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Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium

The distinction between an endocervical adenocarcinoma (ECA) and an endometrial adenocarcinoma (EMA) can be problematic on small biopsies or when there is tumor in both endocervical and endometrial specimens or when the tumor has extended into the lower uterine segment. The judgment is difficult...

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Main Author: Farveen Marican, Abu Backer Maricar
Format: Thesis
Language:English
Published: 2010
Subjects:
R Medicine (General)
Online Access:http://eprints.usm.my/56135/1/DR%20FARVEEN%20MARICAN%20ABU%20BACKER%20MARICAR%20-%20e.pdf
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spelling my.usm.eprints.56135 http://eprints.usm.my/56135/ Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium Farveen Marican, Abu Backer Maricar R Medicine (General) The distinction between an endocervical adenocarcinoma (ECA) and an endometrial adenocarcinoma (EMA) can be problematic on small biopsies or when there is tumor in both endocervical and endometrial specimens or when the tumor has extended into the lower uterine segment. The judgment is difficult to be based on histomorphology alone because these tumors can have similar histologic appearance. We investigated the value of pl6INK4a, p21WAFl and p27kipl immunohistochemistry for distinguishing ECA and an EMA. We immunostained tissue sections of archival samples from 2005 to 2008 from HUSM and HSB. The immunohistochemical staining scores were correlated with their clinicopathologic parameters. There were 40 ECA and 92 EMA cases examined. We observed significant higher expressions of pl6INK4a and p27kipl ([p <0.001] (80% versus 25%) and [p=0.001] (43% versus 15%)) in ECA than in EMA. ECA could be differentiated from EMA based on the combination expressions of pl6INK4a and p 27kipl. p21WAFl expression did not differentiate these two carcinomas (70% versus 78%, p=0.312). There was significant association seen between negative pl6INK4a expression and low histologic grade in EMA (p=0.014). In ECA, p21WAFl expression shows significant association with corpus infiltration (p-0.043) while negative p27kipl expression with lymph node invasion (p=0.030). Multivariate analysis however shows no association between lymph node invasion and p27kipl expression adjusted by race, histologic grade, vascular invasion, p21WAF1 expression and extension into the uterine corpus. In conclusions, combination of p!6INK4a and p27kipl expression is helpful in differentiating ECA from EMA. In small biopsy, the expression of p21WAFl may help in assessing the presence of corpus infiltration. P27kipl expression is helpful in predicting presence of lymph node invasion. 2010 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/56135/1/DR%20FARVEEN%20MARICAN%20ABU%20BACKER%20MARICAR%20-%20e.pdf Farveen Marican, Abu Backer Maricar (2010) Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Farveen Marican, Abu Backer Maricar
Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
description The distinction between an endocervical adenocarcinoma (ECA) and an endometrial adenocarcinoma (EMA) can be problematic on small biopsies or when there is tumor in both endocervical and endometrial specimens or when the tumor has extended into the lower uterine segment. The judgment is difficult to be based on histomorphology alone because these tumors can have similar histologic appearance. We investigated the value of pl6INK4a, p21WAFl and p27kipl immunohistochemistry for distinguishing ECA and an EMA. We immunostained tissue sections of archival samples from 2005 to 2008 from HUSM and HSB. The immunohistochemical staining scores were correlated with their clinicopathologic parameters. There were 40 ECA and 92 EMA cases examined. We observed significant higher expressions of pl6INK4a and p27kipl ([p <0.001] (80% versus 25%) and [p=0.001] (43% versus 15%)) in ECA than in EMA. ECA could be differentiated from EMA based on the combination expressions of pl6INK4a and p 27kipl. p21WAFl expression did not differentiate these two carcinomas (70% versus 78%, p=0.312). There was significant association seen between negative pl6INK4a expression and low histologic grade in EMA (p=0.014). In ECA, p21WAFl expression shows significant association with corpus infiltration (p-0.043) while negative p27kipl expression with lymph node invasion (p=0.030). Multivariate analysis however shows no association between lymph node invasion and p27kipl expression adjusted by race, histologic grade, vascular invasion, p21WAF1 expression and extension into the uterine corpus. In conclusions, combination of p!6INK4a and p27kipl expression is helpful in differentiating ECA from EMA. In small biopsy, the expression of p21WAFl may help in assessing the presence of corpus infiltration. P27kipl expression is helpful in predicting presence of lymph node invasion.
format Thesis
author Farveen Marican, Abu Backer Maricar
author_facet Farveen Marican, Abu Backer Maricar
author_sort Farveen Marican, Abu Backer Maricar
title Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
title_short Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
title_full Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
title_fullStr Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
title_full_unstemmed Expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
title_sort expressions of pl6ink4a, p27kipl and p21 waf1 in differentiating primary adenocarcinoma of endocervix from endometrium
publishDate 2010
url http://eprints.usm.my/56135/1/DR%20FARVEEN%20MARICAN%20ABU%20BACKER%20MARICAR%20-%20e.pdf
http://eprints.usm.my/56135/
_version_ 1753789243753234432
score 13.211869

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