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Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma

The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolutio...

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Main Author: Sauji, Nadiratul Asyikin
Format: Thesis
Language:English
Published: 2021
Subjects:
QD71-142 Analytical chemistry
Online Access:http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf
http://eprints.usm.my/58282/
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spelling my.usm.eprints.58282 http://eprints.usm.my/58282/ Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma Sauji, Nadiratul Asyikin QD71-142 Analytical chemistry The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolution liquid chromatography (RRLC) was used to analyse cyanidin-3-O-glucoside chloride, one of the anthocyanins found in roselle. Spectrophotometric analysis and optimization of chromatographic conditions were performed to develop a suitable and precise RRLC method for the determination of the selected anthocyanin. Both liquid-liquid extraction (LLE) and solid-phase extraction (SPE) method were performed to evaluate the best extraction method of anthocyanin in the rat plasma sample. The optimised chromatographic method demonstrated that the mobile phase composition of 0.1% trifluoroacetic acid aqueous solution and acetonitrile in the ratio of 81:19, respectively with a flow rate of 0.5 mL/min, at 30°C column temperature and detection wavelength of 525 nm were suitable for RRLC analysis of cyanidin-3-O-glucoside chloride. SPE was chosen as the final extraction method in the study as it produced better peaks in the chromatogram compared to LLE. In conclusion, the developed RRLC method in this study can be used to determine anthocyanins in the future pharmacokinetic study of roselle. 2021-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf Sauji, Nadiratul Asyikin (2021) Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QD71-142 Analytical chemistry
spellingShingle QD71-142 Analytical chemistry
Sauji, Nadiratul Asyikin
Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
description The growing interest of anthocyanins in roselle has brought upon the importance of the compound’s analysis to investigate its pharmacological properties. Sensitive and specific analytical methods are required to accurately analyse the anthocyanin present in the sample. In this study, rapid resolution liquid chromatography (RRLC) was used to analyse cyanidin-3-O-glucoside chloride, one of the anthocyanins found in roselle. Spectrophotometric analysis and optimization of chromatographic conditions were performed to develop a suitable and precise RRLC method for the determination of the selected anthocyanin. Both liquid-liquid extraction (LLE) and solid-phase extraction (SPE) method were performed to evaluate the best extraction method of anthocyanin in the rat plasma sample. The optimised chromatographic method demonstrated that the mobile phase composition of 0.1% trifluoroacetic acid aqueous solution and acetonitrile in the ratio of 81:19, respectively with a flow rate of 0.5 mL/min, at 30°C column temperature and detection wavelength of 525 nm were suitable for RRLC analysis of cyanidin-3-O-glucoside chloride. SPE was chosen as the final extraction method in the study as it produced better peaks in the chromatogram compared to LLE. In conclusion, the developed RRLC method in this study can be used to determine anthocyanins in the future pharmacokinetic study of roselle.
format Thesis
author Sauji, Nadiratul Asyikin
author_facet Sauji, Nadiratul Asyikin
author_sort Sauji, Nadiratul Asyikin
title Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_short Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_full Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_fullStr Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_full_unstemmed Development and optimization of rapid resolution liquid chromatography (RRLC) method following solid-phase extraction for determination of anthocyanin in rat plasma
title_sort development and optimization of rapid resolution liquid chromatography (rrlc) method following solid-phase extraction for determination of anthocyanin in rat plasma
publishDate 2021
url http://eprints.usm.my/58282/1/Nadiratul-24%20pages.pdf
http://eprints.usm.my/58282/
_version_ 1768007872294158336
score 13.211869

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