Characterization of periodontal fibroblast spheroids in vitro, interaction with 3d membranes and potential for tissue regeneration
Autologous transplantation of periodontal fibroblasts may be a promising technique to induce tissue regeneration In the treatment of periodontal disease. Spheroid culture is a form of three dimensional cell culture that promotes cellmatrix interaction and cellular differentiation without recourse...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2012
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| Subjects: | |
| Online Access: | http://eprints.usm.my/57307/1/DR%20ZURAIRAD%20BERAHIM.pdf http://eprints.usm.my/57307/ |
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| Summary: | Autologous transplantation of periodontal fibroblasts may be a promising
technique to induce tissue regeneration In the treatment of periodontal disease. Spheroid
culture is a form of three dimensional cell culture that promotes cellmatrix interaction and
cellular differentiation without recourse to exogenous growth factors. The aim of this study
was to develop 3D spheroidal cultures of periodontal fibroblasts In vitro and characterize
them with respect to their potential use in periodontal tissue repair and rege09!"8tion. In
this study commercial normal periodontal fibroblasts were grown in spheroidal form In vitro
using the liquid overlay technique. The fibroblast spheroids were characterized by
histology, DNA quantification, scanning electron microscopy and immunohistochemistry.
Over 14 days, periodontal fibroblasts formed viable spheroids which Incorporated an
extracellular matrix rich in collagen type I and periostln. Production of cementum
attachment protein which is a marker of dental hard tissue formation, was not detected.
This study shows that periodontal fibroblast spheroids may have the potential to be used
as an adjunct for periodontal regeneration. |
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