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Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle

The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhyd...

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Main Author: Arumugam, Deyvanai
Format: Thesis
Language:English
Published: 2021
Subjects:
QH1-278.5 Natural history (General)
Online Access:http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf
http://eprints.usm.my/55086/
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spelling my.usm.eprints.55086 http://eprints.usm.my/55086/ Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle Arumugam, Deyvanai QH1-278.5 Natural history (General) The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhydroxyalkanoate. Based on the criteria of fast growing, plasmid stability and ability to grow on minimal medium, strain JM109(DE3) was found to be the most suitable host for PHA production from xylose. The two plasmids were found to be stable after 10 serial overnight subcultures even without antibiotics selection. The strain was able to accumulate PHA up 62% CDW after 120 hours of incubation in mineral medium containing 1% xylose. Increasing xylose concentration up to 5% did not cause any further increase in PHA content, nor toxicity. Even at 1% xylose or glucose, the data suggests that the mineral medium was already limiting. When grown in 1% glucose, JM109(DE3) yield only 12% CDW PHA. Increasing glucose concentration to 5% leads to lower PHA content of only 6% CDW, implying glucose inhibition. In an experiment using a range of xylose:glucose ratio of 1:0.1 to 1:1, the PHA content fell from 58% to 32% CDW. In order to overcome glucose toxicity, six XylE mutants were tested for PHA production. 2021-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf Arumugam, Deyvanai (2021) Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle. Masters thesis, Perpustakaan Hamzah Sendut.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Arumugam, Deyvanai
Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
description The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhydroxyalkanoate. Based on the criteria of fast growing, plasmid stability and ability to grow on minimal medium, strain JM109(DE3) was found to be the most suitable host for PHA production from xylose. The two plasmids were found to be stable after 10 serial overnight subcultures even without antibiotics selection. The strain was able to accumulate PHA up 62% CDW after 120 hours of incubation in mineral medium containing 1% xylose. Increasing xylose concentration up to 5% did not cause any further increase in PHA content, nor toxicity. Even at 1% xylose or glucose, the data suggests that the mineral medium was already limiting. When grown in 1% glucose, JM109(DE3) yield only 12% CDW PHA. Increasing glucose concentration to 5% leads to lower PHA content of only 6% CDW, implying glucose inhibition. In an experiment using a range of xylose:glucose ratio of 1:0.1 to 1:1, the PHA content fell from 58% to 32% CDW. In order to overcome glucose toxicity, six XylE mutants were tested for PHA production.
format Thesis
author Arumugam, Deyvanai
author_facet Arumugam, Deyvanai
author_sort Arumugam, Deyvanai
title Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_short Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_full Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_fullStr Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_full_unstemmed Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_sort production of polyhydroxyalkanoates from xylose by escherichia coli carrying wild type and mutant xyle
publishDate 2021
url http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf
http://eprints.usm.my/55086/
_version_ 1746211084866420736
score 13.211869

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