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Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification

Ester is an aromatic compound naturally found in plants and fruits, which is animportant additive for food, beverages, pharmaceutical and cosmetic industries, giving flavour and fragrance. The compound is either supplied by natural resources or chemical synthesis. Lipase as biocatalyst shows great...

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Main Author: Sim, Wan Ting
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2021
Subjects:
T Technology
TP Chemical Technology
Online Access:http://eprints.usm.my/54738/1/Synthesis%20Of%20Butyl%20Acetate%20By%20Lipase-Catalysed%20Esterification_Sim%20Wan%20Ting_K4_2021_ESAR.pdf
http://eprints.usm.my/54738/
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spelling my.usm.eprints.54738 http://eprints.usm.my/54738/ Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification Sim, Wan Ting T Technology TP Chemical Technology Ester is an aromatic compound naturally found in plants and fruits, which is animportant additive for food, beverages, pharmaceutical and cosmetic industries, giving flavour and fragrance. The compound is either supplied by natural resources or chemical synthesis. Lipase as biocatalyst shows great potential in aroma ester synthesis since lipase-catalysed reaction product is verified to be natural. In this study, butyl acetate was synthesised from acetic acid and n-butanol esterification catalysed by immobilized Candida rugosa lipase. Theprocess parameters which were optimized included reaction time, enzyme amount, temperature, and acetic acid/ethanol molar ratio were studied using one-factor-at-a-time (OFAT) method. Optimal molar conversion for butyl acetate of 81.33 % was obtained at 40 minutes incubation time with 50 U/ml of enzyme amount and substrate molar ratio of 1:1 (0.1 M) at 40 ℃ and 150 rpm. The kinetic parameters were also calculated using secondary data extracted from another researcher group. The data was fitted into Random Bi Bi, Ordered Bi Bi and Ping Pong Bi Bi mechanisms to compare the kinetic parameters obtained. The kinetic analysis of the esterification reactions was found to follow a Ping Pong Bi Bi mechanism with inhibition by one of the substrates, acetic acid. The fitted kinetic parameters obtained were Vmax = 249.05 mol/min.g, K m(A) = 46.25 mol/dm3 , K m(B) = 4.15 mol/dm3, KI = 15.09 mol/dm3. Universiti Sains Malaysia 2021-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/54738/1/Synthesis%20Of%20Butyl%20Acetate%20By%20Lipase-Catalysed%20Esterification_Sim%20Wan%20Ting_K4_2021_ESAR.pdf Sim, Wan Ting (2021) Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic T Technology
TP Chemical Technology
spellingShingle T Technology
TP Chemical Technology
Sim, Wan Ting
Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
description Ester is an aromatic compound naturally found in plants and fruits, which is animportant additive for food, beverages, pharmaceutical and cosmetic industries, giving flavour and fragrance. The compound is either supplied by natural resources or chemical synthesis. Lipase as biocatalyst shows great potential in aroma ester synthesis since lipase-catalysed reaction product is verified to be natural. In this study, butyl acetate was synthesised from acetic acid and n-butanol esterification catalysed by immobilized Candida rugosa lipase. Theprocess parameters which were optimized included reaction time, enzyme amount, temperature, and acetic acid/ethanol molar ratio were studied using one-factor-at-a-time (OFAT) method. Optimal molar conversion for butyl acetate of 81.33 % was obtained at 40 minutes incubation time with 50 U/ml of enzyme amount and substrate molar ratio of 1:1 (0.1 M) at 40 ℃ and 150 rpm. The kinetic parameters were also calculated using secondary data extracted from another researcher group. The data was fitted into Random Bi Bi, Ordered Bi Bi and Ping Pong Bi Bi mechanisms to compare the kinetic parameters obtained. The kinetic analysis of the esterification reactions was found to follow a Ping Pong Bi Bi mechanism with inhibition by one of the substrates, acetic acid. The fitted kinetic parameters obtained were Vmax = 249.05 mol/min.g, K m(A) = 46.25 mol/dm3 , K m(B) = 4.15 mol/dm3, KI = 15.09 mol/dm3.
format Monograph
author Sim, Wan Ting
author_facet Sim, Wan Ting
author_sort Sim, Wan Ting
title Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
title_short Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
title_full Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
title_fullStr Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
title_full_unstemmed Synthesis Of Butyl Acetate By Lipase-Catalysed Esterification
title_sort synthesis of butyl acetate by lipase-catalysed esterification
publisher Universiti Sains Malaysia
publishDate 2021
url http://eprints.usm.my/54738/1/Synthesis%20Of%20Butyl%20Acetate%20By%20Lipase-Catalysed%20Esterification_Sim%20Wan%20Ting_K4_2021_ESAR.pdf
http://eprints.usm.my/54738/
_version_ 1744354464698990592
score 13.251813

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