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Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems

Biotransformation is the process through which the functional groups of organic compounds are modified by living cells to a chemically different product. The procedure investigates the unique qualities of biological catalysts, such as stereospecificity and area specificity, as well as their capacity...

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Main Author: Zulkifli, Umy Aliya
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2022
Subjects:
T Technology
TP155-156 Chemical engineering
Online Access:http://eprints.usm.my/55650/1/Biotransformation%20Of%20Valencene%20Utilizing%20Yarrowia%20Lipolytica%20In%20Buffer%20Systems.pdf
http://eprints.usm.my/55650/
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spelling my.usm.eprints.55650 http://eprints.usm.my/55650/ Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems Zulkifli, Umy Aliya T Technology TP155-156 Chemical engineering Biotransformation is the process through which the functional groups of organic compounds are modified by living cells to a chemically different product. The procedure investigates the unique qualities of biological catalysts, such as stereospecificity and area specificity, as well as their capacity to endure reactions at no severe temperatures or pH values. In the biotransformation of (+)-valencene to produce (+)-nootkatone, Y. lipolytica is used as a biocatalyst for the oxidation of barely soluble hydrophobic steroids. The parameters such as in disodium phosphate and monosodium phosphate buffer system are manipulated to determine the optimum condition for (+)-nootkatone synthesis. GC-MS analysis was used to detect the results or the peak area of (+)- nootkatone and (+)- valencene. The growth curve of Y. lipolytica was determined, and the best growth phase for biotransformation was in the exponential phase at 22 h. The maximum Wdcw concentration is 5.70 g/L was observed at 55 h in the stationary phase. Biotransformation of (+)-valencene utilizing Y. lipolytica in buffer systems is done successfully. The maximum (+)- nootkatone area was observed at pH 6 by adding 250 μL (+)- valencene and cultivating at 30 ℃, 150 rpm. This study concludes that the buffer system's pH significantly impacts the utilization of yeast, Y. lipolytica, during biotransformation. The optimal pH for the fastest reaction rate and maximum production for the created cultures were discovered. The (+)- nootkatone production has been studied at pH 6, and it has the most significant peak compared to the others. Universiti Sains Malaysia 2022-07-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/55650/1/Biotransformation%20Of%20Valencene%20Utilizing%20Yarrowia%20Lipolytica%20In%20Buffer%20Systems.pdf Zulkifli, Umy Aliya (2022) Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic T Technology
TP155-156 Chemical engineering
spellingShingle T Technology
TP155-156 Chemical engineering
Zulkifli, Umy Aliya
Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
description Biotransformation is the process through which the functional groups of organic compounds are modified by living cells to a chemically different product. The procedure investigates the unique qualities of biological catalysts, such as stereospecificity and area specificity, as well as their capacity to endure reactions at no severe temperatures or pH values. In the biotransformation of (+)-valencene to produce (+)-nootkatone, Y. lipolytica is used as a biocatalyst for the oxidation of barely soluble hydrophobic steroids. The parameters such as in disodium phosphate and monosodium phosphate buffer system are manipulated to determine the optimum condition for (+)-nootkatone synthesis. GC-MS analysis was used to detect the results or the peak area of (+)- nootkatone and (+)- valencene. The growth curve of Y. lipolytica was determined, and the best growth phase for biotransformation was in the exponential phase at 22 h. The maximum Wdcw concentration is 5.70 g/L was observed at 55 h in the stationary phase. Biotransformation of (+)-valencene utilizing Y. lipolytica in buffer systems is done successfully. The maximum (+)- nootkatone area was observed at pH 6 by adding 250 μL (+)- valencene and cultivating at 30 ℃, 150 rpm. This study concludes that the buffer system's pH significantly impacts the utilization of yeast, Y. lipolytica, during biotransformation. The optimal pH for the fastest reaction rate and maximum production for the created cultures were discovered. The (+)- nootkatone production has been studied at pH 6, and it has the most significant peak compared to the others.
format Monograph
author Zulkifli, Umy Aliya
author_facet Zulkifli, Umy Aliya
author_sort Zulkifli, Umy Aliya
title Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
title_short Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
title_full Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
title_fullStr Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
title_full_unstemmed Biotransformation Of Valencene Utilizing Yarrowia Lipolytica In Buffer Systems
title_sort biotransformation of valencene utilizing yarrowia lipolytica in buffer systems
publisher Universiti Sains Malaysia
publishDate 2022
url http://eprints.usm.my/55650/1/Biotransformation%20Of%20Valencene%20Utilizing%20Yarrowia%20Lipolytica%20In%20Buffer%20Systems.pdf
http://eprints.usm.my/55650/
_version_ 1751537255771537408
score 13.211869

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