/50;
16%) and RNA processing (n=8/50; 16%), suggesting the roles of TRPM4 in cell cycle progression
and cellular division of ABC-DLBCL. In terms of the cytotoxicity effects of 9-phenanthrol, the
resulting GI50 for all ABC-DLBCL cell lines ranged from 19nM-41,88nM. In conclusion, TRPM4 is
potentially involved in the cell cycle progression and cellular division of ABC-DLBCL cells, and
the TRPM4 inhibitor 9-phenanthrol was cytotoxic against ABC-DLBCL cells. |
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