Cover Image

Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves

Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, cur...

Full description

Saved in:
Bibliographic Details
Main Author: Tan, Li Vern
Format: Thesis
Language:English
Published: 2021
Subjects:
QH1 Natural history (General - Including nature conservation, geographical distribution)
Online Access:http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf
http://eprints.usm.my/55059/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.55059
record_format eprints
spelling my.usm.eprints.55059 http://eprints.usm.my/55059/ Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves Tan, Li Vern QH1 Natural history (General - Including nature conservation, geographical distribution) Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, current conventional cultivation methods applied for the propagation of plant stocks are restricted due to the difficulty in obtaining a large number of well-rooted, disease-free plantlets. Plant tissue culture particularly micropropagation has been successfully applied to propagate plant stocks for many commercial crops. The current study aims to establish an efficient protocol for the micropropagation of Ficus carica cv. Texas Everbearing (TE) and Lisa and to evaluate the anti-hyperglycemic potential of fig leaves. From the results obtained, explant surface sterilization was successfully performed with a maximum survival rate of 50%. The optimal shoot induction condition for the cultivar TE was nodal segments cultured horizontally in MS medium supplemented with 1.0 g/L AC, 10 g/L sucrose, 3.0 mg/L TDZ and 0.75 mg/L NAA, recording maximum shoot induction rate (100%), shoot number of 2.15 ± 0.10 shoots and shoot length of 1.05 ± 0.12 cm. For the cultivar Lisa, shoot induction rate of 100%, shoot number of 1.92 ± 0.14 shoots and shoot length of 2.11 ± 0.18 cm was obtained similarly by culturing nodal segments horizontally in MS medium supplemented with 1.0 g/L AC but with 20 g/L fructose, 2.0 mg/L ZEA and 1.0 mg/L NAA. Explants of both cultivars were then successfully rooted in WPM and acclimatized in a potting mix comprising of black soil, red soil and coco peat at a 3:1:1 ratio. In the evaluation of anti-hyperglycemic potential of fig leaves from F. carica cv. TE and Lisa on diabetic induced rats, leaf extracts were obtained via maceration in 50% ethanol for 72 hours. Leaf extract from fig cultivar TE and Lisa at 250 mg/kg showed a significant effect (p < 0.05) in the reduction fasting blood glucose level at day 14 (16.3 ± 2.1 and 17.6 ± 3.1 mmol/L respectively) and average water intake in week 2 (27.2 ± 2.4 and 27.5 ± 2.0 ml/100 g b.w. respectively) when compared to vehicle treated diabetic control, all reflecting a reduction in hyperglycemia. No significant difference was recorded on the body and organ weights when compared to diabetic control rats. This study has successfully documented an efficient micropropagation protocol for fig (Ficus carica) cultivars ‘Texas Everbearing’ and ‘Lisa’ for the production of plant stocks suitable for commercial field establishments and also highlights the potential of fig leaf extract from both fig cultivars in controlling diabetes. 2021-10 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf Tan, Li Vern (2021) Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves. PhD thesis, Universiti Sains Malaysia..
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Tan, Li Vern
Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
description Common figs (Ficus carica) are highly valued fruit crops owing to its elevated nutrient content and vast economic and medicinal importance. Although native to the Middle East and Mediterranean region, the adaptability of figs has attributed to its cultivation also in tropical countries. However, current conventional cultivation methods applied for the propagation of plant stocks are restricted due to the difficulty in obtaining a large number of well-rooted, disease-free plantlets. Plant tissue culture particularly micropropagation has been successfully applied to propagate plant stocks for many commercial crops. The current study aims to establish an efficient protocol for the micropropagation of Ficus carica cv. Texas Everbearing (TE) and Lisa and to evaluate the anti-hyperglycemic potential of fig leaves. From the results obtained, explant surface sterilization was successfully performed with a maximum survival rate of 50%. The optimal shoot induction condition for the cultivar TE was nodal segments cultured horizontally in MS medium supplemented with 1.0 g/L AC, 10 g/L sucrose, 3.0 mg/L TDZ and 0.75 mg/L NAA, recording maximum shoot induction rate (100%), shoot number of 2.15 ± 0.10 shoots and shoot length of 1.05 ± 0.12 cm. For the cultivar Lisa, shoot induction rate of 100%, shoot number of 1.92 ± 0.14 shoots and shoot length of 2.11 ± 0.18 cm was obtained similarly by culturing nodal segments horizontally in MS medium supplemented with 1.0 g/L AC but with 20 g/L fructose, 2.0 mg/L ZEA and 1.0 mg/L NAA. Explants of both cultivars were then successfully rooted in WPM and acclimatized in a potting mix comprising of black soil, red soil and coco peat at a 3:1:1 ratio. In the evaluation of anti-hyperglycemic potential of fig leaves from F. carica cv. TE and Lisa on diabetic induced rats, leaf extracts were obtained via maceration in 50% ethanol for 72 hours. Leaf extract from fig cultivar TE and Lisa at 250 mg/kg showed a significant effect (p < 0.05) in the reduction fasting blood glucose level at day 14 (16.3 ± 2.1 and 17.6 ± 3.1 mmol/L respectively) and average water intake in week 2 (27.2 ± 2.4 and 27.5 ± 2.0 ml/100 g b.w. respectively) when compared to vehicle treated diabetic control, all reflecting a reduction in hyperglycemia. No significant difference was recorded on the body and organ weights when compared to diabetic control rats. This study has successfully documented an efficient micropropagation protocol for fig (Ficus carica) cultivars ‘Texas Everbearing’ and ‘Lisa’ for the production of plant stocks suitable for commercial field establishments and also highlights the potential of fig leaf extract from both fig cultivars in controlling diabetes.
format Thesis
author Tan, Li Vern
author_facet Tan, Li Vern
author_sort Tan, Li Vern
title Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_short Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_full Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_fullStr Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_full_unstemmed Micropropagation Of Fig (Ficus Carica) Cultivars ‘texas Everbearing’ And ‘lisa’ & The Anti-hyperglycemic Potential Of Fig Leaves
title_sort micropropagation of fig (ficus carica) cultivars ‘texas everbearing’ and ‘lisa’ & the anti-hyperglycemic potential of fig leaves
publishDate 2021
url http://eprints.usm.my/55059/1/TAN%20LI%20VERN%20-%20TESIS24.pdf
http://eprints.usm.my/55059/
_version_ 1745563226692648960
score 13.211869

Similar Items